Literature DB >> 21185880

SOE-LRed: A simple and time-efficient method to localize genes with point mutations onto the Escherichia coli chromosome.

Ryan W Benson1, Tiziana M Cafarelli, Veronica G Godoy.   

Abstract

We use a powerful method to replace wild-type genes on the chromosome of Escherichia coli. Using a unique form of PCR, we generate easily constructible gene fusions bearing single point mutations. Used in conjunction with homologous recombination, this method eliminates cloning procedures previously used for this purpose.
Copyright © 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID： 21185880      PMCID： PMC3045631          DOI： 10.1016/j.mimet.2010.12.020

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  9 in total

1.  One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.

Authors:  K A Datsenko; B L Wanner
Journal:  Proc Natl Acad Sci U S A       Date:  2000-06-06       Impact factor: 11.205

2.  Chromosome and low copy plasmid segregation in E. coli: visual evidence for distinct mechanisms.

Authors:  G S Gordon; D Sitnikov; C D Webb; A Teleman; A Straight; R Losick; A W Murray; A Wright
Journal:  Cell       Date:  1997-09-19       Impact factor: 41.582

3.  Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.

Authors:  R M Horton; H D Hunt; S N Ho; J K Pullen; L R Pease
Journal:  Gene       Date:  1989-04-15       Impact factor: 3.688

4.  The dinB gene encodes a novel E. coli DNA polymerase, DNA pol IV, involved in mutagenesis.

Authors:  J Wagner; P Gruz; S R Kim; M Yamada; K Matsui; R P Fuchs; T Nohmi
Journal:  Mol Cell       Date:  1999-08       Impact factor: 17.970

5.  Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization.

Authors:  A J Link; D Phillips; G M Church
Journal:  J Bacteriol       Date:  1997-10       Impact factor: 3.490

6.  PCR-mediated gene replacement in Escherichia coli.

Authors:  K C Murphy; K G Campellone; A R Poteete
Journal:  Gene       Date:  2000-04-04       Impact factor: 3.688

7.  A DinB variant reveals diverse physiological consequences of incomplete TLS extension by a Y-family DNA polymerase.

Authors:  Daniel F Jarosz; Susan E Cohen; James C Delaney; John M Essigmann; Graham C Walker
Journal:  Proc Natl Acad Sci U S A       Date:  2009-11-30       Impact factor: 11.205

8.  Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection.

Authors:  Tomoya Baba; Takeshi Ara; Miki Hasegawa; Yuki Takai; Yoshiko Okumura; Miki Baba; Kirill A Datsenko; Masaru Tomita; Barry L Wanner; Hirotada Mori
Journal:  Mol Syst Biol       Date:  2006-02-21       Impact factor: 11.429

9.  Simple generation of site-directed point mutations in the Escherichia coli chromosome using Red(R)/ET(R) Recombination.

Authors:  Ralf Heermann; Tim Zeppenfeld; Kirsten Jung
Journal:  Microb Cell Fact       Date:  2008-04-24       Impact factor: 5.328
  9 in total
  6 in total

1.  A single residue unique to DinB-like proteins limits formation of the polymerase IV multiprotein complex in Escherichia coli.

Authors:  Tiziana M Cafarelli; Thomas J Rands; Ryan W Benson; Pamela A Rudnicki; Ida Lin; Veronica G Godoy
Journal:  J Bacteriol       Date:  2013-01-04       Impact factor: 3.490

2.  Selection of dinB alleles suppressing survival loss upon dinB overexpression in Escherichia coli.

Authors:  Ryan W Benson; Tiziana M Cafarelli; Thomas J Rands; Ida Lin; Veronica G Godoy
Journal:  J Bacteriol       Date:  2014-06-09       Impact factor: 3.490

3.  Activation of phenotypic subpopulations in response to ciprofloxacin treatment in Acinetobacter baumannii.

Authors:  Ashley E Macguire; Meining Carly Ching; Brett H Diamond; Alexey Kazakov; Pavel Novichkov; Veronica G Godoy
Journal:  Mol Microbiol       Date:  2014-03-03       Impact factor: 3.501

4.  Structure of Escherichia coli dGTP triphosphohydrolase: a hexameric enzyme with DNA effector molecules.

Authors:  Deepa Singh; Damian Gawel; Mark Itsko; Alejandro Hochkoeppler; Juno M Krahn; Robert E London; Roel M Schaaper
Journal:  J Biol Chem       Date:  2015-02-18       Impact factor: 5.157

5.  An active site aromatic triad in Escherichia coli DNA Pol IV coordinates cell survival and mutagenesis in different DNA damaging agents.

Authors:  Ryan W Benson; Matthew D Norton; Ida Lin; William S Du Comb; Veronica G Godoy
Journal:  PLoS One       Date:  2011-05-17       Impact factor: 3.240

6.  A Genetic Selection for dinB Mutants Reveals an Interaction between DNA Polymerase IV and the Replicative Polymerase That Is Required for Translesion Synthesis.

Authors:  Michelle K Scotland; Justin M H Heltzel; James E Kath; Jung-Suk Choi; Anthony J Berdis; Joseph J Loparo; Mark D Sutton
Journal:  PLoS Genet       Date:  2015-09-09       Impact factor: 5.917
  6 in total

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