Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Femtomole mixer for microsecond kinetic studies of protein folding.

Literature DB >> 15595857

Femtomole mixer for microsecond kinetic studies of protein folding.

David E Hertzog¹, Xavier Michalet, Marcus Jäger, Xiangxu Kong, Juan G Santiago, Shimon Weiss, Olgica Bakajin.

Abstract

We have developed a microfluidic mixer for studying protein folding and other reactions with a mixing time of 8 mus and sample consumption of femtomoles. This device enables us to access conformational changes under conditions far from equilibrium and at previously inaccessible time scales. In this paper, we discuss the design and optimization of the mixer using modeling of convective diffusion phenomena and a characterization of the mixer performance using microparticle image velocimetry, dye quenching, and Forster resonance energy-transfer (FRET) measurements of single-stranded DNA. We also demonstrate the feasibility of measuring fast protein folding kinetics using FRET with acyl-CoA binding protein.

Mesh：

Substances：

Year: 2004 PMID： 15595857 PMCID： PMC1413504 DOI： 10.1021/ac048661s

Source DB: PubMed Journal: Anal Chem ISSN： 0003-2700 Impact factor: 6.986

32 in total

1. Quantitative analysis of molecular interaction in a microfluidic channel: the T-sensor.

Authors: A E Kamholz; B H Weigl; B A Finlayson; P Yager
Journal: Anal Chem Date: 1999-12-01 Impact factor: 6.986

2. Compactness of the denatured state of a fast-folding protein measured by submillisecond small-angle x-ray scattering.

Authors: L Pollack; M W Tate; N C Darnton; J B Knight; S M Gruner; W A Eaton; R H Austin
Journal: Proc Natl Acad Sci U S A Date: 1999-08-31 Impact factor: 11.205

3. Ultrahigh-resolution multicolor colocalization of single fluorescent probes.

Authors: T D Lacoste; X Michalet; F Pinaud; D S Chemla; A P Alivisatos; S Weiss
Journal: Proc Natl Acad Sci U S A Date: 2000-08-15 Impact factor: 11.205

Review 4. On-chip enzymatic assays.

Authors: Joseph Wang
Journal: Electrophoresis Date: 2002-03 Impact factor: 3.535

5. Time resolved collapse of a folding protein observed with small angle x-ray scattering.

Authors: L Pollack; M W Tate; A C Finnefrock; C Kalidas; S Trotter; N C Darnton; L Lurio; R H Austin; C A Batt; S M Gruner; S G Mochrie
Journal: Phys Rev Lett Date: 2001-05-21 Impact factor: 9.161

6. Kinetics and mechanism of the folding of cytochrome c.

Authors: K M Pryse; T G Bruckman; B W Maxfield; E L Elson
Journal: Biochemistry Date: 1992-06-09 Impact factor: 3.162

7. Biotechnology at low Reynolds numbers.

Authors: J P Brody; P Yager; R E Goldstein; R H Austin
Journal: Biophys J Date: 1996-12 Impact factor: 4.033

8. Submillisecond protein folding kinetics studied by ultrarapid mixing.

Authors: C K Chan; Y Hu; S Takahashi; D L Rousseau; W A Eaton; J Hofrichter
Journal: Proc Natl Acad Sci U S A Date: 1997-03-04 Impact factor: 11.205

9. Stepwise formation of alpha-helices during cytochrome c folding.

Authors: S Akiyama; S Takahashi; K Ishimori; I Morishima
Journal: Nat Struct Biol Date: 2000-06

10. Reduction of fibrous adhesion formation by a copolymer possessing an affinity for anionic surfaces.

Authors: D L Elbert; J A Hubbell
Journal: J Biomed Mater Res Date: 1998-10

34 in total

1. Visualizing millisecond chaotic mixing dynamics in microdroplets: A direct comparison of experiment and simulation.

Authors: Liguo Jiang; Yan Zeng; Hongbo Zhou; Jianan Y Qu; Shuhuai Yao
Journal: Biomicrofluidics Date: 2012-03-15 Impact factor: 2.800

2. Protein denaturation and protein:drugs interactions from intrinsic protein fluorescence measurements at the nanolitre scale.

Authors: Matthieu Gaudet; Nina Remtulla; Sophie E Jackson; Ewan R G Main; Daniel G Bracewell; Gabriel Aeppli; Paul A Dalby
Journal: Protein Sci Date: 2010-08 Impact factor: 6.725

3. Molecular fluorescence, phosphorescence, and chemiluminescence spectrometry.

Authors: Kristin A Fletcher; Sayo O Fakayode; Mark Lowry; Sheryl A Tucker; Sharon L Neal; Irene W Kimaru; Matthew E McCarroll; Gabor Patonay; Philip B Oldham; Oleksandr Rusin; Robert M Strongin; Isiah M Warner
Journal: Anal Chem Date: 2006-06-15 Impact factor: 6.986

Femtomole mixer for microsecond kinetic studies of protein folding.

1. Quantitative analysis of molecular interaction in a microfluidic channel: the T-sensor.

2. Compactness of the denatured state of a fast-folding protein measured by submillisecond small-angle x-ray scattering.

3. Ultrahigh-resolution multicolor colocalization of single fluorescent probes.

Review 4. On-chip enzymatic assays.

5. Time resolved collapse of a folding protein observed with small angle x-ray scattering.

6. Kinetics and mechanism of the folding of cytochrome c.

7. Biotechnology at low Reynolds numbers.

8. Submillisecond protein folding kinetics studied by ultrarapid mixing.

9. Stepwise formation of alpha-helices during cytochrome c folding.

10. Reduction of fibrous adhesion formation by a copolymer possessing an affinity for anionic surfaces.

1. Visualizing millisecond chaotic mixing dynamics in microdroplets: A direct comparison of experiment and simulation.

2. Protein denaturation and protein:drugs interactions from intrinsic protein fluorescence measurements at the nanolitre scale.

3. Molecular fluorescence, phosphorescence, and chemiluminescence spectrometry.

Review 4. Single-molecule fluorescence studies of protein folding and conformational dynamics.

5. Dynamics of synaptic SfiI-DNA complex: single-molecule fluorescence analysis.

6. Measuring the folding transition time of single RNA molecules.

7. Protein hydrophobic collapse and early folding steps observed in a microfluidic mixer.

8. Ruggedness in the folding landscape of protein L.

Review 9. Protein folding transition path times from single molecule FRET.

Review 10. Invited review article: Imaging techniques for harmonic and multiphoton absorption fluorescence microscopy.