Literature DB >> 21725299

Megapixel digital PCR.

Kevin A Heyries1, Carolina Tropini, Michael Vaninsberghe, Callum Doolin, Oleh I Petriv, Anupam Singhal, Kaston Leung, Curtis B Hughesman, Carl L Hansen.   

Abstract

We present a microfluidic 'megapixel' digital PCR device that uses surface tension-based sample partitioning and dehydration control to enable high-fidelity single DNA molecule amplification in 1,000,000 reactors of picoliter volume with densities up to 440,000 reactors cm(-2). This device achieves a dynamic range of 10(7), single-nucleotide-variant detection below one copy per 100,000 wild-type sequences and the discrimination of a 1% difference in chromosome copy number.

Entities:  

Mesh：

Year:  2011        PMID： 21725299     DOI： 10.1038/nmeth.1640

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  15 in total

1.  Digital PCR.

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2.  Microfluidic large-scale integration.

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3.  BEAMing up for detection and quantification of rare sequence variants.

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4.  Frequent detection of the JAK2 V617F mutation in bone marrow core biopsy specimens from chronic myeloproliferative disorders using the TaqMan polymerase chain reaction single nucleotide polymorphism genotyping assay: a retrospective study with pathologic correlations.

Authors:  Marina Bousquet; Sophie Le Guellec; Cathy Quelen; Françoise Rigal-Huguet; Georges Delsol; Pierre Brousset
Journal:  Hum Pathol       Date:  2006-07-26       Impact factor: 3.466

5.  Fabricating large arrays of microwells with arbitrary dimensions and filling them using discontinuous dewetting.

Authors:  R J Jackman; D C Duffy; E Ostuni; N D Willmore; G M Whitesides
Journal:  Anal Chem       Date:  1998-06-01       Impact factor: 6.986

6.  Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase.

Authors:  K R Tindall; T A Kunkel
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7.  Digital PCR on a SlipChip.

Authors:  Feng Shen; Wenbin Du; Jason E Kreutz; Alice Fok; Rustem F Ismagilov
Journal:  Lab Chip       Date:  2010-07-01       Impact factor: 6.799

8.  Self-digitization of sample volumes.

Authors:  Dawn E Cohen; Thomas Schneider; Michelle Wang; Daniel T Chiu
Journal:  Anal Chem       Date:  2010-07-01       Impact factor: 6.986

9.  Circulating mutant DNA to assess tumor dynamics.

Authors:  Frank Diehl; Kerstin Schmidt; Michael A Choti; Katharine Romans; Steven Goodman; Meng Li; Katherine Thornton; Nishant Agrawal; Lori Sokoll; Steve A Szabo; Kenneth W Kinzler; Bert Vogelstein; Luis A Diaz
Journal:  Nat Med       Date:  2007-07-31       Impact factor: 53.440

10.  High-throughput quantitative polymerase chain reaction in picoliter droplets.

Authors:  Margaret Macris Kiss; Lori Ortoleva-Donnelly; N Reginald Beer; Jason Warner; Christopher G Bailey; Bill W Colston; Jonathon M Rothberg; Darren R Link; John H Leamon
Journal:  Anal Chem       Date:  2008-12-01       Impact factor: 6.986
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  65 in total

1.  Simple Bulk Readout of Digital Nucleic Acid Quantification Assays.

Authors:  Leanna S Morinishi; Paul Blainey
Journal:  J Vis Exp       Date:  2015-09-24       Impact factor: 1.355

2.  Another transistor-based revolution: on-chip qPCR.

Authors:  Carlotta Guiducci; Fabio M Spiga
Journal:  Nat Methods       Date:  2013-07       Impact factor: 28.547

3.  Oil-sealed femtoliter fiber-optic arrays for single molecule analysis.

Authors:  Huaibin Zhang; Shuai Nie; Candice M Etson; Raymond M Wang; David R Walt
Journal:  Lab Chip       Date:  2012-02-06       Impact factor: 6.799

4.  Simultaneous DNA amplification and detection using a pH-sensing semiconductor system.

Authors:  Christofer Toumazou; Leila M Shepherd; Samuel C Reed; Ginny I Chen; Alpesh Patel; David M Garner; Chan-Ju A Wang; Chung-Pei Ou; Krishna Amin-Desai; Panteleimon Athanasiou; Hua Bai; Ines M Q Brizido; Benjamin Caldwell; Daniel Coomber-Alford; Pantelis Georgiou; Karen S Jordan; John C Joyce; Maurizio La Mura; Daniel Morley; Sreekala Sathyavruthan; Sara Temelso; Risha E Thomas; Linglan Zhang
Journal:  Nat Methods       Date:  2013-06-09       Impact factor: 28.547

Review 5.  Role of quantitative and qualitative characteristics of free circulating DNA in the management of patients with non-small cell lung cancer.

Authors:  Paola Ulivi; Rosella Silvestrini
Journal:  Cell Oncol (Dordr)       Date:  2013-11-01       Impact factor: 6.730

6.  Single cell digital polymerase chain reaction on self-priming compartmentalization chip.

Authors:  Qiangyuan Zhu; Lin Qiu; Yanan Xu; Guang Li; Ying Mu
Journal:  Biomicrofluidics       Date:  2017-01-31       Impact factor: 2.800

Review 7.  Micro total analysis systems: fundamental advances and applications in the laboratory, clinic, and field.

Authors:  Michelle L Kovarik; Douglas M Ornoff; Adam T Melvin; Nicholas C Dobes; Yuli Wang; Alexandra J Dickinson; Philip C Gach; Pavak K Shah; Nancy L Allbritton
Journal:  Anal Chem       Date:  2012-12-04       Impact factor: 6.986

8.  Chip in a lab: Microfluidics for next generation life science research.

Authors:  Aaron M Streets; Yanyi Huang
Journal:  Biomicrofluidics       Date:  2013-01-31       Impact factor: 2.800

9.  Droplet microfluidics driven by gradients of confinement.

Authors:  Rémi Dangla; S Cagri Kayi; Charles N Baroud
Journal:  Proc Natl Acad Sci U S A       Date:  2013-01-02       Impact factor: 11.205

10.  Genomic DNA extraction from cells by electroporation on an integrated microfluidic platform.

Authors:  Tao Geng; Ning Bao; Nammalwar Sriranganathanw; Liwu Li; Chang Lu
Journal:  Anal Chem       Date:  2012-10-23       Impact factor: 6.986
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