Literature DB >> 21272647

Retrofit implementation of Zernike phase plate imaging for cryo-TEM.

Michael Marko1, Ardean Leith, Chyongere Hsieh, Radostin Danev.   

Abstract

In-focus phase-plate imaging is particularly beneficial for cryo-TEM because it offers a substantial overall increase in image contrast, without an electron dose penalty, and it simplifies image interpretation. We show how phase-plate cryo-TEM can be implemented with an appropriate existing TEM, and provide a basic practical introduction to use of thin-film (carbon) phase plates. We point out potential pitfalls of phase-plate operation, and discuss solutions. We provide information on evaluating a particular TEM for its suitability.
Copyright © 2011 Elsevier Inc. All rights reserved.

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Year:  2011        PMID： 21272647      PMCID： PMC3078180          DOI： 10.1016/j.jsb.2011.01.005

Source DB:  PubMed          Journal:  J Struct Biol        ISSN： 1047-8477            Impact factor:   2.867


  32 in total

1.  Transmission electron microscopy with Zernike phase plate.

Authors:  R Danev; K Nagayama
Journal:  Ultramicroscopy       Date:  2001-09       Impact factor: 2.689

2.  Electric charging of thin films measured using the contrast transfer function.

Authors:  K Danov; R Danev; K Nagayama
Journal:  Ultramicroscopy       Date:  2001-03       Impact factor: 2.689

3.  Phase plates for transmission electron microscopy.

Authors:  Radostin Danev; Kuniaki Nagayama
Journal:  Methods Enzymol       Date:  2010       Impact factor: 1.600

4.  Zernike phase contrast electron microscopy of ice-embedded influenza A virus.

Authors:  Masashi Yamaguchi; Radostin Danev; Kiyoto Nishiyama; Keishin Sugawara; Kuniaki Nagayama
Journal:  J Struct Biol       Date:  2008-01-26       Impact factor: 2.867

5.  Improved specimen reconstruction by Hilbert phase contrast tomography.

Authors:  Bastian Barton; Friederike Joos; Rasmus R Schröder
Journal:  J Struct Biol       Date:  2008-08-07       Impact factor: 2.867

6.  Phase TEM for biological imaging utilizing a Boersch electrostatic phase plate: theory and practice.

Authors:  Jessie Shiue; Chia-Seng Chang; Sen-Hui Huang; Chih-Hao Hsu; Jin-Sheng Tsai; Wei-Hau Chang; Yi-Min Wu; Yen-Chen Lin; Pai-Chia Kuo; Yang-Shan Huang; Yeukuang Hwu; Ji-Jung Kai; Fan-Gang Tseng; Fu-Rong Chen
Journal:  J Electron Microsc (Tokyo)       Date:  2009-03-16

7.  A Novel Phase-contrast Transmission Electron Microscopy Producing High-contrast Topographic Images of Weak objects.

Authors:  R Danev; H Okawara; N Usuda; K Kametani; K Nagayama
Journal:  J Biol Phys       Date:  2002-12       Impact factor: 1.365

8.  Design of an electron microscope phase plate using a focused continuous-wave laser.

Authors:  H Müller; Jian Jin; R Danev; J Spence; H Padmore; R M Glaeser
Journal:  New J Phys       Date:  2010-07       Impact factor: 3.729

9.  JADAS: a customizable automated data acquisition system and its application to ice-embedded single particles.

Authors:  Junjie Zhang; Natsuko Nakamura; Yuko Shimizu; Nathan Liang; Xiangan Liu; Joanita Jakana; Michael P Marsh; Christopher R Booth; Takao Shinkawa; Munetaka Nakata; Wah Chiu
Journal:  J Struct Biol       Date:  2008-09-30       Impact factor: 2.867

10.  Zernike phase contrast cryo-electron tomography.

Authors:  Radostin Danev; Shuji Kanamaru; Michael Marko; Kuniaki Nagayama
Journal:  J Struct Biol       Date:  2010-03-27       Impact factor: 2.867
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  8 in total

1.  Methods for testing Zernike phase plates and a report on silicon-based phase plates with reduced charging and improved ageing characteristics.

Authors:  Michael Marko; Xing Meng; Chyongere Hsieh; James Roussie; Christopher Striemer
Journal:  J Struct Biol       Date:  2013-08-28       Impact factor: 2.867

2.  Generalization of the Matsumoto-Tonomura approximation for the phase shift within an open aperture.

Authors:  Robert M Glaeser; Holger Müller
Journal:  Ultramicroscopy       Date:  2013-11-28       Impact factor: 2.689

Review 3.  Invited review article: Methods for imaging weak-phase objects in electron microscopy.

Authors:  Robert M Glaeser
Journal:  Rev Sci Instrum       Date:  2013-11       Impact factor: 1.523

4.  Practical Experience with Hole-Free Phase Plates for Cryo Electron Microscopy.

Authors:  Michael Marko; Chyongere Hsieh; Eric Leith; David Mastronarde; Sohei Motoki
Journal:  Microsc Microanal       Date:  2016-11-24       Impact factor: 4.127

5.  Design of a hybrid double-sideband/single-sideband (schlieren) objective aperture suitable for electron microscopy.

Authors:  Bart Buijsse; Frank M H M van Laarhoven; Andreas K Schmid; Rossana Cambie; Stefano Cabrini; Jian Jin; Robert M Glaeser
Journal:  Ultramicroscopy       Date:  2011-10-04       Impact factor: 2.689

6.  Zernike phase-contrast electron cryotomography applied to marine cyanobacteria infected with cyanophages.

Authors:  Wei Dai; Caroline Fu; Htet A Khant; Steven J Ludtke; Michael F Schmid; Wah Chiu
Journal:  Nat Protoc       Date:  2014-10-16       Impact factor: 13.491

7.  Minimizing electrostatic charging of an aperture used to produce in-focus phase contrast in the TEM.

Authors:  Robert M Glaeser; Simone Sassolini; Rossana Cambie; Jian Jin; Stefano Cabrini; Andreas K Schmid; Radostin Danev; Bart Buijsse; Roseann Csencsits; Kenneth H Downing; David M Larson; Dieter Typke; B G Han
Journal:  Ultramicroscopy       Date:  2013-06-08       Impact factor: 2.689

8.  Visualizing virus assembly intermediates inside marine cyanobacteria.

Authors:  Wei Dai; Caroline Fu; Desislava Raytcheva; John Flanagan; Htet A Khant; Xiangan Liu; Ryan H Rochat; Cameron Haase-Pettingell; Jacqueline Piret; Steve J Ludtke; Kuniaki Nagayama; Michael F Schmid; Jonathan A King; Wah Chiu
Journal:  Nature       Date:  2013-10-09       Impact factor: 49.962
  8 in total

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