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Literature DB >> 21142151

A photocleavable rapamycin conjugate for spatiotemporal control of small GTPase activity.

Nobuhiro Umeda¹, Tasuku Ueno, Christopher Pohlmeyer, Tetsuo Nagano, Takanari Inoue.

Abstract

We developed a novel method to spatiotemporally control the activity of signaling molecules. A newly synthesized photocaged rapamycin derivative induced rapid dimerization of FKBP (FK-506 binding protein) and FRB (FKBP-rapamycin binding protein) upon UV irradiation. With this system and the spatially confined UV irradiation, we achieved subcellularly localized activation of Rac, a member of small GTPases. Our technique offers a powerful approach to studies of dynamic intracellular signaling events.

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Year: 2010 PMID： 21142151 PMCID： PMC3850177 DOI： 10.1021/ja108258d

Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419

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