| Literature DB >> 21642977 |
Jérôme Lecoq1, Alexandre Parpaleix, Emmanuel Roussakis, Mathieu Ducros, Yannick Goulam Houssen, Sergei A Vinogradov, Serge Charpak.
Abstract
Uncovering principles that regulate energy metabolism in the brain requires mapping of partial pressure of oxygen (PO(2)) and blood flow with high spatial and temporal resolution. Using two-photon phosphorescence lifetime microscopy (2PLM) and the oxygen probe PtP-C343, we show that PO(2) can be accurately measured in the brain at depths up to 300 μm with micron-scale resolution. In addition, 2PLM allowed simultaneous measurements of blood flow and of PO(2) in capillaries with less than one-second temporal resolution. Using this approach, we detected erythrocyte-associated transients (EATs) in oxygen in the rat olfactory bulb and showed the existence of diffusion-based arterio-venous shunts. Sensory stimulation evoked functional hyperemia, accompanied by an increase in PO(2) in capillaries and by a biphasic PO(2) response in the neuropil, consisting of an 'initial dip' and a rebound. 2PLM of PO(2) opens new avenues for studies of brain metabolism and blood flow regulation.Entities:
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Year: 2011 PMID： 21642977 PMCID： PMC3291110 DOI： 10.1038/nm.2394
Source DB: PubMed Journal: Nat Med ISSN： 1078-8956 Impact factor: 53.440