OBJECTIVES: Collagen disorganization is one of the main degradation patterns found in unsuccessful adhesive restorations. The hypothesis of this study was that pretreatment using natural collagen cross-linking agents rich in proanthocyanidin (PA) would improve mechanical properties and stability over time of the dentin collagen and, thus, confer a more resistant and lasting substrate for adhesive restorations. METHODS: PA-based extracts, from grape seed (GSE), cocoa seed (CSE), cranberry (CRE), cinnamon (CNE) and açaí berry (ACE) were applied over the demineralized dentin. The apparent elastic modulus (E) of the treated dentin collagen was analyzed over a 12 month period. Specimens were immersed in the respective solution and E values were obtained by a micro-flexural test at baseline, 10, 30, 60, 120 and 240 min. Samples were stored in artificial saliva and re-tested after 3, 6 and 12 months. Data was analyzed using ANOVA and Tukey test. RESULTS: GSE and CSE extracts showed a time-dependent effect and were able to improve [240 min (MPa): GSE = 108.96 ± 56.08;CSE = 59.21 ± 24.87] and stabilize the E of the organic matrix [12 months (MPa): GSE = 40.91 ± 19.69;CSE = 42.11 ± 13.46]. CRE and CNE extracts were able to maintain the E of collagen matrices constant over 12 months [CRE = 11.17 ± 7.22;CNE = 9,96 ± 6.11;MPa]. ACE (2.64 ± 1.22 MPa) and control groups immersed in neat distilled water (1.37 ± 0.69 MPa) and ethanol-water (0.95 ± 0.33 MPa) showed no effect over dentin organic matrix and enable their degradation and reduction of mechanical properties. SIGNIFICANCE: Some PA-based extracts were capable of improving and stabilizing collagen matrices through exogenous cross-links induction.
OBJECTIVES: Collagen disorganization is one of the main degradation patterns found in unsuccessful adhesive restorations. The hypothesis of this study was that pretreatment using natural collagen cross-linking agents rich in proanthocyanidin (PA) would improve mechanical properties and stability over time of the dentin collagen and, thus, confer a more resistant and lasting substrate for adhesive restorations. METHODS:PA-based extracts, from grape seed (GSE), cocoa seed (CSE), cranberry (CRE), cinnamon (CNE) and açaí berry (ACE) were applied over the demineralized dentin. The apparent elastic modulus (E) of the treated dentin collagen was analyzed over a 12 month period. Specimens were immersed in the respective solution and E values were obtained by a micro-flexural test at baseline, 10, 30, 60, 120 and 240 min. Samples were stored in artificial saliva and re-tested after 3, 6 and 12 months. Data was analyzed using ANOVA and Tukey test. RESULTS: GSE and CSE extracts showed a time-dependent effect and were able to improve [240 min (MPa): GSE = 108.96 ± 56.08;CSE = 59.21 ± 24.87] and stabilize the E of the organic matrix [12 months (MPa): GSE = 40.91 ± 19.69;CSE = 42.11 ± 13.46]. CRE and CNE extracts were able to maintain the E of collagen matrices constant over 12 months [CRE = 11.17 ± 7.22;CNE = 9,96 ± 6.11;MPa]. ACE (2.64 ± 1.22 MPa) and control groups immersed in neat distilled water (1.37 ± 0.69 MPa) and ethanol-water (0.95 ± 0.33 MPa) showed no effect over dentin organic matrix and enable their degradation and reduction of mechanical properties. SIGNIFICANCE: Some PA-based extracts were capable of improving and stabilizing collagen matrices through exogenous cross-links induction.
Authors: Rasika S Phansalkar; Joo-Won Nam; Shao-Nong Chen; James B McAlpine; José G Napolitano; Ariene Leme; Cristina M P Vidal; Thaiane Aguiar; Ana K Bedran-Russo; Guido F Pauli Journal: Fitoterapia Date: 2014-12-24 Impact factor: 2.882
Authors: Valeria B Gotti; Américo B Correr; Steven H Lewis; Victor P Feitosa; Lourenço Correr-Sobrinho; Jeffrey W Stansbury Journal: Dent Mater Date: 2016-09-21 Impact factor: 5.304
Authors: T R Aguiar; C M P Vidal; R S Phansalkar; I Todorova; J G Napolitano; J B McAlpine; S N Chen; G F Pauli; A K Bedran-Russo Journal: J Dent Res Date: 2014-02-26 Impact factor: 6.116
Authors: Cristina M P Vidal; Thaiane R Aguiar; Rasika Phansalkar; James B McAlpine; José G Napolitano; Shao-Nong Chen; Larissa S N Araújo; Guido F Pauli; Ana Bedran-Russo Journal: Acta Biomater Date: 2014-04-08 Impact factor: 8.947
Authors: Rasika S Phansalkar; Joo-Won Nam; Shao-Nong Chen; James B McAlpine; Ariene A Leme; Berdan Aydin; Ana-Karina Bedran-Russo; Guido F Pauli Journal: J Chromatogr A Date: 2017-12-21 Impact factor: 4.759
Authors: Ana K Bedran-Russo; Guido F Pauli; Shao-Nong Chen; James McAlpine; Carina S Castellan; Rasika S Phansalkar; Thaiane R Aguiar; Cristina M P Vidal; José G Napotilano; Joo-Won Nam; Ariene A Leme Journal: Dent Mater Date: 2013-12-03 Impact factor: 5.304