| Literature DB >> 21700836 |
Andrew J Wood1, Te-Wen Lo, Bryan Zeitler, Catherine S Pickle, Edward J Ralston, Andrew H Lee, Rainier Amora, Jeffrey C Miller, Elo Leung, Xiangdong Meng, Lei Zhang, Edward J Rebar, Philip D Gregory, Fyodor D Urnov, Barbara J Meyer.
Abstract
Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with sequenced genomes. We established a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species.Entities:
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Year: 2011 PMID： 21700836 PMCID： PMC3489282 DOI： 10.1126/science.1207773
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728