Literature DB >> 21297618

High-throughput single-molecule optofluidic analysis.

Soohong Kim1, Aaron M Streets, Ron R Lin, Stephen R Quake, Shimon Weiss, Devdoot S Majumdar.   

Abstract

We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.

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Year:  2011        PMID： 21297618      PMCID： PMC3075913          DOI： 10.1038/nmeth.1569

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  21 in total

1.  Monolithic microfabricated valves and pumps by multilayer soft lithography.

Authors:  M A Unger; H P Chou; T Thorsen; A Scherer; S R Quake
Journal:  Science       Date:  2000-04-07       Impact factor: 47.728

2.  Modeling of DNA hybridization kinetics for spatially resolved biochips.

Authors:  David Erickson; Dongqing Li; Ulrich J Krull
Journal:  Anal Biochem       Date:  2003-06-15       Impact factor: 3.365

3.  Fluorescence-aided molecule sorting: analysis of structure and interactions by alternating-laser excitation of single molecules.

Authors:  Achillefs N Kapanidis; Nam Ki Lee; Ted A Laurence; Sören Doose; Emmanuel Margeat; Shimon Weiss
Journal:  Proc Natl Acad Sci U S A       Date:  2004-06-02       Impact factor: 11.205

4.  Protein folding: defining a "standard" set of experimental conditions and a preliminary kinetic data set of two-state proteins.

Authors:  Karen L Maxwell; David Wildes; Arash Zarrine-Afsar; Miguel A De Los Rios; Andrew G Brown; Claire T Friel; Linda Hedberg; Jia-Cherng Horng; Diane Bona; Erik J Miller; Alexis Vallée-Bélisle; Ewan R G Main; Francesco Bemporad; Linlin Qiu; Kaare Teilum; Ngoc-Diep Vu; Aled M Edwards; Ingo Ruczinski; Flemming M Poulsen; Birthe B Kragelund; Stephen W Michnick; Fabrizio Chiti; Yawen Bai; Stephen J Hagen; Luis Serrano; Mikael Oliveberg; Daniel P Raleigh; Pernilla Wittung-Stafshede; Sheena E Radford; Sophie E Jackson; Tobin R Sosnick; Susan Marqusee; Alan R Davidson; Kevin W Plaxco
Journal:  Protein Sci       Date:  2005-02-02       Impact factor: 6.725

5.  Femtomole mixer for microsecond kinetic studies of protein folding.

Authors:  David E Hertzog; Xavier Michalet; Marcus Jäger; Xiangxu Kong; Juan G Santiago; Shimon Weiss; Olgica Bakajin
Journal:  Anal Chem       Date:  2004-12-15       Impact factor: 6.986

6.  A microfluidic mixing system for single-molecule measurements.

Authors:  Shawn H Pfeil; Charles E Wickersham; Armin Hoffmann; Everett A Lipman
Journal:  Rev Sci Instrum       Date:  2009-05       Impact factor: 1.523

7.  Single-molecule measurement of protein folding kinetics.

Authors:  Everett A Lipman; Benjamin Schuler; Olgica Bakajin; William A Eaton
Journal:  Science       Date:  2003-08-29       Impact factor: 47.728

8.  Thermodynamic parameters to predict stability of RNA/DNA hybrid duplexes.

Authors:  N Sugimoto; S Nakano; M Katoh; A Matsumura; H Nakamuta; T Ohmichi; M Yoneyama; M Sasaki
Journal:  Biochemistry       Date:  1995-09-05       Impact factor: 3.162

9.  Nonequilibrium single molecule protein folding in a coaxial mixer.

Authors:  Kambiz M Hamadani; Shimon Weiss
Journal:  Biophys J       Date:  2008-03-13       Impact factor: 4.033

10.  High-resolution temperature-concentration diagram of alpha-synuclein conformation obtained from a single Förster resonance energy transfer image in a microfluidic device.

Authors:  Virginia Vandelinder; Allan Chris M Ferreon; Yann Gambin; Ashok A Deniz; Alex Groisman
Journal:  Anal Chem       Date:  2009-08-15       Impact factor: 6.986
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  33 in total

Review 1.  Single-molecule views of protein movement on single-stranded DNA.

Authors:  Taekjip Ha; Alexander G Kozlov; Timothy M Lohman
Journal:  Annu Rev Biophys       Date:  2012-02-23       Impact factor: 12.981

2.  Linear conversion of pressure into concentration, rapid switching of concentration, and generation of linear ramps of concentration in a microfluidic device.

Authors:  Micha Adler; Alex Groisman
Journal:  Biomicrofluidics       Date:  2012-04-13       Impact factor: 2.800

3.  A triple threat to single molecules.

Authors:  Martin Gruebele
Journal:  Nat Methods       Date:  2011-03       Impact factor: 28.547

4.  An in vitro tag-and-modify protein sample generation method for single-molecule fluorescence resonance energy transfer.

Authors:  Kambiz M Hamadani; Jesse Howe; Madeleine K Jensen; Peng Wu; Jamie H D Cate; Susan Marqusee
Journal:  J Biol Chem       Date:  2017-07-28       Impact factor: 5.157

5.  Different types of pausing modes during transcription initiation.

Authors:  Eitan Lerner; Antonino Ingargiola; Jookyung J Lee; Sergei Borukhov; Xavier Michalet; Shimon Weiss
Journal:  Transcription       Date:  2017-03-23

6.  Chip in a lab: Microfluidics for next generation life science research.

Authors:  Aaron M Streets; Yanyi Huang
Journal:  Biomicrofluidics       Date:  2013-01-31       Impact factor: 2.800

7.  Four-color alternating-laser excitation single-molecule fluorescence spectroscopy for next-generation biodetection assays.

Authors:  Seok W Yim; Taiho Kim; Ted A Laurence; Steve Partono; Dongsik Kim; Younggyu Kim; Shimon Weiss; Armin Reitmair
Journal:  Clin Chem       Date:  2012-01-19       Impact factor: 8.327

8.  Single-molecule methods leap ahead.

Authors:  Taekjip Ha
Journal:  Nat Methods       Date:  2014-10       Impact factor: 28.547

9.  Characterizing highly dynamic conformational states: The transcription bubble in RNAP-promoter open complex as an example.

Authors:  Eitan Lerner; Antonino Ingargiola; Shimon Weiss
Journal:  J Chem Phys       Date:  2018-03-28       Impact factor: 3.488

10.  Lab-on-a-chip technologies for single-molecule studies.

Authors:  Yanhui Zhao; Danqi Chen; Hongjun Yue; Jarrod B French; Joseph Rufo; Stephen J Benkovic; Tony Jun Huang
Journal:  Lab Chip       Date:  2013-05-14       Impact factor: 6.799
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