Literature DB >> 4464857

Mammalian chromatin substructure studies with the calcium-magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis.

L A Burgoyne, D R Hewish, J Mobbs.   

Abstract

The basic regularity of chromatin substructure that has been reported in rat liver chromatin (Hewish & Burgoyne, 1973b) was also detected in mouse chromatin. The regular series of DNA fragments produced by the action of Ca-Mg endonuclease on rat chromatin were studied further. The smallest single-stranded class has a molecular weight of approx. 45000-63000 and the smallest double-stranded class has a molecular weight of approx. 120000-150000. Studies of the substructure of the DNA fragments produced by the Ca-Mg endonuclease have shown that the regular series of double-stranded fragments have regular series of single-stranded fragments within them. It was concluded that the regular series of double-stranded fragments was probably a consequence of the regular series of single-stranded fragments. Digestion time-courses are presented for mouse and rat nuclear DNA.

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Year:  1974        PMID： 4464857      PMCID： PMC1168353          DOI： 10.1042/bj1430067

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  23 in total

1.  Electrophoretic properties of low molecular weight DNA fragments in agarose-acrylamide gels.

Authors:  C W Dingman; T Kakefuda; M P Fisher
Journal:  Anal Biochem       Date:  1972-12       Impact factor: 3.365

2.  The calcium dependent endonuclease activity of isolated nuclear preparations. Relationships between its occurrence and the occurrence of other classes of enzymes found in nuclear preparations.

Authors:  D R Hewish; L A Burgoyne
Journal:  Biochem Biophys Res Commun       Date:  1973-05-15       Impact factor: 3.575

3.  The identification of different structural classes of nucleic acids by electrophoresis in polyacrylamide gels of different concentration.

Authors:  E H Harley; J S White; K R Rees
Journal:  Biochim Biophys Acta       Date:  1973-03-19

4.  Effect of poly(ADP-ribose) formation on DNA synthesis and DNA fragmentation in nuclei of rat liver and rat ascites hepatoma AH-130 cells.

Authors:  M Yamada; M Nagao; T Hidaka; T Sugimura
Journal:  Biochem Biophys Res Commun       Date:  1973-10-15       Impact factor: 3.575

5.  Role of molecular conformation in determining the electrophoretic properties of polynucleotides in agarose-acrylamide gels. II.

Authors:  C W Dingman; M P Fisher; T Kakefuda
Journal:  Biochemistry       Date:  1972-03-28       Impact factor: 3.162

6.  Blocking by histones of accessibility to DNA in chromatin.

Authors:  A E Mirsky; B Silverman
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

7.  Blocking by histones of accessibility to DNA in chromatin: addition of histones.

Authors:  A E Mirsky; B Silverman; N C Panda
Journal:  Proc Natl Acad Sci U S A       Date:  1972-11       Impact factor: 11.205

8.  Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease.

Authors:  D R Hewish; L A Burgoyne
Journal:  Biochem Biophys Res Commun       Date:  1973-05-15       Impact factor: 3.575

9.  The structure of chromatin as revealed by deoxyribonuclease digestion studies.

Authors:  R J Billing; J Bonner
Journal:  Biochim Biophys Acta       Date:  1972-10-27

10.  Addition of histones to histone-depleted nuclei: effect on template activity toward DNA and RNA polymerases.

Authors:  B Silverman; A E Mirsky
Journal:  Proc Natl Acad Sci U S A       Date:  1973-09       Impact factor: 11.205
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  37 in total

1.  Evidence for a subunit structure of chromatin in mouse myeloma cells.

Authors:  J D McGhee; C B Kimmel
Journal:  Chromosoma       Date:  1975-09-26       Impact factor: 4.316

2.  The reaction of the Ca-Mg endonuclease with the A-sites of rat nucleoprotein.

Authors:  L A Burgoyne; J Mobbs
Journal:  Nucleic Acids Res       Date:  1975-09       Impact factor: 16.971

3.  An approach to histone nearest neighbours in extended chromatin.

Authors:  R C Hardison; M E Eichner; R Chalkley
Journal:  Nucleic Acids Res       Date:  1975-10       Impact factor: 16.971

4.  Chromatin structure: a property of the higher structures of chromatin and in the time course of its formation during chromatin replication.

Authors:  L A Burgoyne; J D Mobbs; A J Marshall
Journal:  Nucleic Acids Res       Date:  1976-12       Impact factor: 16.971

5.  Chromatin nu bodies: isolation, subfractionation and physical characterization.

Authors:  A L Olins; R D Carlson; E B Wright; D E Olins
Journal:  Nucleic Acids Res       Date:  1976-12       Impact factor: 16.971

6.  Photochemical addition of the cross-linking reagent 4,5', 8-trimethylpsoralen (trioxaslen) to intracellular and viral simian virus 40 DNA-histone complexes.

Authors:  L M Hallick; H A Yokota; J C Bartholomew; J E Hearst
Journal:  J Virol       Date:  1978-07       Impact factor: 5.103

7.  The same amount of DNA is organized in in vitro-assembled nucleosomes irrespective of the origin of the histones.

Authors:  C Spadafora; P Oudet; P Chambon
Journal:  Nucleic Acids Res       Date:  1978-10       Impact factor: 16.971

8.  Aspirin-induced mucosal cell death in human gastric cells: evidence supporting an apoptotic mechanism.

Authors:  Jacinda J Power; Miranda S Dennis; Maria J Redlak; Thomas A Miller
Journal:  Dig Dis Sci       Date:  2004-09       Impact factor: 3.199

9.  Structural defects in rat liver deoxyribonucleic acid. Endogenous single-strained regions in comparison with damage induced in vivo by a carcinogen.

Authors:  B W Stewart; P H Huang; M J Brian
Journal:  Biochem J       Date:  1979-05-01       Impact factor: 3.857

10.  Ligation and synthesis of chromatin deoxyribonucleic acid in vitro in neuronal, glial and liver nuclei isolated from adult guinea pig.

Authors:  N Inoue; T Ono; T Kato
Journal:  Biochem J       Date:  1979-06-15       Impact factor: 3.857
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