Literature DB >> 21840396

Simple screening method for improving membrane protein thermostability.

Romina Mancusso1, Nathan K Karpowich, Bryan K Czyzewski, Da-Neng Wang.   

Abstract

Biochemical and biophysical analysis on integral membrane proteins often requires monodisperse and stable protein samples. Here we describe a method to characterize protein thermostability by measuring its melting temperature in detergent using analytical size-exclusion chromatography. This quantitative method can be used to screen for compounds and conditions that stabilize the protein. With this technique we were able to assess and improve the thermostability of several membrane proteins. These conditions were in turn used to assist purification, to identify protein ligand and to improve crystal quality.
Copyright © 2011 Elsevier Inc. All rights reserved.

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Year:  2011        PMID： 21840396      PMCID： PMC3220791          DOI： 10.1016/j.ymeth.2011.07.008

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  62 in total

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