Literature DB >> 3310730

Transformation of yeast spheroplasts without cell fusion.

P M Burgers1, K J Percival.   

Abstract

The efficiency of genetic transformation of Saccharomyces cerevisiae spheroplasts has been increased 10- to 100-fold over previously published procedures. Optimal transformation frequencies for single-stranded and double-stranded replicating plasmids are 2 X 10(7) and 5 X 10(6) transformants/microgram, respectively. At saturating DNA concentrations, 12 and 3%, respectively, of the viable spheroplasts contain plasmid DNA. The percentage of transformants that have undergone nuclear fusion varies from 0.1 to 3%, indicating that fusion is not required for the uptake of DNA by yeast spheroplasts.

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Year:  1987        PMID： 3310730     DOI： 10.1016/0003-2697(87)90240-5

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  104 in total

1.  Construction of a yeast artificial chromosome library of tomato and identification of cloned segments linked to two disease resistance loci.

Authors:  G B Martin; M W Ganal; S D Tanksley
Journal:  Mol Gen Genet       Date:  1992-05

2.  Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts.

Authors:  J M Rossi; D T Burke; J C Leung; D S Koos; H Chen; S M Tilghman
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-15       Impact factor: 11.205

3.  Centromeric DNA sequences in the pathogenic yeast Candida albicans are all different and unique.

Authors:  Kaustuv Sanyal; Mary Baum; John Carbon
Journal:  Proc Natl Acad Sci U S A       Date:  2004-07-22       Impact factor: 11.205

4.  Amplification of large artificial chromosomes.

Authors:  D R Smith; A P Smyth; D T Moir
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

5.  Streamlined approach to creating yeast artificial chromosome libraries from specialized cell sources.

Authors:  J M Feingold; S D Ogden; C T Denny
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

6.  Random-breakage mapping, a rapid method for physically locating an internal sequence with respect to the ends of a DNA molecule.

Authors:  J C Game; M Bell; J S King; R K Mortimer
Journal:  Nucleic Acids Res       Date:  1990-08-11       Impact factor: 16.971

7.  Alu-primed polymerase chain reaction for regional assignment of 110 yeast artificial chromosome clones from the human X chromosome: identification of clones associated with a disease locus.

Authors:  D L Nelson; A Ballabio; M F Victoria; M Pieretti; R D Bies; R A Gibbs; J A Maley; A C Chinault; T D Webster; C T Caskey
Journal:  Proc Natl Acad Sci U S A       Date:  1991-07-15       Impact factor: 11.205

8.  Cloning and molecular analysis of two different ILV5 genes from a brewing strain of Saccharomyces cerevisiae.

Authors:  Q Xie; A Jiménez
Journal:  Curr Genet       Date:  1994 Nov-Dec       Impact factor: 3.886

9.  Physical mapping of the Myxococcus xanthus genome by random cloning in yeast artificial chromosomes.

Authors:  A Kuspa; D Vollrath; Y Cheng; D Kaiser
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205

10.  Introduction of YACs into intact yeast cells by a procedure which shows low levels of recombinagenicity and co-transformation.

Authors:  S M Heale; L I Stateva; S G Oliver
Journal:  Nucleic Acids Res       Date:  1994-11-25       Impact factor: 16.971
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