Literature DB >> 33481234

Induced Dimerization Tools to Deplete Specific Phosphatidylinositol Phosphates.

Jonathan Pacheco1, Rachel C Wills1, Gerald R V Hammond2.   

Abstract

Chemical dimerization systems have been used to drive acute depletion of polyphosphoinsitides (PPIns). They do so by inducing subcellular localization of enzymes that catabolize PPIns. By using this approach, all seven PPIns can be depleted in living cells and in real time. The rapid permeation of dimerizer agents and the specific expression of recruiter proteins confer great spatial and temporal resolution with minimal cell perturbation. In this chapter, we provide detailed instructions to monitor and induce depletion of PPIns in live cells.

Entities:  

Keywords:  Dimerization; Kinase; Lipid-binding domain; Phosphatase; Rapamycin

Year:  2021        PMID： 33481234     DOI： 10.1007/978-1-0716-1142-5_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  49 in total

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Authors:  Robert DeRose; Takafumi Miyamoto; Takanari Inoue
Journal:  Pflugers Arch       Date:  2013-01-09       Impact factor: 3.657

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Authors:  Eamonn J Dickson; Bertil Hille
Journal:  Biochem J       Date:  2019-01-07       Impact factor: 3.857

6.  Rapamycin analogs with differential binding specificity permit orthogonal control of protein activity.

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Journal:  Chem Biol       Date:  2006-01

7.  FRAP/mTOR is required for proliferation and patterning during embryonic development in the mouse.

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Journal:  Proc Natl Acad Sci U S A       Date:  2001-11-13       Impact factor: 11.205

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Journal:  Proc Natl Acad Sci U S A       Date:  1995-05-23       Impact factor: 11.205

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Journal:  Nature       Date:  1994-06-30       Impact factor: 49.962

10.  Genetically encoded lipid biosensors.

Authors:  Rachel C Wills; Brady D Goulden; Gerald R V Hammond
Journal:  Mol Biol Cell       Date:  2018-07-01       Impact factor: 4.138
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