Literature DB >> 21114271

Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3' → 5' exonuclease activity.

Chung-Hang Leung, Daniel Shiu-Hin Chan, Bradley Yat-Wah Man, Chuan-Jen Wang, Wing Lam, Yung-Chi Cheng, Wang-Fun Fong, Wen-Luan Wendy Hsiao, Dik-Lung Ma.   

Abstract

A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3' → 5' exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1.

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Year:  2010        PMID： 21114271      PMCID： PMC3021625          DOI： 10.1021/ac1025896

Source DB:  PubMed          Journal:  Anal Chem        ISSN： 0003-2700            Impact factor:   6.986


  27 in total

1.  Human telomeric DNA: G-quadruplex, i-motif and Watson-Crick double helix.

Authors:  Anh Tuân Phan; Jean-Louis Mergny
Journal:  Nucleic Acids Res       Date:  2002-11-01       Impact factor: 16.971

2.  A quadruplex-based, label-free, and real-time fluorescence assay for RNase H activity and inhibition.

Authors:  Dan Hu; Fang Pu; Zhenzhen Huang; Jinsong Ren; Xiaogang Qu
Journal:  Chemistry       Date:  2010-02-22       Impact factor: 5.236

3.  The TREX1 double-stranded DNA degradation activity is defective in dominant mutations associated with autoimmune disease.

Authors:  Duane A Lehtinen; Scott Harvey; Matthew J Mulcahy; Thomas Hollis; Fred W Perrino
Journal:  J Biol Chem       Date:  2008-09-18       Impact factor: 5.157

4.  Synthesis and study of a new adenine-acridine tandem, inhibitor of exonuclease III.

Authors:  P Belmont; M Demeunynck; J F Constant; J Lhomme
Journal:  Bioorg Med Chem Lett       Date:  2000-02-07       Impact factor: 2.823

5.  Abasic site recognition in DNA as a new strategy to potentiate the action of anticancer alkylating drugs?

Authors:  P Belmont; M Jourdan; M Demeunynck; J F Constant; J Garcia; J Lhomme; D Carez; A Croisy
Journal:  J Med Chem       Date:  1999-12-16       Impact factor: 7.446

6.  Discrimination of G-quadruplexes from duplex and single-stranded DNAs with fluorescence and energy-transfer fluorescence spectra of crystal violet.

Authors:  De-Ming Kong; Yong-E Ma; Jing Wu; Han-Xi Shen
Journal:  Chemistry       Date:  2009       Impact factor: 5.236

7.  Structural and biochemical studies of TREX1 inhibition by metals. Identification of a new active histidine conserved in DEDDh exonucleases.

Authors:  Marina Brucet; Jordi Querol-Audí; Kamila Bertlik; Jorge Lloberas; Ignacio Fita; Antonio Celada
Journal:  Protein Sci       Date:  2008-09-09       Impact factor: 6.725

Review 8.  New roles for the major human 3'-5' exonuclease TREX1 in human disease.

Authors:  David Kavanagh; Dirk Spitzer; Parul H Kothari; Aisha Shaikh; M Kathryn Liszewski; Anna Richards; John P Atkinson
Journal:  Cell Cycle       Date:  2008-06-16       Impact factor: 4.534

9.  TREX1 acts in degrading damaged DNA from drug-treated tumor cells.

Authors:  Chuan-Jen Wang; Wing Lam; Scott Bussom; Hua-Mei Chang; Yung-Chi Cheng
Journal:  DNA Repair (Amst)       Date:  2009-07-18

10.  Trex1 prevents cell-intrinsic initiation of autoimmunity.

Authors:  Daniel B Stetson; Joan S Ko; Thierry Heidmann; Ruslan Medzhitov
Journal:  Cell       Date:  2008-08-22       Impact factor: 41.582
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  7 in total

1.  Evidence That G-quadruplex DNA Accumulates in the Cytoplasm and Participates in Stress Granule Assembly in Response to Oxidative Stress.

Authors:  Alicia K Byrd; Boris L Zybailov; Leena Maddukuri; Jun Gao; John C Marecki; Mihir Jaiswal; Matthew R Bell; Wezley C Griffin; Megan R Reed; Shubeena Chib; Samuel G Mackintosh; Angus M MacNicol; Giulia Baldini; Robert L Eoff; Kevin D Raney
Journal:  J Biol Chem       Date:  2016-07-01       Impact factor: 5.157

Review 2.  Fluorescent Probes of DNA Repair.

Authors:  David L Wilson; Eric T Kool
Journal:  ACS Chem Biol       Date:  2017-11-30       Impact factor: 5.100

Review 3.  G-quadruplexes for luminescent sensing and logic gates.

Authors:  Hong-Zhang He; Daniel Shiu-Hin Chan; Chung-Hang Leung; Dik-Lung Ma
Journal:  Nucleic Acids Res       Date:  2013-02-23       Impact factor: 16.971

4.  A highly selective, label-free, homogenous luminescent switch-on probe for the detection of nanomolar transcription factor NF-kappaB.

Authors:  Dik-Lung Ma; Ting Xu; Daniel S-H Chan; Bradley Y-W Man; Wang-Fun Fong; Chung-Hang Leung
Journal:  Nucleic Acids Res       Date:  2011-03-11       Impact factor: 16.971

Review 5.  Luminescent detection of DNA-binding proteins.

Authors:  Chung-Hang Leung; Daniel Shiu-Hin Chan; Hong-Zhang He; Zhen Cheng; Hui Yang; Dik-Lung Ma
Journal:  Nucleic Acids Res       Date:  2011-10-03       Impact factor: 16.971

6.  Characterizations of distinct parallel and antiparallel G-quadruplexes formed by two-repeat ALS and FTD related GGGGCC sequence.

Authors:  Bo Zhou; Yanyan Geng; Changdong Liu; Haitao Miao; Yaguang Ren; Naining Xu; Xiao Shi; Yingying You; Tunglun Lee; Guang Zhu
Journal:  Sci Rep       Date:  2018-02-05       Impact factor: 4.379

7.  An exonuclease III protection-based electrochemical method for estrogen receptor assay.

Authors:  Sha Zhu; Ya Cao; Yuanyuan Xu; Yongmei Yin; Genxi Li
Journal:  Int J Mol Sci       Date:  2013-05-16       Impact factor: 5.923
  7 in total

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