Literature DB >> 2138783

A method for difference cloning: gene amplification following subtractive hybridization.

I Wieland1, G Bolger, G Asouline, M Wigler.   

Abstract

We describe a procedure for genomic difference cloning, a method for isolating sequences present in one genomic DNA population ("tester") that is absent in another ("driver"). By subtractive hybridization, a large excess of driver is used to remove sequences common to a biotinylated tester, enriching the "target" sequences that are unique to the tester. After repeated subtractive hybridization cycles, tester is separated from driver by avidin/biotin affinity chromatography, and single-stranded target is amplified by the polymerase chain reaction, rendering it double-stranded and clonable. We model two situations: the gain of sequences that result from infection with a pathogen and the loss of sequences that result from a large hemizygous deletion. We obtain 100- to 700-fold enrichment of target sequences.

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Year:  1990        PMID： 2138783      PMCID： PMC53762          DOI： 10.1073/pnas.87.7.2720

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  23 in total

1.  Characterization of an activated human ros gene.

Authors:  C Birchmeier; D Birnbaum; G Waitches; O Fasano; M Wigler
Journal:  Mol Cell Biol       Date:  1986-09       Impact factor: 4.272

2.  Affinity chromatography of DNA labeled with chemically cleavable biotinylated nucleotide analogs.

Authors:  T M Herman; E Lefever; M Shimkus
Journal:  Anal Biochem       Date:  1986-07       Impact factor: 3.365

3.  Production of single-stranded plasmid DNA.

Authors:  J Vieira; J Messing
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

Review 4.  Molecular genetics: applications to the clinical neurosciences.

Authors:  J B Martin
Journal:  Science       Date:  1987-11-06       Impact factor: 47.728

5.  New human transforming genes detected by a tumorigenicity assay.

Authors:  O Fasano; D Birnbaum; L Edlund; J Fogh; M Wigler
Journal:  Mol Cell Biol       Date:  1984-09       Impact factor: 4.272

6.  SV40 large tumor antigen can regulate some cellular transcripts in a positive fashion.

Authors:  T Schutzbank; R Robinson; M Oren; A J Levine
Journal:  Cell       Date:  1982-09       Impact factor: 41.582

7.  Activation of mouse genes in transformed cells.

Authors:  M R Scott; K H Westphal; P W Rigby
Journal:  Cell       Date:  1983-09       Impact factor: 41.582

8.  Isolation of anonymous DNA sequences from within a submicroscopic X chromosomal deletion in a patient with choroideremia, deafness, and mental retardation.

Authors:  R L Nussbaum; J G Lesko; R A Lewis; S A Ledbetter; D H Ledbetter
Journal:  Proc Natl Acad Sci U S A       Date:  1987-09       Impact factor: 11.205

9.  Y-encoded, species-specific DNA in mice: evidence that the Y chromosome exists in two polymorphic forms in inbred strains.

Authors:  E E Lamar; E Palmer
Journal:  Cell       Date:  1984-05       Impact factor: 41.582

10.  Specific cloning of DNA fragments absent from the DNA of a male patient with an X chromosome deletion.

Authors:  L M Kunkel; A P Monaco; W Middlesworth; H D Ochs; S A Latt
Journal:  Proc Natl Acad Sci U S A       Date:  1985-07       Impact factor: 11.205
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  24 in total

1.  Removal of known, abundant cDNA species by specific double-stranded cDNA synthesis-based subtraction.

Authors:  X O Yang; X D Huang; H L Zhao; J W Zhang
Journal:  Mol Biotechnol       Date:  1999-06       Impact factor: 2.695

2.  Construction of spinal cord cDNA library and application for subtractive cloning of spinal cord-specific cDNAs.

Authors:  H Kobayashi; H Takahashi; K Oyanagi; F Ikuta; T Miyatake; S Tsuji
Journal:  J Mol Neurosci       Date:  1991       Impact factor: 3.444

3.  A gene expression screen.

Authors:  Z Wang; D D Brown
Journal:  Proc Natl Acad Sci U S A       Date:  1991-12-15       Impact factor: 11.205

4.  Sequenase should be used instead of the Klenow fragment for the synthesis of oligonucleotides labeled to a high specific activity.

Authors:  G Rigaud; T Grange; R Pictet
Journal:  Nucleic Acids Res       Date:  1991-09-11       Impact factor: 16.971

5.  PCR driven DNA-DNA competitive hybridization: a new method for sensitive differential cloning.

Authors:  M C Lebeau; G Alvarez-Bolado; W Wahli; S Catsicas
Journal:  Nucleic Acids Res       Date:  1991-09-11       Impact factor: 16.971

6.  Discovery of estrogen-responsive genes using an improved method which combines subtractive hybridization and PCR.

Authors:  W Liu; W Su; T M Roberts
Journal:  Nucleic Acids Res       Date:  1998-08-01       Impact factor: 16.971

7.  A simulation of subtractive hybridization.

Authors:  T J Cho; S S Park
Journal:  Nucleic Acids Res       Date:  1998-03-15       Impact factor: 16.971

8.  Identification by representational difference analysis of a homozygous deletion in pancreatic carcinoma that lies within the BRCA2 region.

Authors:  M Schutte; L T da Costa; S A Hahn; C Moskaluk; A T Hoque; E Rozenblum; C L Weinstein; M Bittner; P S Meltzer; J M Trent
Journal:  Proc Natl Acad Sci U S A       Date:  1995-06-20       Impact factor: 11.205

9.  Positive selection of candidate tumor-suppressor genes by subtractive hybridization.

Authors:  S W Lee; C Tomasetto; R Sager
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-01       Impact factor: 11.205

10.  Isolation and characterization of allelic losses and gains in colorectal tumors by arbitrarily primed polymerase chain reaction.

Authors:  M A Peinado; S Malkhosyan; A Velazquez; M Perucho
Journal:  Proc Natl Acad Sci U S A       Date:  1992-11-01       Impact factor: 11.205
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