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Literature DB >> 209856

Binding assay for opioid peptides with neuroblastoma x glioma hybrid cells: specificity of the receptor site.

L D Gerber, S Stein, M Rubinstein, J Wideman, S Udenfriend.

Abstract

A sensitive and rapid radioreceptor assay has been developed to monitor opioid peptides in column effluents. It is based on competitive binding to NG 108-15 cells using [3H-Tyr]Leu-enkephalin as the displaced ligand. The specificity of binding to the cells of naturally occurring opioid peptides and synthetic analogs has been shown to be similar to that found with synaptic plasma membranes.

Entities: Disease Gene

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Year: 1978 PMID： 209856 DOI： 10.1016/0006-8993(78)90954-x

Source DB: PubMed Journal: Brain Res ISSN： 0006-8993 Impact factor: 3.252

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Cited

13 in total

1. Purification to homogeneity of camel pituitary pro-opiocortin, the common precursor of opioid peptides and corticotropin.

Authors: S Kimura; R V Lewis; L D Gerber; L Brink; M Rubinstein; S Stein; S Udenfriend
Journal: Proc Natl Acad Sci U S A Date: 1979-04 Impact factor: 11.205

2. Probable precursors of [Leu]enkephalin and [Met]enkephalin in adrenal medulla: peptides of 3-5 kilodaltons.

Authors: S Kimura; R V Lewis; A S Stern; J Rossier; S Stein; S Udenfriend
Journal: Proc Natl Acad Sci U S A Date: 1980-03 Impact factor: 11.205

Review 3. Function of delta opioid receptors in cultured cells.

Authors: J A Gilbert; E Richelson
Journal: Mol Cell Biochem Date: 1983 Impact factor: 3.396

4. Rimorphin, a unique, naturally occurring [Leu]enkephalin-containing peptide found in association with dynorphin and alpha-neo-endorphin.

Authors: D L Kilpatrick; A Wahlstrom; H W Lahm; R Blacher; S Udenfriend
Journal: Proc Natl Acad Sci U S A Date: 1982-11 Impact factor: 11.205

5. Enkephalin biosynthetic pathway: a 5300-dalton adrenal polypeptide that terminates at its COOH end with the sequence [Met]enkephalin-Arg-Gly-Leu-COOH.

Authors: B N Jones; J E Shively; D L Kilpatrick; K Kojima; S Udenfriend
Journal: Proc Natl Acad Sci U S A Date: 1982-02 Impact factor: 11.205

Binding assay for opioid peptides with neuroblastoma x glioma hybrid cells: specificity of the receptor site.

1. Purification to homogeneity of camel pituitary pro-opiocortin, the common precursor of opioid peptides and corticotropin.

2. Probable precursors of [Leu]enkephalin and [Met]enkephalin in adrenal medulla: peptides of 3-5 kilodaltons.

Review 3. Function of delta opioid receptors in cultured cells.

4. Rimorphin, a unique, naturally occurring [Leu]enkephalin-containing peptide found in association with dynorphin and alpha-neo-endorphin.

5. Enkephalin biosynthetic pathway: a 5300-dalton adrenal polypeptide that terminates at its COOH end with the sequence [Met]enkephalin-Arg-Gly-Leu-COOH.

6. An 18,200-dalton adrenal protein that contains four [Met]enkephalin sequences.

7. Adrenal opioid proteins of 8600 and 12,600 daltons: intermediates in proenkephalin processing.

8. Two adrenal opioid polypeptides: proposed intermediates in the processing of proenkephalin.

9. Isolation of the opioid heptapeptide Met-enkephalin [Arg6,Phe7] from bovine adrenal medullary granules and striatum.

10. Evidence for a proenkephalin-like precursor in amphibian brain.