Literature DB >> 21652525

Successive and targeted DNA integrations in the Drosophila genome by Bxb1 and phiC31 integrases.

Juan Huang1, Pallavi Ghosh, Graham F Hatfull, Yang Hong.   

Abstract

At present ϕC31 is the only phage integrase system available for directionally regulated site-specific DNA integration in the Drosophila genome. Here we report that mycobacteriophage Bxb1 integrase also mediates targeted DNA integration in Drosophila with high specificity and efficiency. By alternately using Bxb1 and ϕC31, we were able to carry out multiple rounds of successive and targeted DNA integrations in our genomic engineering founder lines for the purpose of generating complex knock-in alleles.

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Year:  2011        PMID： 21652525      PMCID： PMC3176133          DOI： 10.1534/genetics.111.129247

Source DB:  PubMed          Journal:  Genetics        ISSN： 0016-6731            Impact factor:   4.562


  20 in total

1.  Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis.

Authors:  T Lee; L Luo
Journal:  Neuron       Date:  1999-03       Impact factor: 17.173

Review 2.  GM maize from site-specific recombination technology, what next?

Authors:  David W Ow
Journal:  Curr Opin Biotechnol       Date:  2007-03-13       Impact factor: 9.740

3.  Conditional mutagenesis in Drosophila.

Authors:  Ching Man Choi; Sven Vilain; Marion Langen; Sofie Van Kelst; Natalie De Geest; Jiekun Yan; Patrik Verstreken; Bassem A Hassan
Journal:  Science       Date:  2009-04-03       Impact factor: 47.728

4.  A powerful method combining homologous recombination and site-specific recombination for targeted mutagenesis in Drosophila.

Authors:  Guanjun Gao; Conor McMahon; Jie Chen; Yikang S Rong
Journal:  Proc Natl Acad Sci U S A       Date:  2008-09-04       Impact factor: 11.205

5.  Efficient ends-out gene targeting in Drosophila.

Authors:  Juan Huang; Wenke Zhou; Annie M Watson; Yuh-Nung Jan; Yang Hong
Journal:  Genetics       Date:  2008-08-30       Impact factor: 4.562

6.  Efficient site-specific integration in Plasmodium falciparum chromosomes mediated by mycobacteriophage Bxb1 integrase.

Authors:  Louis J Nkrumah; Rebecca A Muhle; Pedro A Moura; Pallavi Ghosh; Graham F Hatfull; William R Jacobs; David A Fidock
Journal:  Nat Methods       Date:  2006-08       Impact factor: 28.547

7.  An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases.

Authors:  Johannes Bischof; Robert K Maeda; Monika Hediger; François Karch; Konrad Basler
Journal:  Proc Natl Acad Sci U S A       Date:  2007-02-22       Impact factor: 11.205

8.  Insulating DNA directs ubiquitous transcription of the Drosophila melanogaster alpha 1-tubulin gene.

Authors:  K H O'Donnell; C T Chen; P C Wensink
Journal:  Mol Cell Biol       Date:  1994-09       Impact factor: 4.272

9.  Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster.

Authors:  Jian-Quan Ni; Michele Markstein; Richard Binari; Barret Pfeiffer; Lu-Ping Liu; Christians Villalta; Matthew Booker; Lizabeth Perkins; Norbert Perrimon
Journal:  Nat Methods       Date:  2007-12-16       Impact factor: 28.547

10.  Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes.

Authors:  Michele Markstein; Chrysoula Pitsouli; Christians Villalta; Susan E Celniker; Norbert Perrimon
Journal:  Nat Genet       Date:  2008-03-02       Impact factor: 38.330
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  17 in total

1.  An enhanced gene targeting toolkit for Drosophila: Golic+.

Authors:  Hui-Min Chen; Yaling Huang; Barret D Pfeiffer; Xiaohao Yao; Tzumin Lee
Journal:  Genetics       Date:  2015-01-02       Impact factor: 4.562

2.  Parallel Genomic Engineering of Two Drosophila Genes Using Orthogonal attB/attP Sites.

Authors:  Beatriz Blanco-Redondo; Tobias Langenhan
Journal:  G3 (Bethesda)       Date:  2018-08-30       Impact factor: 3.154

3.  Bxb1 phage recombinase assists genome engineering in Drosophila melanogaster.

Authors:  Roumen Voutev; Richard S Mann
Journal:  Biotechniques       Date:  2017-01-01       Impact factor: 1.993

4.  Molecular Genetics of Mycobacteriophages.

Authors:  Graham F Hatfull
Journal:  Microbiol Spectr       Date:  2014-03-07

5.  Cross-talk between diverse serine integrases.

Authors:  Shweta Singh; Kate Rockenbach; Rebekah M Dedrick; Andrew P VanDemark; Graham F Hatfull
Journal:  J Mol Biol       Date:  2013-10-22       Impact factor: 5.469

Review 6.  Genome engineering: Drosophila melanogaster and beyond.

Authors:  Koen J T Venken; Alejandro Sarrion-Perdigones; Paul J Vandeventer; Nicholas S Abel; Audrey E Christiansen; Kristi L Hoffman
Journal:  Wiley Interdiscip Rev Dev Biol       Date:  2015-10-08       Impact factor: 5.814

7.  Genetic manipulation of genes and cells in the nervous system of the fruit fly.

Authors:  Koen J T Venken; Julie H Simpson; Hugo J Bellen
Journal:  Neuron       Date:  2011-10-20       Impact factor: 17.173

8.  The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis.

Authors:  James G Thomson; Ronald Chan; Jamison Smith; Roger Thilmony; Yuan-Yeu Yau; Yueju Wang; David W Ow
Journal:  BMC Biotechnol       Date:  2012-03-21       Impact factor: 2.563

9.  Attachment site selection and identity in Bxb1 serine integrase-mediated site-specific recombination.

Authors:  Shweta Singh; Pallavi Ghosh; Graham F Hatfull
Journal:  PLoS Genet       Date:  2013-05-02       Impact factor: 5.917

10.  Vika/vox, a novel efficient and specific Cre/loxP-like site-specific recombination system.

Authors:  Madina Karimova; Josephine Abi-Ghanem; Nicolas Berger; Vineeth Surendranath; Maria Teresa Pisabarro; Frank Buchholz
Journal:  Nucleic Acids Res       Date:  2012-11-09       Impact factor: 16.971
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