Literature DB >> 21221452

Bioorthogonal chemical tagging of protein cholesterylation in living cells.

William P Heal1, Biljana Jovanovic, Sara Bessin, Megan H Wright, Anthony I Magee, Edward W Tate.   

Abstract

We report the first chemical probe for bioorthogonal chemical tagging of post-translationally cholesterylated proteins with an azide in living cells. This enables rapid multiplexed fluorescence detection and affinity labelling of protein cholesterylation, as exemplified by Sonic hedgehog protein, opening up new approaches for the de novo identification of cholesterylated proteins.

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Year:  2011        PMID： 21221452     DOI： 10.1039/c0cc04710d

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  37 in total

1.  Multifunctional protein labeling via enzymatic N-terminal tagging and elaboration by click chemistry.

Authors:  William P Heal; Megan H Wright; Emmanuelle Thinon; Edward W Tate
Journal:  Nat Protoc       Date:  2011-12-22       Impact factor: 13.491

Review 2.  Exploring protein lipidation with chemical biology.

Authors:  Howard C Hang; Maurine E Linder
Journal:  Chem Rev       Date:  2011-09-16       Impact factor: 60.622

3.  Bioorthogonal chemical reporters for analyzing protein lipidation and lipid trafficking.

Authors:  Howard C Hang; John P Wilson; Guillaume Charron
Journal:  Acc Chem Res       Date:  2011-06-15       Impact factor: 22.384

4.  Single-cell in situ imaging of palmitoylation in fatty-acylated proteins.

Authors:  Xinxin Gao; Rami N Hannoush
Journal:  Nat Protoc       Date:  2014-10-09       Impact factor: 13.491

5.  A novel alkyne cholesterol to trace cellular cholesterol metabolism and localization.

Authors:  Kristina Hofmann; Christoph Thiele; Hans-Frieder Schött; Anne Gaebler; Mario Schoene; Yuriy Kiver; Silvia Friedrichs; Dieter Lütjohann; Lars Kuerschner
Journal:  J Lipid Res       Date:  2013-12-12       Impact factor: 5.922

6.  Protein Lipidation: Occurrence, Mechanisms, Biological Functions, and Enabling Technologies.

Authors:  Hong Jiang; Xiaoyu Zhang; Xiao Chen; Pornpun Aramsangtienchai; Zhen Tong; Hening Lin
Journal:  Chem Rev       Date:  2018-01-02       Impact factor: 60.622

7.  Protein-Nucleic Acid Conjugation with Sterol Linkers Using Hedgehog Autoprocessing.

Authors:  Xiaoyu Zhang; Zihan Xu; Dina S Moumin; Daniel A Ciulla; Timothy S Owen; Rebecca A Mancusi; José-Luis Giner; Chunyu Wang; Brian P Callahan
Journal:  Bioconjug Chem       Date:  2019-10-10       Impact factor: 4.774

8.  Active site targeting of hedgehog precursor protein with phenylarsine oxide.

Authors:  Timothy S Owen; Xie Jian Xie; Benjamin Laraway; George Ngoje; Chunyu Wang; Brian P Callahan
Journal:  Chembiochem       Date:  2014-11-21       Impact factor: 3.164

Review 9.  Pharmacological Inhibition of Protein Lipidation.

Authors:  Lakshmi Ganesan; Ilya Levental
Journal:  J Membr Biol       Date:  2015-08-18       Impact factor: 1.843

Review 10.  Chemical reporters for biological discovery.

Authors:  Markus Grammel; Howard C Hang
Journal:  Nat Chem Biol       Date:  2013-08       Impact factor: 15.040
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