Literature DB >> 2186372

A 3.5 genome equivalent multi access YAC library: construction, characterisation, screening and storage.

R Anand1, J H Riley, R Butler, J C Smith, A F Markham.   

Abstract

The construction of a yeast artificial chromosome (YAC) primary gridded library of 35,000 clones from human lymphoblastoid (48,XXXX) cell line DNA is described. The average YAC size is approximately 350kb representing a greater than 3.5 times coverage of the genome. The library is stored at -70 degrees C as gridded clones on nylon filters impregnated with 20% glycerol and as glycerol suspensions of individual clones in microtitre plates providing a prolonged multi-user potential. To date we have used 14 single copy probes to screen this library by colony hybridisation as well as PCR and have isolated between 1 and 5 YAC clones for every probe.

Entities:  

Mesh：

Year:  1990        PMID： 2186372      PMCID： PMC330667          DOI： 10.1093/nar/18.8.1951

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  17 in total

1.  Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.

Authors:  D T Burke; G F Carle; M V Olson
Journal:  Science       Date:  1987-05-15       Impact factor: 47.728

2.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors:  R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

3.  Alu polymerase chain reaction: a method for rapid isolation of human-specific sequences from complex DNA sources.

Authors:  D L Nelson; S A Ledbetter; L Corbo; M F Victoria; R Ramírez-Solis; T D Webster; D H Ledbetter; C T Caskey
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

4.  A model for the separation of large DNA molecules by crossed field gel electrophoresis.

Authors:  E M Southern; R Anand; W R Brown; D S Fletcher
Journal:  Nucleic Acids Res       Date:  1987-08-11       Impact factor: 16.971

5.  Studies on polynucleotides. XCVI. Repair replications of short synthetic DNA's as catalyzed by DNA polymerases.

Authors:  K Kleppe; E Ohtsuka; R Kleppe; I Molineux; H G Khorana
Journal:  J Mol Biol       Date:  1971-03-14       Impact factor: 5.469

6.  Directional cloning of DNA fragments at a large distance from an initial probe: a circularization method.

Authors:  F S Collins; S M Weissman
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

7.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

8.  Removal of repeated sequences from hybridisation probes.

Authors:  P G Sealey; P A Whittaker; E M Southern
Journal:  Nucleic Acids Res       Date:  1985-03-25       Impact factor: 16.971

9.  Transformation of yeast spheroplasts without cell fusion.

Authors:  P M Burgers; K J Percival
Journal:  Anal Biochem       Date:  1987-06       Impact factor: 3.365

10.  Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors:  D C Schwartz; C R Cantor
Journal:  Cell       Date:  1984-05       Impact factor: 41.582
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  51 in total

1.  High-resolution landmark framework for the sequence-ready mapping of Xq23-q26.1.

Authors:  H E Steingruber; A Dunham; A J Coffey; S M Clegg; G R Howell; G L Maslen; C E Scott; R Gwilliam; P J Hunt; E C Sotheran; E J Huckle; S E Hunt; P Dhami; C Soderlund; M A Leversha; D R Bentley; M T Ross
Journal:  Genome Res       Date:  1999-08       Impact factor: 9.043

2.  Methods for intense aeration, growth, storage, and replication of bacterial strains in microtiter plates.

Authors:  W A Duetz; L Rüedi; R Hermann; K O'Connor; J Büchs; B Witholt
Journal:  Appl Environ Microbiol       Date:  2000-06       Impact factor: 4.792

3.  Integration of image analysis and robotics into a fully automated colony picking and plate handling system.

Authors:  P Jones; A Watson; M Davies; S Stubbings
Journal:  Nucleic Acids Res       Date:  1992-09-11       Impact factor: 16.971

4.  A YAC contig across the fragile X site defines the region of fragility.

Authors:  M C Hirst; K Rack; Y Nakahori; A Roche; M V Bell; G Flynn; Z Christadoulou; R N MacKinnon; M Francis; A J Littler
Journal:  Nucleic Acids Res       Date:  1991-06-25       Impact factor: 16.971

5.  Construction and characterisation of a yeast artificial chromosome library containing three haploid maize genome equivalents.

Authors:  K J Edwards; H Thompson; D Edwards; A de Saizieu; C Sparks; J A Thompson; A J Greenland; M Eyers; W Schuch
Journal:  Plant Mol Biol       Date:  1992-05       Impact factor: 4.076

Review 6.  Long-range walking techniques in positional cloning strategies.

Authors:  L Stubbs
Journal:  Mamm Genome       Date:  1992       Impact factor: 2.957

7.  Construction of a library enriched for human chromosome 11 and Xp YAC recombinants.

Authors:  B Arveiler; I Murray; B Stevenson; D J Porteous
Journal:  Mamm Genome       Date:  1991       Impact factor: 2.957

8.  Two sequence-tagged sites defining the ends of a 380 kb YAC clone from 19q13.

Authors:  R Butler; J H Riley; D J Ogilvie; R Anand; J Buxton; J Davies; K Johnson; A F Markham
Journal:  Nucleic Acids Res       Date:  1991-09-11       Impact factor: 16.971

9.  Construction of a YAC library from a Beta vulgaris fragment addition and isolation of a major satellite DNA cluster linked to the beet cyst nematode resistance locus Hs1 (pat-1.).

Authors:  R M Klein-Lankhorst; E M Salentijn; W G Dirkse; M Arens-de Reuver; W J Stiekema
Journal:  Theor Appl Genet       Date:  1994-10       Impact factor: 5.699

10.  Characterization of the human beta-catenin gene.

Authors:  A L Norris; A Bailey; J Askham; A Whitehouse; P M Clissold; A F Markham; D M Meredith
Journal:  Mamm Genome       Date:  1996-02       Impact factor: 2.957
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