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Literature DB >> 21574560

An ENDOR and HYSCORE investigation of a reaction intermediate in IspG (GcpE) catalysis.

Weixue Wang¹, Ke Wang, Jikun Li, Saritha Nellutla, Tatyana I Smirnova, Eric Oldfield.

Abstract

IspG is a 4Fe-4S protein that carries out an essential reduction step in isoprenoid biosynthesis. Using electron-nuclear double resonance (ENDOR) and hyperfine sublevel correlation (HYSCORE) spectroscopies on labeled samples, we have specifically assigned the hyperfine interactions in a reaction intermediate. These results help clarify the nature of the reaction intermediate, supporting a direct interaction between the unique fourth Fe in the cluster and C2 and O3 of the ligand.

Entities: Chemical Disease Gene Species

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Year: 2011 PMID： 21574560 PMCID： PMC3116442 DOI： 10.1021/ja200763a

Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419

23 in total

1. Isoprenoid biosynthesis through the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (GcpE) is a [4Fe-4S] protein.

Authors: Myriam Seemann; Bernadette Tse Sum Bui; Murielle Wolff; Denis Tritsch; Narciso Campos; Albert Boronat; Andrée Marquet; Michel Rohmer
Journal: Angew Chem Int Ed Engl Date: 2002-11-15 Impact factor: 15.336

Review 2. Regulation of the mevalonate pathway.

Authors: J L Goldstein; M S Brown
Journal: Nature Date: 1990-02-01 Impact factor: 49.962

3. LytB protein catalyzes the terminal step of the 2-C-methyl-D-erythritol-4-phosphate pathway of isoprenoid biosynthesis.

Authors: Boran Altincicek; Evert C Duin; Armin Reichenberg; Reiner Hedderich; Ann-Kristin Kollas; Martin Hintz; Stefanie Wagner; Jochen Wiesner; Ewald Beck; Hassan Jomaa
Journal: FEBS Lett Date: 2002-12-18 Impact factor: 4.124

4. Functional characterization of GcpE, an essential enzyme of the non-mevalonate pathway of isoprenoid biosynthesis.

Authors: Ann-Kristin Kollas; Evert C Duin; Matthias Eberl; Boran Altincicek; Martin Hintz; Armin Reichenberg; Dajana Henschker; Anke Henne; Irina Steinbrecher; Dmitry N Ostrovsky; Reiner Hedderich; Ewald Beck; Hassan Jomaa; Jochen Wiesner
Journal: FEBS Lett Date: 2002-12-18 Impact factor: 4.124

5. Possible direct involvement of the active-site [4Fe-4S] cluster of the GcpE enzyme from Thermus thermophilus in the conversion of MEcPP.

Authors: Dolapo Adedeji; Heather Hernandez; Jochen Wiesner; Uwe Köhler; Hassan Jomaa; Evert C Duin
Journal: FEBS Lett Date: 2006-12-19 Impact factor: 4.124

6. Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe-4S] protein.

Authors: Murielle Wolff; Myriam Seemann; Bernadette Tse Sum Bui; Yves Frapart; Denis Tritsch; Ana Garcia Estrabot; Manuel Rodríguez-Concepción; Albert Boronat; Andrée Marquet; Michel Rohmer
Journal: FEBS Lett Date: 2003-04-24 Impact factor: 4.124

7. Structure of the E-1-hydroxy-2-methyl-but-2-enyl-4-diphosphate synthase (GcpE) from Thermus thermophilus.

Authors: Ingo Rekittke; Tsuyoshi Nonaka; Jochen Wiesner; Ulrike Demmer; Eberhard Warkentin; Hassan Jomaa; Ulrich Ermler
Journal: FEBS Lett Date: 2010-12-15 Impact factor: 4.124

8. Deuterium-labelled isotopomers of 2-C-methyl-D-erythritol as tools for the elucidation of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis.

Authors: L Charon; J F Hoeffler; C Pale-Grosdemange; L M Lois; N Campos; A Boronat; M Rohmer
Journal: Biochem J Date: 2000-03-15 Impact factor: 3.857

9. The deoxyxylulose phosphate pathway of isoprenoid biosynthesis: studies on the mechanisms of the reactions catalyzed by IspG and IspH protein.

Authors: Felix Rohdich; Ferdinand Zepeck; Petra Adam; Stefan Hecht; Johannes Kaiser; Ralf Laupitz; Tobias Gräwert; Sabine Amslinger; Wolfgang Eisenreich; Adelbert Bacher; Duilio Arigoni
Journal: Proc Natl Acad Sci U S A Date: 2003-02-05 Impact factor: 11.205

10. A proposed mechanism for the reductive ring opening of the cyclodiphosphate MEcPP, a crucial transformation in the new DXP/MEP pathway to isoprenoids based on modeling studies and feeding experiments.

Authors: Wolfgang Brandt; Marco A Dessoy; Michael Fulhorst; Wenyun Gao; Meinhart H Zenk; Ludger A Wessjohann
Journal: Chembiochem Date: 2004-03-05 Impact factor: 3.164

14 in total

1. A closer look at the spectroscopic properties of possible reaction intermediates in wild-type and mutant (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase.

Authors: Weiya Xu; Nicholas S Lees; Dominique Hall; Dhanushi Welideniya; Brian M Hoffman; Evert C Duin
Journal: Biochemistry Date: 2012-06-07 Impact factor: 3.162

2. Structure, function and inhibition of the two- and three-domain 4Fe-4S IspG proteins.

Authors: Yi-Liang Liu; Francisco Guerra; Ke Wang; Weixue Wang; Jikun Li; Cancan Huang; Wei Zhu; Kevin Houlihan; Zhi Li; Yong Zhang; Satish K Nair; Eric Oldfield
Journal: Proc Natl Acad Sci U S A Date: 2012-05-14 Impact factor: 11.205

Review 3. Advanced paramagnetic resonance spectroscopies of iron-sulfur proteins: Electron nuclear double resonance (ENDOR) and electron spin echo envelope modulation (ESEEM).

Authors: George E Cutsail; Joshua Telser; Brian M Hoffman
Journal: Biochim Biophys Acta Date: 2015-02-14

4. 9-Mercaptodethiobiotin is generated as a ligand to the [2Fe-2S]+ cluster during the reaction catalyzed by biotin synthase from Escherichia coli.

Authors: Corey J Fugate; Troy A Stich; Esther G Kim; William K Myers; R David Britt; Joseph T Jarrett
Journal: J Am Chem Soc Date: 2012-05-29 Impact factor: 15.419

5. Are free radicals involved in IspH catalysis? An EPR and crystallographic investigation.

Authors: Weixue Wang; Ke Wang; Ingrid Span; Johann Jauch; Adelbert Bacher; Michael Groll; Eric Oldfield
Journal: J Am Chem Soc Date: 2012-06-28 Impact factor: 15.419

Review 6. Bioorganometallic chemistry with IspG and IspH: structure, function, and inhibition of the [Fe(4)S(4)] proteins involved in isoprenoid biosynthesis.

Authors: Weixue Wang; Eric Oldfield
Journal: Angew Chem Int Ed Engl Date: 2014-01-31 Impact factor: 15.336

10. Advanced electron paramagnetic resonance on the catalytic iron-sulfur cluster bound to the CCG domain of heterodisulfide reductase and succinate: quinone reductase.

Authors: Alistair J Fielding; Kristian Parey; Ulrich Ermler; Silvan Scheller; Bernhard Jaun; Marina Bennati
Journal: J Biol Inorg Chem Date: 2013-09-14 Impact factor: 3.358