Literature DB >> 21190673

Detection of membrane protein two-dimensional crystals in living cells.

E J Gualtieri1, F Guo, D J Kissick, J Jose, R J Kuhn, W Jiang, G J Simpson.   

Abstract

It is notoriously difficult to grow membrane protein crystals and solve membrane protein structures. Improved detection and screening of membrane protein crystals are needed. We have shown here that second-order nonlinear optical imaging of chiral crystals based on second harmonic generation can provide sensitive and selective detection of two-dimensional protein crystalline arrays with sufficiently low background to enable crystal detection within the membranes of live cells. The method was validated using bacteriorhodopsin crystals generated in live Halobacterium halobium bacteria and confirmed by electron microscopy from the isolated crystals. Additional studies of alphavirus glycoproteins indicated the presence of localized crystalline domains associated with virus budding from mammalian cells. These results suggest that in vivo crystallization may provide a means for expediting membrane protein structure determination for proteins exhibiting propensities for two-dimensional crystal formation.
Copyright © 2011 Biophysical Society. Published by Elsevier Inc. All rights reserved.

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Year:  2011        PMID： 21190673      PMCID： PMC3010839          DOI： 10.1016/j.bpj.2010.10.051

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  45 in total

1.  Nicotinic acetylcholine receptor at 4.6 A resolution: transverse tunnels in the channel wall.

Authors:  A Miyazawa; Y Fujiyoshi; M Stowell; N Unwin
Journal:  J Mol Biol       Date:  1999-05-14       Impact factor: 5.469

2.  2dx--user-friendly image processing for 2D crystals.

Authors:  Bryant Gipson; Xiangyan Zeng; Zi Yan Zhang; Henning Stahlberg
Journal:  J Struct Biol       Date:  2006-09-01       Impact factor: 2.867

3.  Towards automated screening of two-dimensional crystals.

Authors:  Anchi Cheng; Albert Leung; Denis Fellmann; Joel Quispe; Christian Suloway; James Pulokas; Priyanka D Abeyrathne; Joseph S Lam; Bridget Carragher; Clinton S Potter
Journal:  J Struct Biol       Date:  2007-09-25       Impact factor: 2.867

4.  Overcoming photodamage in second-harmonic generation microscopy: real-time optical recording of neuronal action potentials.

Authors:  L Sacconi; D A Dombeck; W W Webb
Journal:  Proc Natl Acad Sci U S A       Date:  2006-02-17       Impact factor: 11.205

5.  The mercurial insensitive water channel (AQP-4) forms orthogonal arrays in stably transfected Chinese hamster ovary cells.

Authors:  B Yang; D Brown; A S Verkman
Journal:  J Biol Chem       Date:  1996-03-01       Impact factor: 5.157

6.  Atomic model of plant light-harvesting complex by electron crystallography.

Authors:  W Kühlbrandt; D N Wang; Y Fujiyoshi
Journal:  Nature       Date:  1994-02-17       Impact factor: 49.962

7.  Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy.

Authors:  R Henderson; J M Baldwin; T A Ceska; F Zemlin; E Beckmann; K H Downing
Journal:  J Mol Biol       Date:  1990-06-20       Impact factor: 5.469

8.  Structural basis for induced formation of the inflammatory mediator prostaglandin E2.

Authors:  Caroline Jegerschöld; Sven-Christian Pawelzik; Pasi Purhonen; Priyaranjan Bhakat; Karina Roxana Gheorghe; Nobuhiko Gyobu; Kaoru Mitsuoka; Ralf Morgenstern; Per-Johan Jakobsson; Hans Hebert
Journal:  Proc Natl Acad Sci U S A       Date:  2008-08-05       Impact factor: 11.205

Review 9.  Milestones in electron crystallography.

Authors:  Ludovic Renault; Hui-Ting Chou; Po-Lin Chiu; Rena M Hill; Xiangyan Zeng; Bryant Gipson; Zi Yan Zhang; Anchi Cheng; Vinzenz Unger; Henning Stahlberg
Journal:  J Comput Aided Mol Des       Date:  2006-11-11       Impact factor: 3.686

10.  A tyrosine-based motif in the cytoplasmic domain of the alphavirus envelope protein is essential for budding.

Authors:  H Zhao; B Lindqvist; H Garoff; C H von Bonsdorff; P Liljeström
Journal:  EMBO J       Date:  1994-09-15       Impact factor: 11.598
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  9 in total

Review 1.  Femtosecond nanocrystallography using X-ray lasers for membrane protein structure determination.

Authors:  Petra Fromme; John C H Spence
Journal:  Curr Opin Struct Biol       Date:  2011-08       Impact factor: 6.809

2.  Two-photon excited UV fluorescence for protein crystal detection.

Authors:  Jeremy T Madden; Emma L DeWalt; Garth J Simpson
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2011-09-08

3.  Probing the early temporal and spatial interaction of the Sindbis virus capsid and E2 proteins with reverse genetics.

Authors:  Jonathan E Snyder; Christian J Berrios; Thomas J Edwards; Joyce Jose; Rushika Perera; Richard J Kuhn
Journal:  J Virol       Date:  2012-09-05       Impact factor: 5.103

4.  Modeling the SHG activities of diverse protein crystals.

Authors:  Levi M Haupert; Emma L DeWalt; Garth J Simpson
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2012-10-18

5.  Towards protein-crystal centering using second-harmonic generation (SHG) microscopy.

Authors:  David J Kissick; Christopher M Dettmar; Michael Becker; Anne M Mulichak; Vadim Cherezov; Stephan L Ginell; Kevin P Battaile; Lisa J Keefe; Robert F Fischetti; Garth J Simpson
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2013-04-19

6.  Cell-to-cell diversity in a synchronized Chlamydomonas culture as revealed by single-cell analyses.

Authors:  Andreas Garz; Michael Sandmann; Michael Rading; Sascha Ramm; Ralf Menzel; Martin Steup
Journal:  Biophys J       Date:  2012-09-05       Impact factor: 4.033

7.  Second harmonic generation correlation spectroscopy for characterizing translationally diffusing protein nanocrystals.

Authors:  Ximeng Y Dow; Christopher M Dettmar; Emma L DeWalt; Justin A Newman; Alexander R Dow; Shatabdi Roy-Chowdhury; Jesse D Coe; Christopher Kupitz; Petra Fromme; Garth J Simpson
Journal:  Acta Crystallogr D Struct Biol       Date:  2016-06-23       Impact factor: 7.652

Review 8.  Membrane protein structure determination by electron crystallography.

Authors:  Iban Ubarretxena-Belandia; David L Stokes
Journal:  Curr Opin Struct Biol       Date:  2012-05-08       Impact factor: 6.809

9.  In cellulo serial crystallography of alcohol oxidase crystals inside yeast cells.

Authors:  Arjen J Jakobi; Daniel M Passon; Kèvin Knoops; Francesco Stellato; Mengning Liang; Thomas A White; Thomas Seine; Marc Messerschmidt; Henry N Chapman; Matthias Wilmanns
Journal:  IUCrJ       Date:  2016-01-12       Impact factor: 4.769
  9 in total

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