Literature DB >> 1846440

Direct amplification of a single dissected chromosomal segment by polymerase chain reaction: a human brain sodium channel gene is on chromosome 2q22-q23.

J A Han1, C M Lu, G B Brown, T A Rado.   

Abstract

We have devised a general strategy for gene mapping based upon the direct amplification of a target sequence within a single microdissected Giemsa-banded chromosomal segment using the polymerase chain reaction. The usefulness of this approach was demonstrated by mapping a cloned human brain sodium channel (alpha subunit) gene sequence to chromosome 2q22-q23. When DNA from single, dissected chromosome segments 2q21-qter and 2q24-pter were used as templates, a sodium channel-specific 172-base-pair polymerase chain reaction product was obtained. This product was not synthesized when segments 2q21-pter and 2q24-qter were used. Chromosome microdissection-polymerase chain reaction is not only a simple, fast, and accurate method for gene mapping but also may offer significant advantages for other applications, such as cancer cytogenetics and linkage analysis.

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Year:  1991        PMID： 1846440      PMCID： PMC50805          DOI： 10.1073/pnas.88.2.335

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  23 in total

1.  Localization of a human brain sodium channel gene (SCN2A) to chromosome 2.

Authors:  M Litt; J Luty; M Kwak; L Allen; R E Magenis; G Mandel
Journal:  Genomics       Date:  1989-08       Impact factor: 5.736

Review 2.  Structure and function of voltage-sensitive ion channels.

Authors:  W A Catterall
Journal:  Science       Date:  1988-10-07       Impact factor: 47.728

3.  Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.

Authors:  M A Frohman; M K Dush; G R Martin
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205

4.  Primary structure of rat brain sodium channel III deduced from the cDNA sequence.

Authors:  T Kayano; M Noda; V Flockerzi; H Takahashi; S Numa
Journal:  FEBS Lett       Date:  1988-02-08       Impact factor: 4.124

5.  Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification.

Authors:  H J Lüdecke; G Senger; U Claussen; B Horsthemke
Journal:  Nature       Date:  1989-03-23       Impact factor: 49.962

6.  Sodium channels from human brain RNA expressed in Xenopus oocytes. Basic electrophysiologic characteristics and their modification by diphenylhydantoin.

Authors:  G F Tomaselli; E Marban; G Yellen
Journal:  J Clin Invest       Date:  1989-05       Impact factor: 14.808

7.  Amplification and analysis of DNA sequences in single human sperm and diploid cells.

Authors:  H H Li; U B Gyllensten; X F Cui; R K Saiki; H A Erlich; N Arnheim
Journal:  Nature       Date:  1988-09-29       Impact factor: 49.962

8.  A comprehensive set of sequence analysis programs for the VAX.

Authors:  J Devereux; P Haeberli; O Smithies
Journal:  Nucleic Acids Res       Date:  1984-01-11       Impact factor: 16.971

9.  Solubilization of sodium channel from human brain.

Authors:  C de Rycker; C Grandfils; L Bettendorff; E Schoffeniels
Journal:  J Neurochem       Date:  1989-02       Impact factor: 5.372

10.  The proximity of DNA sequences in interphase cell nuclei is correlated to genomic distance and permits ordering of cosmids spanning 250 kilobase pairs.

Authors:  B Trask; D Pinkel; G van den Engh
Journal:  Genomics       Date:  1989-11       Impact factor: 5.736
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  8 in total

1.  Primary structure, chromosomal localization, and functional expression of a voltage-gated sodium channel from human brain.

Authors:  C M Ahmed; D H Ware; S C Lee; C D Patten; A V Ferrer-Montiel; A F Schinder; J D McPherson; C B Wagner-McPherson; J J Wasmuth; G A Evans
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-01       Impact factor: 11.205

2.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1991-05-11       Impact factor: 16.971

3.  Band-specific localization of the microsatellite at D13S71 by microdissection and enzymatic amplification.

Authors:  H Spielvogel; H C Hennies; U Claussen; S S Washington; A Chakravarti; A Reis
Journal:  Am J Hum Genet       Date:  1992-05       Impact factor: 11.025

Review 4.  Structure, function and expression of voltage-dependent sodium channels.

Authors:  R G Kallen; S A Cohen; R L Barchi
Journal:  Mol Neurobiol       Date:  1993 Fall-Winter       Impact factor: 5.590

5.  Chromosomal assignment of seven rat homeobox genes to rat chromosomes 3, 4, 7, and 10.

Authors:  S Y Chung; P H Dai; J Lei; M Riviere; G Levan; J Szpirer; C Szpirer
Journal:  Mamm Genome       Date:  1993-09       Impact factor: 2.957

6.  Targeted gene walking by low stringency polymerase chain reaction: assignment of a putative human brain sodium channel gene (SCN3A) to chromosome 2q24-31.

Authors:  M S Malo; K Srivastava; J M Andresen; X N Chen; J R Korenberg; V M Ingram
Journal:  Proc Natl Acad Sci U S A       Date:  1994-04-12       Impact factor: 11.205

7.  A second locus for familial generalized epilepsy with febrile seizures plus maps to chromosome 2q21-q33.

Authors:  S Baulac; I Gourfinkel-An; F Picard; M Rosenberg-Bourgin; J F Prud'homme; M Baulac; A Brice; E LeGuern
Journal:  Am J Hum Genet       Date:  1999-10       Impact factor: 11.025

8.  The alpha-subunit of the skeletal muscle sodium channel is encoded proximal to Tk-1 on mouse chromosome 11.

Authors:  C Ambrose; S Cheng; B Fontaine; J H Nadeau; M MacDonald; J F Gusella
Journal:  Mamm Genome       Date:  1992       Impact factor: 2.957
  8 in total

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