Literature DB >> 21677066

Prevalence and genetic relatedness of methicillin-susceptible Staphylococcus aureus isolates detected by the Xpert MRSA nasal assay.

Sophie S Arbefeville1, Kunyan Zhang, Jennifer S Kroeger, Wanita J Howard, Daniel J Diekema, Sandra S Richter.   

Abstract

Methicillin-susceptible Staphylococcus aureus (MSSA) isolates lacking mecA yet testing positive on the Xpert MRSA assay were recovered from culture for 7.7% of 248 Xpert-positive nasal samples. These "false-positive" Xpert results may be attributed to staphylococcal cassette chromosome (SCC) elements without the mecA gene. Pulsed-field gel electrophoresis (PFGE) analysis revealed a diverse population of MSSA strains.

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Year:  2011        PMID： 21677066      PMCID： PMC3147776          DOI： 10.1128/JCM.00046-11

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  19 in total

1.  Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus.

Authors:  Kunyan Zhang; Jo-Ann McClure; Sameer Elsayed; Thomas Louie; John M Conly
Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

2.  Performance of the BD GeneOhm methicillin-resistant Staphylococcus aureus test before and during high-volume clinical use.

Authors:  Suzanne M Paule; Donna M Hacek; Bridget Kufner; Karine Truchon; Richard B Thomson; Karen L Kaul; Ari Robicsek; Lance R Peterson
Journal:  J Clin Microbiol       Date:  2007-07-11       Impact factor: 5.948

3.  Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus.

Authors:  T Ito; Y Katayama; K Asada; N Mori; K Tsutsumimoto; C Tiensasitorn; K Hiramatsu
Journal:  Antimicrob Agents Chemother       Date:  2001-05       Impact factor: 5.191

4.  Partial excision of the chromosomal cassette containing the methicillin resistance determinant results in methicillin-susceptible Staphylococcus aureus.

Authors:  Pierre-Yves Donnio; Duarte C Oliveira; Nuno A Faria; Nathalie Wilhelm; Alain Le Coustumier; Herminia de Lencastre
Journal:  J Clin Microbiol       Date:  2005-08       Impact factor: 5.948

5.  Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec, ccr, and major differences in junkyard regions.

Authors:  Yoko Kondo; Teruyo Ito; Xiao Xue Ma; Shinya Watanabe; Barry N Kreiswirth; Jerome Etienne; Keiichi Hiramatsu
Journal:  Antimicrob Agents Chemother       Date:  2006-10-16       Impact factor: 5.191

Review 6.  Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing.

Authors:  F C Tenover; R D Arbeit; R V Goering; P A Mickelsen; B E Murray; D H Persing; B Swaminathan
Journal:  J Clin Microbiol       Date:  1995-09       Impact factor: 5.948

7.  Evaluation of the IDI-MRSA assay for detection of methicillin-resistant staphylococcus aureus from nasal and rectal specimens pooled in a selective broth.

Authors:  M Desjardins; Christiane Guibord; B Lalonde; B Toye; K Ramotar
Journal:  J Clin Microbiol       Date:  2006-04       Impact factor: 5.948

8.  New quadriplex PCR assay for detection of methicillin and mupirocin resistance and simultaneous discrimination of Staphylococcus aureus from coagulase-negative staphylococci.

Authors:  Kunyan Zhang; Jennifer Sparling; Barbara L Chow; Sameer Elsayed; Zafar Hussain; Deirdre L Church; Daniel B Gregson; Thomas Louie; John M Conly
Journal:  J Clin Microbiol       Date:  2004-11       Impact factor: 5.948

9.  Characterization of Staphylococcus aureus isolates with a partial or complete absence of staphylococcal cassette chromosome elements.

Authors:  Henry Wong; Lisa Louie; Reggie Y C Lo; Andrew E Simor
Journal:  J Clin Microbiol       Date:  2010-07-28       Impact factor: 5.948

10.  New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci.

Authors:  A Huletsky; R Giroux; V Rossbach; M Gagnon; M Vaillancourt; M Bernier; F Gagnon; K Truchon; M Bastien; F J Picard; A van Belkum; M Ouellette; P H Roy; M G Bergeron
Journal:  J Clin Microbiol       Date:  2004-05       Impact factor: 5.948
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  14 in total

1.  Cepheid Xpert MRSA cycle threshold in discordant colonization results and as a quantitative measure of nasal colonization burden.

Authors:  Edward Stenehjem; David Rimland; Emily K Crispell; Cortney Stafford; Robert Gaynes; Sarah W Satola
Journal:  J Clin Microbiol       Date:  2012-03-21       Impact factor: 5.948

2.  Rapid confirmation of suspected methicillin-resistant Staphylococcus aureus colonies on chromogenic agars by a new commercial PCR assay, the GenomEra MRSA/SA Diagnose.

Authors:  J J Hirvonen; M Nevalainen; P Tissari; S Salmenlinna; K Rantakokko-Jalava; S-S Kaukoranta
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2012-01-26       Impact factor: 3.267

3.  High frequency of occupied attB regions in Norwegian Staphylococcus aureus isolates supports a two-step MRSA screening algorithm.

Authors:  H S Tunsjø; S Kalyanasundaram; M M Worren; T M Leegaard; A E F Moen
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2016-09-09       Impact factor: 3.267

4.  Evaluation of FilmArray and Verigene systems for rapid identification of positive blood cultures.

Authors:  M M Bhatti; S Boonlayangoor; K G Beavis; V Tesic
Journal:  J Clin Microbiol       Date:  2014-07-16       Impact factor: 5.948

5.  Characterization of the staphylococcal cassette chromosome mec insertion site in 108 isolates lacking the mecA gene and identified as methicillin-resistant Staphylococcus aureus by the Xpert MRSA assay.

Authors:  M Stojanov; D S Blanc
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2014-06-07       Impact factor: 3.267

6.  Evaluation of BD Max StaphSR and BD Max MRSAXT Assays Using ESwab-Collected Specimens.

Authors:  Suzane Silbert; Carly Kubasek; Faris Galambo; Elaine Vendrone; Raymond Widen
Journal:  J Clin Microbiol       Date:  2015-05-27       Impact factor: 5.948

7.  Detection of mecA- and mecC-Positive Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates by the New Xpert MRSA Gen 3 PCR Assay.

Authors:  Karsten Becker; Olivier Denis; Sandrine Roisin; Alexander Mellmann; Evgeny A Idelevich; Dennis Knaack; Sarah van Alen; André Kriegeskorte; Robin Köck; Frieder Schaumburg; Georg Peters; Britta Ballhausen
Journal:  J Clin Microbiol       Date:  2015-10-21       Impact factor: 5.948

8.  Detection of methicillin-resistant Staphylococcus aureus by a duplex droplet digital PCR assay.

Authors:  Kashonda Kelley; Angela Cosman; Phillip Belgrader; Brenda Chapman; Donna C Sullivan
Journal:  J Clin Microbiol       Date:  2013-04-17       Impact factor: 5.948

9.  Molecular epidemiology of methicillin-resistant Staphylococcus aureus isolates from patients newly identified as nasal carriers.

Authors:  Susan K Weir; Julia Fram; Gretchen Berg; Dima Kabbani; Judith Strymish; Melody Tang; Dianne Fitzsimmons; Stephen M Brecher; Kalpana Gupta
Journal:  J Clin Microbiol       Date:  2012-07-25       Impact factor: 5.948

10.  Contamination of environmental surfaces with Staphylococcus aureus in households with children infected with methicillin-resistant S aureus.

Authors:  Stephanie A Fritz; Patrick G Hogan; Lauren N Singh; Ryley M Thompson; Meghan A Wallace; Krista Whitney; Duha Al-Zubeidi; Carey-Ann D Burnham; Victoria J Fraser
Journal:  JAMA Pediatr       Date:  2014-11       Impact factor: 16.193
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