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Literature DB >> 18511917

Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy.

Abstract

Single-molecule force spectroscopy has emerged as a powerful tool to investigate the forces and motions associated with biological molecules and enzymatic activity. The most common force spectroscopy techniques are optical tweezers, magnetic tweezers and atomic force microscopy. Here we describe these techniques and illustrate them with examples highlighting current capabilities and limitations.

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Neodymium

Year: 2008 PMID： 18511917 PMCID： PMC3397402 DOI： 10.1038/nmeth.1218

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

145 in total

1. Cadherin interaction probed by atomic force microscopy.

Authors: W Baumgartner; P Hinterdorfer; W Ness; A Raab; D Vestweber; H Schindler; D Drenckhahn
Journal: Proc Natl Acad Sci U S A Date: 2000-04-11 Impact factor: 11.205

2. Application of optical traps in vivo.

Authors: Steven P Gross
Journal: Methods Enzymol Date: 2003 Impact factor: 1.600

Review 3. Single-molecule folding.

Authors: Xiaowei Zhuang; Matthias Rief
Journal: Curr Opin Struct Biol Date: 2003-02 Impact factor: 6.809

Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy.

1. Cadherin interaction probed by atomic force microscopy.

2. Application of optical traps in vivo.

Review 3. Single-molecule folding.

4. Pulling geometry defines the mechanical resistance of a beta-sheet protein.

5. Atomic force microscope.

6. Friction and torque govern the relaxation of DNA supercoils by eukaryotic topoisomerase IB.

7. Single-molecule DNA nanomanipulation: improved resolution through use of shorter DNA fragments.

8. Molecular basis of fibrin clot elasticity.

9. Direct observation of kinesin stepping by optical trapping interferometry.

10. Elasticity and unfolding of single molecules of the giant muscle protein titin.

1. Focal Adhesion Induction at the Tip of a Functionalized Nanoelectrode.

2. A hot-spot motif characterizes the interface between a designed ankyrin-repeat protein and its target ligand.

3. Site-specific fluorescent labeling and oriented immobilization of a triple mutant of CYP3A4 via C64.

4. Unraveling the hydrophobic effect, one molecule at a time.

5. Hydrodynamic trapping of molecules in lipid bilayers.

6. Non-bias-limited tracking of spherical particles, enabling nanometer resolution at low magnification.

7. Manipulating particle trajectories with phase-control in surface acoustic wave microfluidics.

8. On-chip manipulation of single microparticles, cells, and organisms using surface acoustic waves.

9. Mesoscopic models for DNA stretching under force: New results and comparison with experiments.

10. Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy.