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Literature DB >> 21278722

Five challenges to bringing single-molecule force spectroscopy into living cells.

Yves F Dufrêne¹, Evan Evans, Andreas Engel, Jonne Helenius, Hermann E Gaub, Daniel J Müller.

Abstract

In recent years, single-molecule force spectroscopy techniques have been used to study how inter- and intramolecular interactions control the assembly and functional state of biomolecular machinery in vitro. Here we discuss the problems and challenges that need to be addressed to bring these technologies into living cells and to learn how cellular machinery is controlled in vivo.

Mesh：

Year: 2011 PMID： 21278722 DOI： 10.1038/nmeth0211-123

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

25 in total

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Authors: Christoph Gerber; Hans Peter Lang
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Authors: Carol V Robinson; Andrej Sali; Wolfgang Baumeister
Journal: Nature Date: 2007-12-13 Impact factor: 49.962

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Authors: Keir C Neuman; Attila Nagy
Journal: Nat Methods Date: 2008-06 Impact factor: 28.547

Review 7. Structure and mechanics of membrane proteins.

Authors: Andreas Engel; Hermann E Gaub
Journal: Annu Rev Biochem Date: 2008 Impact factor: 23.643

Review 8. A practical guide to single-molecule FRET.

Authors: Rahul Roy; Sungchul Hohng; Taekjip Ha
Journal: Nat Methods Date: 2008-06 Impact factor: 28.547

9. Consequences of motor copy number on the intracellular transport of kinesin-1-driven lipid droplets.

Authors: George T Shubeita; Susan L Tran; Jing Xu; Michael Vershinin; Silvia Cermelli; Sean L Cotton; Michael A Welte; Steven P Gross
Journal: Cell Date: 2008-12-12 Impact factor: 41.582

10. Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics.

Authors: Carsten Grashoff; Brenton D Hoffman; Michael D Brenner; Ruobo Zhou; Maddy Parsons; Michael T Yang; Mark A McLean; Stephen G Sligar; Christopher S Chen; Taekjip Ha; Martin A Schwartz
Journal: Nature Date: 2010-07-08 Impact factor: 49.962

39 in total

1. Analyzing the micromechanics of the cell division apparatus.

Authors: Yuta Shimamoto; Tarun M Kapoor
Journal: Methods Cell Biol Date: 2018-05-01 Impact factor: 1.441

2. Characterization of the mechanical properties of cancer cells in 3D matrices in response to collagen concentration and cytoskeletal inhibitors.

Authors: Jessica E Kim; Daniel S Reynolds; Muhammad H Zaman; Michael Mak
Journal: Integr Biol (Camb) Date: 2018-04-23 Impact factor: 2.192

Five challenges to bringing single-molecule force spectroscopy into living cells.

Review 1. Grabbing the cat by the tail: manipulating molecules one by one.

Review 2. Probing the relation between force--lifetime--and chemistry in single molecular bonds.

3. Massively parallel manipulation of single cells and microparticles using optical images.

4. How the doors to the nanoworld were opened.

Review 5. The molecular sociology of the cell.

Review 6. Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy.

Review 7. Structure and mechanics of membrane proteins.

Review 8. A practical guide to single-molecule FRET.

9. Consequences of motor copy number on the intracellular transport of kinesin-1-driven lipid droplets.

10. Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics.

1. Analyzing the micromechanics of the cell division apparatus.

2. Characterization of the mechanical properties of cancer cells in 3D matrices in response to collagen concentration and cytoskeletal inhibitors.

3. Forces in yeast flocculation.

Review 4. Nanoscale monitoring of drug actions on cell membrane using atomic force microscopy.

Review 5. Force probing of individual molecules inside the living cell is now a reality.

Review 6. Atomic force microscopy - looking at mechanosensors on the cell surface.

7. Pulling on single molecules.

Review 8. Studying genomic processes at the single-molecule level: introducing the tools and applications.

9. Artificially-induced organelles are optimal targets for optical trapping experiments in living cells.

10. Probing the stochastic, motor-driven properties of the cytoplasm using force spectrum microscopy.