Literature DB >> 1408762

Integration of image analysis and robotics into a fully automated colony picking and plate handling system.

P Jones1, A Watson, M Davies, S Stubbings.   

Abstract

We describe here the integration of image analysis and robotics to produce a fully automated colony picking/plate handling system. Biological tests were performed to verify its performance in terms of sterilisation and accuracy of picking. The machine was then used by a single operative to pick a 36,000 clone cDNA library in approximately 42 hrs over 5 days.

Mesh:

Year:  1992        PMID: 1408762      PMCID: PMC334190          DOI: 10.1093/nar/20.17.4599

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN: 0305-1048            Impact factor:   16.971


  15 in total

1.  Construction, arraying, and high-density screening of large insert libraries of human chromosomes X and 21: their potential use as reference libraries.

Authors:  D Nizetić; G Zehetner; A P Monaco; L Gellen; B D Young; H Lehrach
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-15       Impact factor: 11.205

2.  Preparation of clone libraries in yeast artificial-chromosome vectors.

Authors:  D T Burke; M V Olson
Journal:  Methods Enzymol       Date:  1991       Impact factor: 1.600

3.  Human genome analysis system.

Authors:  I Endo; E Soeda; Y Murakami; K Nishi
Journal:  Nature       Date:  1991-07-04       Impact factor: 49.962

4.  Yeast artificial chromosome libraries containing large inserts from mouse and human DNA.

Authors:  Z Larin; A P Monaco; H Lehrach
Journal:  Proc Natl Acad Sci U S A       Date:  1991-05-15       Impact factor: 11.205

5.  The development and application of automated gridding for efficient screening of yeast and bacterial ordered libraries.

Authors:  D R Bentley; C Todd; J Collins; J Holland; I Dunham; S Hassock; A Bankier; F Giannelli
Journal:  Genomics       Date:  1992-03       Impact factor: 5.736

6.  Application of robotics and image processing to automated colony picking and arraying.

Authors:  D C Uber; J M Jaklevic; E H Theil; A Lishanskaya; M R McNeely
Journal:  Biotechniques       Date:  1991-11       Impact factor: 1.993

7.  A 3.5 genome equivalent multi access YAC library: construction, characterisation, screening and storage.

Authors:  R Anand; J H Riley; R Butler; J C Smith; A F Markham
Journal:  Nucleic Acids Res       Date:  1990-04-25       Impact factor: 16.971

8.  Semiautomated preparation of DNA templates for large-scale sequencing projects.

Authors:  V Smith; C M Brown; A T Bankier; B G Barrell
Journal:  DNA Seq       Date:  1990

9.  Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors:  W D Benton; R W Davis
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

10.  A turning point in cancer research: sequencing the human genome.

Authors:  R Dulbecco
Journal:  Science       Date:  1986-03-07       Impact factor: 47.728

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  4 in total

1.  Efficient division and sampling of cell colonies using microcup arrays.

Authors:  Jeng-Hao Pai; Kimberly Kluckman; Dale O Cowley; Donna M Bortner; Christopher E Sims; Nancy L Allbritton
Journal:  Analyst       Date:  2012-10-25       Impact factor: 4.616

2.  Novel concept microarray enabling PCR and multistep reactions through pipette-free aperture-to-aperture parallel transfer.

Authors:  Yasunori Kinoshita; Takahiro Tayama; Koichiro Kitamura; M Salimullah; Hidekazu Uchida; Miho Suzuki; Yuzuru Husimi; Koichi Nishigaki
Journal:  BMC Biotechnol       Date:  2010-10-06       Impact factor: 2.563

3.  Collaborative Robot Precision Task in Medical Microbiology Laboratory.

Authors:  Aljaz Baumkircher; Katja Seme; Marko Munih; Matjaž Mihelj
Journal:  Sensors (Basel)       Date:  2022-04-08       Impact factor: 3.576

4.  Novel method for high-throughput colony PCR screening in nanoliter-reactors.

Authors:  Marcel Walser; Rene Pellaux; Andreas Meyer; Matthias Bechtold; Herve Vanderschuren; Richard Reinhardt; Joseph Magyar; Sven Panke; Martin Held
Journal:  Nucleic Acids Res       Date:  2009-03-12       Impact factor: 16.971

  4 in total

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