Literature DB >> 1370345

Three-dimensional heteronuclear NMR studies of RNA.

E P Nikonowicz1, A Pardi.   

Abstract

Multidimensional heteronuclear NMR has revolutionized solution structure determinations of proteins. But this technique has not been applied to nucleic acids because of difficulties in the synthesis of isotopically (13C and/or 15N) labelled molecules. Here we report the application of three-dimensional heteronuclear NMR to the study of a uniformly 13C/15N or 15N-labelled RNA duplex of defined sequence. These experiments simplify resonance assignment and the analysis of proton-proton nuclear Overhauser effects (and therefore distance information) in the molecule. Our results show that it is now possible to determine the structures of larger and more complex RNAs using multidimensional heteronuclear NMR.

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Year:  1992        PMID： 1370345     DOI： 10.1038/355184a0

Source DB:  PubMed          Journal:  Nature        ISSN： 0028-0836            Impact factor:   49.962


  29 in total

1.  Superior 5' homogeneity of RNA from ATP-initiated transcription under the T7 phi 2.5 promoter.

Authors:  Tricia M Coleman; Guocan Wang; Faqing Huang
Journal:  Nucleic Acids Res       Date:  2004-01-15       Impact factor: 16.971

2.  Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants.

Authors:  P Schmieder; J H Ippel; H van den Elst; G A van der Marel; J H van Boom; C Altona; H Kessler
Journal:  Nucleic Acids Res       Date:  1992-09-25       Impact factor: 16.971

3.  A New Experiment for the Measurement of nJ(C,P) Coupling Constants Including 3J(C4'i,Pi) and 3J(C4'i,Pi+1) in Oligonucleotides.

Authors:  C Richter; B Reif; K Wörner; S Quant; J P Marino; J W Engels; C Griesinger; H Schwalbe
Journal:  J Biomol NMR       Date:  1998-08       Impact factor: 2.835

4.  A genetic algorithm based molecular modeling technique for RNA stem-loop structures.

Authors:  H Ogata; Y Akiyama; M Kanehisa
Journal:  Nucleic Acids Res       Date:  1995-02-11       Impact factor: 16.971

5.  Visualization of RNA tertiary structure by RNA-EDTA.Fe(II) autocleavage: analysis of tRNA(Phe) with uridine-EDTA.Fe(II) at position 47.

Authors:  H Han; P B Dervan
Journal:  Proc Natl Acad Sci U S A       Date:  1994-05-24       Impact factor: 11.205

6.  The differences in the T2 relaxation rates of the protons in the partially-deuteriated and fully protonated sugar residues in a large oligo-DNA ('NMR-window') gives complementary structural information.

Authors:  P Agback; T V Maltseva; S I Yamakage; F P Nilson; A Földesi; J Chattopadhyaya
Journal:  Nucleic Acids Res       Date:  1994-04-25       Impact factor: 16.971

7.  Preparation of 13C and 15N labelled RNAs for heteronuclear multi-dimensional NMR studies.

Authors:  E P Nikonowicz; A Sirr; P Legault; F M Jucker; L M Baer; A Pardi
Journal:  Nucleic Acids Res       Date:  1992-09-11       Impact factor: 16.971

8.  Synthesis of backbone deuterium labelled [r(CGCGAAUUCGCG)]2 and HPLC purification of synthetic RNA.

Authors:  D Khare; J Orban
Journal:  Nucleic Acids Res       Date:  1992-10-11       Impact factor: 16.971

9.  Preparation of isotopically labeled ribonucleotides for multidimensional NMR spectroscopy of RNA.

Authors:  R T Batey; M Inada; E Kujawinski; J D Puglisi; J R Williamson
Journal:  Nucleic Acids Res       Date:  1992-09-11       Impact factor: 16.971

Review 10.  Isotope labeling strategies for NMR studies of RNA.

Authors:  Kun Lu; Yasuyuki Miyazaki; Michael F Summers
Journal:  J Biomol NMR       Date:  2009-09-30       Impact factor: 2.835
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