Literature DB >> 1722336

A gene expression screen.

Z Wang1, D D Brown.   

Abstract

A gene expression screen identifies mRNAs that differ in abundance between two mRNA mixtures by a subtractive hybridization method. The two mRNA populations are converted to double-stranded cDNAs, fragmented, and ligated to linkers for polymerase chain reaction (PCR) amplification. The multiple cDNA fragments isolated from any given gene can be treated as alleles in a genetic screen. Probability analysis of the frequency with which multiple alleles are found provides an estimation of the total number of up- and down-regulated genes. We have applied this method to genes that are differentially expressed in amphibian tadpole tail tissue in the first 24 hr after thyroid hormone treatment, which ultimately induces tail resorption. We estimate that there are about 30 up-regulated genes; 16 have been isolated.

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Year:  1991        PMID： 1722336      PMCID： PMC53164          DOI： 10.1073/pnas.88.24.11505

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  18 in total

1.  A correlation of thyroid hormone receptor gene expression with amphibian metamorphosis.

Authors:  Y Yaoita; D D Brown
Journal:  Genes Dev       Date:  1990-11       Impact factor: 11.361

2.  Low-ratio hybridization subtraction.

Authors:  J Fargnoli; N J Holbrook; A J Fornace
Journal:  Anal Biochem       Date:  1990-06       Impact factor: 3.365

3.  Selective enrichment of specific DNA, cDNA and RNA sequences using biotinylated probes, avidin and copper-chelate agarose.

Authors:  A A Welcher; A R Torres; D C Ward
Journal:  Nucleic Acids Res       Date:  1986-12-22       Impact factor: 16.971

4.  Subtractive hybridization system using single-stranded phagemids with directional inserts.

Authors:  J L Rubenstein; A E Brice; R D Ciaranello; D Denney; M H Porteus; T B Usdin
Journal:  Nucleic Acids Res       Date:  1990-08-25       Impact factor: 16.971

5.  A simple subtractive hybridization technique employing photoactivatable biotin and phenol extraction.

Authors:  H L Sive; T St John
Journal:  Nucleic Acids Res       Date:  1988-11-25       Impact factor: 16.971

6.  Expression of a single transfected cDNA converts fibroblasts to myoblasts.

Authors:  R L Davis; H Weintraub; A B Lassar
Journal:  Cell       Date:  1987-12-24       Impact factor: 41.582

7.  Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells.

Authors:  F W Alt; R E Kellems; J R Bertino; R T Schimke
Journal:  J Biol Chem       Date:  1978-03-10       Impact factor: 5.157

8.  Cell type-specific activation of actin genes in the early amphibian embryo.

Authors:  T J Mohun; S Brennan; N Dathan; S Fairman; J B Gurdon
Journal:  Nature       Date:  1984 Oct 25-31       Impact factor: 49.962

9.  Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridization of a cDNA library.

Authors:  J R Duguid; R G Rohwer; B Seed
Journal:  Proc Natl Acad Sci U S A       Date:  1988-08       Impact factor: 11.205

10.  Identification of a set of genes expressed during the G0/G1 transition of cultured mouse cells.

Authors:  L F Lau; D Nathans
Journal:  EMBO J       Date:  1985-12-01       Impact factor: 11.598
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  69 in total

1.  Identification of the immediate-early transcripts of Kaposi's sarcoma-associated herpesvirus.

Authors:  F X Zhu; T Cusano; Y Yuan
Journal:  J Virol       Date:  1999-07       Impact factor: 5.103

2.  Isolation of rapeseed genes expressed early and specifically during development of the male gametophyte.

Authors:  A Fourgoux-Nicol; J Drouaud; N Haouazine; G Pelletier; P Guerche
Journal:  Plant Mol Biol       Date:  1999-07       Impact factor: 4.076

3.  Identification of genes specifically expressed in cauliflower reproductive meristems. Molecular characterization of BoREM1.

Authors:  J M Franco-Zorrilla; B Fernández-Calvín; F Madueño; M Cruz-Alvarez; J Salinas; J M Martínez-Zapater
Journal:  Plant Mol Biol       Date:  1999-02       Impact factor: 4.076

4.  A critical evaluation of differential display as a tool to identify genes involved in legume nodulation: looking back and looking forward.

Authors:  S Lievens; S Goormachtig; M Holsters
Journal:  Nucleic Acids Res       Date:  2001-09-01       Impact factor: 16.971

5.  Diverse developmental programs of Xenopus laevis metamorphosis are inhibited by a dominant negative thyroid hormone receptor.

Authors:  A M Schreiber; B Das; H Huang; N Marsh-Armstrong; D D Brown
Journal:  Proc Natl Acad Sci U S A       Date:  2001-08-21       Impact factor: 11.205

6.  Identification of differentially expressed genes in isogenic highly metastatic and poorly metastatic cell lines of R3230AC rat mammary adenocarcinoma.

Authors:  H Günes; S A Carlsen
Journal:  Cell Prolif       Date:  2003-12       Impact factor: 6.831

7.  Gene expression in cadmium-tolerant Datura innoxia: detection and characterization of cDNAs induced in response to Cd2+.

Authors:  Maggie Louie; Nathan Kondor; Jane G DeWitt
Journal:  Plant Mol Biol       Date:  2003-05       Impact factor: 4.076

8.  Inhibition of 5alpha-reductase in rat prostate reveals differential regulation of androgen-response gene expression by testosterone and dihydrotestosterone.

Authors:  S S Dadras; X Cai; I Abasolo; Z Wang
Journal:  Gene Expr       Date:  2001

9.  Astrocytes phagocytose focal dystrophies from shortening myelin segments in the optic nerve of Xenopus laevis at metamorphosis.

Authors:  Elizabeth A Mills; Chung-ha O Davis; Eric A Bushong; Daniela Boassa; Keun-Young Kim; Mark H Ellisman; Nicholas Marsh-Armstrong
Journal:  Proc Natl Acad Sci U S A       Date:  2015-08-03       Impact factor: 11.205

10.  AML1 is expressed in skeletal muscle and is regulated by innervation.

Authors:  X Zhu; J E Yeadon; S J Burden
Journal:  Mol Cell Biol       Date:  1994-12       Impact factor: 4.272
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