Literature DB >> 10503239

Removal of known, abundant cDNA species by specific double-stranded cDNA synthesis-based subtraction.

X O Yang1, X D Huang, H L Zhao, J W Zhang.   

Abstract

An efficient and simple method for removing known, abundant cDNA species from the cDNA pool of highly differentiated cells is reported. The method involves preparation of sscDNA, followed by dscDNA-synthesis of known, abundant cDNA species led by specific primers and removal of the synthesized dscDNA with hydroxyapatite. By using this method, the globin cDNAs were reduced to less than 10(-5) of their original abundance. The results suggest that this method may facilitate the isolation of new genes from specific cells or tissues.

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Year:  1999        PMID： 10503239     DOI： 10.1007/BF02788681

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  6 in total

1.  A method for difference cloning: gene amplification following subtractive hybridization.

Authors:  I Wieland; G Bolger; G Asouline; M Wigler
Journal:  Proc Natl Acad Sci U S A       Date:  1990-04       Impact factor: 11.205

2.  Construction of quantitative RT-PCR MIMICs.

Authors:  F Borriello; J Lederer
Journal:  Biotechniques       Date:  1995-10       Impact factor: 1.993

3.  Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.

Authors:  U B Gyllensten; H A Erlich
Journal:  Proc Natl Acad Sci U S A       Date:  1988-10       Impact factor: 11.205

4.  WT1 as a new prognostic factor and a new marker for the detection of minimal residual disease in acute leukemia.

Authors:  K Inoue; H Sugiyama; H Ogawa; M Nakagawa; T Yamagami; H Miwa; K Kita; A Hiraoka; T Masaoka; K Nasu
Journal:  Blood       Date:  1994-11-01       Impact factor: 22.113

5.  Positive selection of candidate tumor-suppressor genes by subtractive hybridization.

Authors:  S W Lee; C Tomasetto; R Sager
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-01       Impact factor: 11.205

6.  Coexpression of gamma and beta globin mRNA in cells containing a single human beta globin locus: results from studies using single-cell reverse transcription polymerase chain reaction.

Authors:  T Furukawa; G Zitnik; K Leppig; T Papayannopoulou; G Stamatoyannopoulos
Journal:  Blood       Date:  1994-03-01       Impact factor: 22.113
  6 in total

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