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Literature DB >> 21136327

An HNCO-based Pulse Scheme for the Measurement of 13Cα-1Hα One-bond Dipolar couplings in 15N, 13C Labeled Proteins.

Abstract

A triple resonance pulse scheme is presented for recording 13Cα-1Hα one-bond dipolar couplings in 15N, 13C labeled proteins. HNCO correlation maps are generated where the carbonyl chemical shift is modulated by the 13Cα-1Hα coupling, with the two doublet components separated into individual data sets. The experiment makes use of recently described methodology whereby the protein of interest is dissolved in a dilute solution of bicelles which orient above a critical temperature, thus permitting measurement of significant couplings (Tjandra and Bax, 1997a). An application to the protein ubiquitin is described.

Entities: Chemical Gene

Year: 1998 PMID： 21136327 DOI： 10.1023/A:1008223017233

Source DB: PubMed Journal: J Biomol NMR ISSN： 0925-2738 Impact factor: 2.835

13 in total

1. Measurement of dipolar contributions to 1JCH splittings from magnetic-field dependence of J modulation in two-dimensional NMR spectra.

Authors: N Tjandra; A Bax
Journal: J Magn Reson Date: 1997-02 Impact factor: 2.229

2. NMR evidence for slow collective motions in cyanometmyoglobin.

Authors: J R Tolman; J M Flanagan; M A Kennedy; J H Prestegard
Journal: Nat Struct Biol Date: 1997-04

3. Structure of ubiquitin refined at 1.8 A resolution.

Authors: S Vijay-Kumar; C E Bugg; W J Cook
Journal: J Mol Biol Date: 1987-04-05 Impact factor: 5.469

4. A quantitative J-correlation experiment for the accurate measurement of one-bond amide 15N-1H couplings in proteins.

Authors: J R Tolman; J H Prestegard
Journal: J Magn Reson B Date: 1996-09

5. Measurement of amide 15N-1H one-bond couplings in proteins using accordion heteronuclear-shift-correlation experiments.

Authors: J R Tolman; J H Prestegard
Journal: J Magn Reson B Date: 1996-09

6. NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

Authors: F Delaglio; S Grzesiek; G W Vuister; G Zhu; J Pfeifer; A Bax
Journal: J Biomol NMR Date: 1995-11 Impact factor: 2.835

7. Amino acid type determination in the sequential assignment procedure of uniformly 13C/15N-enriched proteins.

Authors: S Grzesiek; A Bax
Journal: J Biomol NMR Date: 1993-03 Impact factor: 2.835

8. A general method for assigning NMR spectra of denatured proteins using 3D HC(CO)NH-TOCSY triple resonance experiments.

Authors: T M Logan; E T Olejniczak; R X Xu; S W Fesik
Journal: J Biomol NMR Date: 1993-03 Impact factor: 2.835

9. Characterization of magnetically orientable bilayers in mixtures of dihexanoylphosphatidylcholine and dimyristoylphosphatidylcholine by solid-state NMR.

Authors: C R Sanders; J P Schwonek
Journal: Biochemistry Date: 1992-09-22 Impact factor: 3.162

10. Nuclear magnetic dipole interactions in field-oriented proteins: information for structure determination in solution.

Authors: J R Tolman; J M Flanagan; M A Kennedy; J H Prestegard
Journal: Proc Natl Acad Sci U S A Date: 1995-09-26 Impact factor: 11.205

20 in total

1. A set of HA-detected experiments for measuring scalar and residual dipolar couplings.

Authors: Peter Würtz; Kai Fredriksson; Perttu Permi
Journal: J Biomol NMR Date: 2005-04 Impact factor: 2.835

2. J-Spectroscopy in the presence of residual dipolar couplings: determination of one-bond coupling constants and scalable resolution.

Authors: Julien Furrer; Michael John; Horst Kessler; Burkhard Luy
Journal: J Biomol NMR Date: 2007-01-19 Impact factor: 2.835

3. Solution structure of the Ubp-M BUZ domain, a highly specific protein module that recognizes the C-terminal tail of free ubiquitin.

Authors: Ming-Tao Pai; Shiou-Ru Tzeng; Jeffrey J Kovacs; Mignon A Keaton; Shawn S-C Li; Tso-Pang Yao; Pei Zhou
Journal: J Mol Biol Date: 2007-04-12 Impact factor: 5.469

4. NMR-based homology model for the solution structure of the C-terminal globular domain of EMILIN1.

Authors: Giuliana Verdone; Alessandra Corazza; Simon A Colebrooke; Daniel Cicero; Tommaso Eliseo; Jonathan Boyd; Roberto Doliana; Federico Fogolari; Paolo Viglino; Alfonso Colombatti; Iain D Campbell; Gennaro Esposito
Journal: J Biomol NMR Date: 2008-11-21 Impact factor: 2.835

5. Structure of tumor suppressor p53 and its intrinsically disordered N-terminal transactivation domain.

Authors: Mark Wells; Henning Tidow; Trevor J Rutherford; Phineus Markwick; Malene Ringkjobing Jensen; Efstratios Mylonas; Dmitri I Svergun; Martin Blackledge; Alan R Fersht
Journal: Proc Natl Acad Sci U S A Date: 2008-04-07 Impact factor: 11.205

6. Improved dilute bicelle solutions for high-resolution NMR of biological macromolecules.

Authors: J A Losonczi; J H Prestegard
Journal: J Biomol NMR Date: 1998-10 Impact factor: 2.835

7. A spin-state-selective experiment for measuring heteronuclear one-bond and homonuclear two-bond couplings from an HSQC-type spectrum.

Authors: Perttu Permi
Journal: J Biomol NMR Date: 2002-01 Impact factor: 2.835

8. Measurement of residual dipolar couplings from 1Halpha to 13Calpha and 15N using a simple HNCA-based experiment.

Authors: Perttu Permi
Journal: J Biomol NMR Date: 2003-12 Impact factor: 2.835

9. The solution structure of the Mg2+ form of soybean calmodulin isoform 4 reveals unique features of plant calmodulins in resting cells.

Authors: Hao Huang; Hiroaki Ishida; Hans J Vogel
Journal: Protein Sci Date: 2010-03 Impact factor: 6.725

10. Residual dipolar coupling measurements of transmembrane proteins using aligned low-q bicelles and high-resolution magic angle spinning NMR spectroscopy.

Authors: Christian G Canlas; Dejian Ma; Pei Tang; Yan Xu
Journal: J Am Chem Soc Date: 2008-09-13 Impact factor: 15.419