Literature DB >> 2167318

The histone H3/H4.N1 complex supplemented with histone H2A-H2B dimers and DNA topoisomerase I forms nucleosomes on circular DNA under physiological conditions.

K Zucker1, A Worcel.   

Abstract

We have fractionated the whole cell extract of Xenopus oocytes (oocyte S-150) and isolated the endogenous components required for DNA supercoiling and nucleosome formation. Histone H2B and the three oocyte-specific H2A proteins were purified as free histones. Histones H3 and H4 were purified 100-fold in a complex with the acidic protein N1. In the presence of DNA topoisomerase I or II, histone H3/H4.N1 complexes supercoil DNA in a reaction that is inhibited by Mg2+, and this inhibition is relieved by NTPs. The supercoiling reaction induced by H3/H4.N1 complexes is enhanced by free histone H2A-H2B dimers, which by themselves do not supercoil DNA. Nuclease digestions and protein analyses indicate that H3/H4.N1 complexes form subnucleosomal particles containing histones H3 and H4. Nucleosomes containing 146-base pair DNA and the four histones are formed when histones H2A and H2B complement the reaction.

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Year:  1990        PMID： 2167318

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  11 in total

1.  The protein encoded by the proto-oncogene DEK changes the topology of chromatin and reduces the efficiency of DNA replication in a chromatin-specific manner.

Authors:  V Alexiadis; T Waldmann; J Andersen; M Mann; R Knippers; C Gruss
Journal:  Genes Dev       Date:  2000-06-01       Impact factor: 11.361

2.  Analysis of histones in Xenopus laevis. I. A distinct index of enriched variants and modifications exists in each cell type and is remodeled during developmental transitions.

Authors:  David Shechter; Joshua J Nicklay; Raghu K Chitta; Jeffrey Shabanowitz; Donald F Hunt; C David Allis
Journal:  J Biol Chem       Date:  2008-10-28       Impact factor: 5.157

3.  Crystal structure and function of human nucleoplasmin (npm2): a histone chaperone in oocytes and embryos.

Authors:  Olga Platonova; Ildikó V Akey; James F Head; Christopher W Akey
Journal:  Biochemistry       Date:  2011-08-24       Impact factor: 3.162

4.  The binding of a Fos/Jun heterodimer can completely disrupt the structure of a nucleosome.

Authors:  K W Ng; P Ridgway; D R Cohen; D J Tremethick
Journal:  EMBO J       Date:  1997-04-15       Impact factor: 11.598

5.  Assembly of regularly spaced nucleosome arrays by Drosophila chromatin assembly factor 1 and a 56-kDa histone-binding protein.

Authors:  M Bulger; T Ito; R T Kamakaka; J T Kadonaga
Journal:  Proc Natl Acad Sci U S A       Date:  1995-12-05       Impact factor: 11.205

6.  Molecular cloning and functional characterization of a cDNA encoding nucleosome assembly protein 1 (NAP-1) from soybean.

Authors:  H W Yoon; M C Kim; S Y Lee; I Hwang; J D Bahk; J C Hong; Y Ishimi; M J Cho
Journal:  Mol Gen Genet       Date:  1995-12-15

7.  Stimulation of transcription factor binding and histone displacement by nucleosome assembly protein 1 and nucleoplasmin requires disruption of the histone octamer.

Authors:  P P Walter; T A Owen-Hughes; J Côté; J L Workman
Journal:  Mol Cell Biol       Date:  1995-11       Impact factor: 4.272

8.  Stepwise assembly of chromatin during DNA replication in vitro.

Authors:  S Smith; B Stillman
Journal:  EMBO J       Date:  1991-04       Impact factor: 11.598

9.  Disruption of the nucleosomes at the replication fork.

Authors:  C Gruss; J Wu; T Koller; J M Sogo
Journal:  EMBO J       Date:  1993-12       Impact factor: 11.598

10.  A histone-binding protein, nucleoplasmin, stimulates transcription factor binding to nucleosomes and factor-induced nucleosome disassembly.

Authors:  H Chen; B Li; J L Workman
Journal:  EMBO J       Date:  1994-01-15       Impact factor: 11.598
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