Literature DB >> 2106479

Molecular cloning of DNA from specific chromosomal regions by microdissection and sequence-independent amplification of DNA.

D H Johnson1.   

Abstract

A method that permits the in vitro amplification and cloning of DNA dissected from specific regions of a chromosome and does not require prior knowledge of the DNA sequence is described. DNA from several different chromosomal loci in the Drosophila melanogaster genome has been isolated by this method. Although the procedure was developed to permit the isolation of DNA sequences from serial sections of a single microdissected polytene chromosome, it should be useful for obtaining DNA clones from specific regions of the nonpolytene chromosomes of other organisms as well.

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Year:  1990        PMID： 2106479     DOI： 10.1016/0888-7543(90)90563-a

Source DB:  PubMed          Journal:  Genomics        ISSN： 0888-7543            Impact factor:   5.736


  21 in total

1.  Ligation-independent cloning of PCR products (LIC-PCR).

Authors:  C Aslanidis; P J de Jong
Journal:  Nucleic Acids Res       Date:  1990-10-25       Impact factor: 16.971

2.  Chromosome microdissection and cloning in human genome and genetic disease analysis.

Authors:  F T Kao; J W Yu
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-01       Impact factor: 11.205

3.  Isolation of region-specific cosmids from chromosome 5 by hybridization with microdissection clones.

Authors:  D L Saltman; G M Dolganov; B S Pearce; S S Kuo; P J Callahan; M L Cleary; M Lovett
Journal:  Nucleic Acids Res       Date:  1992-03-25       Impact factor: 16.971

4.  The development of chromosome microdissection and microcloning technique and its applications in genomic research.

Authors:  Ruo-Nan Zhou; Zan-Min Hu
Journal:  Curr Genomics       Date:  2007-03       Impact factor: 2.236

5.  Direct amplification of a single dissected chromosomal segment by polymerase chain reaction: a human brain sodium channel gene is on chromosome 2q22-q23.

Authors:  J A Han; C M Lu; G B Brown; T A Rado
Journal:  Proc Natl Acad Sci U S A       Date:  1991-01-15       Impact factor: 11.205

6.  Disclosure of five breakpoints in a complex chromosome rearrangement by microdissection and FISH.

Authors:  J J Engelen; W J Loots; J C Albrechts; P C Motoh; J P Fryns; A J Hamers; J P Geraedts
Journal:  J Med Genet       Date:  1996-07       Impact factor: 6.318

7.  A novel RNA helicase gene tightly linked to the Triplo-lethal locus of Drosophila.

Authors:  D R Dorer; A C Christensen; D H Johnson
Journal:  Nucleic Acids Res       Date:  1990-09-25       Impact factor: 16.971

8.  Gene mapping from a bovine 1;29 DNA library prepared with chromosome microdissection.

Authors:  S M Schmutz; T G Berryere; J S Moker; T D Thue; D C Winkelman
Journal:  Mamm Genome       Date:  1994-03       Impact factor: 2.957

9.  A region-specific microdissection library for human chromosome 2p23-p25 and the analysis of an interstitial deletion of 2p23.3-p25.1.

Authors:  J Yu; J Qi; S Tong; F T Kao
Journal:  Hum Genet       Date:  1994-05       Impact factor: 4.132

10.  Development of a database system for mapping insertional mutations onto the mouse genome with large-scale experimental data.

Authors:  Wenwei Yang; Ke Jin; Xing Xie; Dongsheng Li; Jigang Yang; Li Wang; Ning Gu; Yang Zhong; Ling V Sun
Journal:  BMC Genomics       Date:  2009-12-03       Impact factor: 3.969
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