Literature DB >> 6092933

New human transforming genes detected by a tumorigenicity assay.

O Fasano, D Birnbaum, L Edlund, J Fogh, M Wigler.   

Abstract

We have developed a sensitive bioassay for transforming genes based on the tumorigenicity of cotransfected NIH3T3 cells in nude mice. The assay differs substantially from the NIH3T3 focus assay. Using it, we have detected the transfer of three transforming genes from the DNA of MCF-7, a human mammary carcinoma cell line. One of these is N-ras, which is amplified in MCF-7 DNA. The other two, which we have called mcf2 and mcf3, do not appear to be related to known oncogenes. We cannot detect their transfer by using the NIH3T3 focus assay. We do not yet know whether either mcf2 or mcf3 is associated with genetic abnormalities in MCF-7 cells.

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Year:  1984        PMID： 6092933      PMCID： PMC368975          DOI： 10.1128/mcb.4.9.1695-1705.1984

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  54 in total

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Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

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Journal:  Cell       Date:  1978-05       Impact factor: 41.582

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Authors:  B Hohn; K Murray
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Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

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Journal:  J Natl Cancer Inst       Date:  1973-11       Impact factor: 13.506

6.  A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors:  F L Graham; A J van der Eb
Journal:  Virology       Date:  1973-04       Impact factor: 3.616

7.  Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid.

Authors:  T Tanaka; B Weisblum
Journal:  J Bacteriol       Date:  1975-01       Impact factor: 3.490

8.  Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.

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Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

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Journal:  J Virol       Date:  1969-11       Impact factor: 5.103

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Authors:  T Maniatis; A Jeffrey; D G Kleid
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205
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  76 in total

1.  Loss of the amino-terminal helix-loop-helix domain of the vav proto-oncogene activates its transforming potential.

Authors:  S Katzav; J L Cleveland; H E Heslop; D Pulido
Journal:  Mol Cell Biol       Date:  1991-04       Impact factor: 4.272

2.  Expression and rearrangement of the ROS1 gene in human glioblastoma cells.

Authors:  C Birchmeier; S Sharma; M Wigler
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

3.  Transforming genes and chromosome aberrations in therapy-related leukemia and myelodysplastic syndrome.

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Journal:  Ann Hematol       Date:  1991-06       Impact factor: 3.673

4.  Differentiation, not determination, regulates muscle gene activation: transfection of troponin I genes into multipotential and muscle lineages of 10T1/2 cells.

Authors:  S F Konieczny; C P Emerson
Journal:  Mol Cell Biol       Date:  1985-09       Impact factor: 4.272

5.  Characterization of c-Ki-ras and N-ras oncogenes in aflatoxin B1-induced rat liver tumors.

Authors:  G McMahon; E F Davis; L J Huber; Y Kim; G N Wogan
Journal:  Proc Natl Acad Sci U S A       Date:  1990-02       Impact factor: 11.205

6.  A method for difference cloning: gene amplification following subtractive hybridization.

Authors:  I Wieland; G Bolger; G Asouline; M Wigler
Journal:  Proc Natl Acad Sci U S A       Date:  1990-04       Impact factor: 11.205

7.  Development and analysis of a transformation-defective mutant of Harvey murine sarcoma tk virus and its gene product.

Authors:  M O Weeks; G L Hager; R Lowe; E M Scolnick
Journal:  J Virol       Date:  1985-05       Impact factor: 5.103

8.  An in vivo screening system to identify tumorigenic genes.

Authors:  T Ihara; Y Hosokawa; K Kumazawa; K Ishikawa; J Fujimoto; M Yamamoto; T Muramkami; N Goshima; E Ito; S Watanabe; K Semba
Journal:  Oncogene       Date:  2016-10-03       Impact factor: 9.867

9.  Identification of the human prostatic carcinoma oncogene PTI-1 by rapid expression cloning and differential RNA display.

Authors:  R Shen; Z Z Su; C A Olsson; P B Fisher
Journal:  Proc Natl Acad Sci U S A       Date:  1995-07-18       Impact factor: 11.205

10.  Expression of transfected mutant beta-actin genes: transitions toward the stable tumorigenic state.

Authors:  J Leavitt; S Y Ng; M Varma; G Latter; S Burbeck; P Gunning; L Kedes
Journal:  Mol Cell Biol       Date:  1987-07       Impact factor: 4.272
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