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Literature DB >> 6951177

Enkephalin biosynthetic pathway: a 5300-dalton adrenal polypeptide that terminates at its COOH end with the sequence [Met]enkephalin-Arg-Gly-Leu-COOH.

B N Jones, J E Shively, D L Kilpatrick, K Kojima, S Udenfriend.

Abstract

An enkephalin-containing polypeptide of 5300 daltons has been isolated from extracts of bovine adrenal medulla and purified to homogeneity, and its sequence has been determined by a combination of automated Edman degradation and enzymatic time-course hydrolysis. The polypeptide contains a single [Met]enkephalin sequence followed by Arg-Gly-Leu-COOH, which forms the COOH terminus of the molecule. The enkephalin sequence is preceded by a Lys-Arg linkage, typical of prohormone cleavage sites. Posttranslational processing of the polypeptide at this site would lead to release of the octapeptide [Met]enkephalin-Arg6-Gly7-Leu8.

Entities: Chemical Species

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Year: 1982 PMID： 6951177 PMCID： PMC345953 DOI： 10.1073/pnas.79.4.1313

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

28 in total

1. Automatic Monitoring of primary amines in preparative column effluents with fluorescamine.

Authors: P Böhlen; S Stein; J Stone; S Udenfriend
Journal: Anal Biochem Date: 1975-08 Impact factor: 3.365

2. Characterization of proteins and peptides by high-performance liquid chromatography and fluorescence monitoring of their tryptic digests.

Authors: M Rubinstein; S Chen-Kiang; S Stein; S Udenfriend
Journal: Anal Biochem Date: 1979-05 Impact factor: 3.365

3. Advances in the analysis of amino acid phenylthiohydantoins by high performance liquid chromatography.

Authors: C L Zimmerman; E Appella; J J Pisano
Journal: Anal Biochem Date: 1976-09 Impact factor: 3.365

4. Binding assay for opioid peptides with neuroblastoma x glioma hybrid cells: specificity of the receptor site.

Authors: L D Gerber; S Stein; M Rubinstein; J Wideman; S Udenfriend
Journal: Brain Res Date: 1978-07-28 Impact factor: 3.252

5. Amino acid sequence studies on ten ribosomal proteins of Escherichia coli with an improved sequenator equipped with an automatic conversion device.

Authors: B Wittmann-Liebold
Journal: Hoppe Seylers Z Physiol Chem Date: 1973 Oct-Nov

6. Amino acid analysis with fluorescamine at the picomole level.

Authors: S Stein; P Böhlen; J Stone; W Dairman; S Udenfriend
Journal: Arch Biochem Biophys Date: 1973-03 Impact factor: 4.013

7. Primary structural requirements for the enzymatic formation of the N-glycosidic bond in glycoproteins. Studies with alpha-lactalbumin.

Authors: D K Struck; W J Lennarz; K Brew
Journal: J Biol Chem Date: 1978-08-25 Impact factor: 5.157

3. Distribution and characterization of the opioid octapeptide met5-enkephalin-arg6-gly7-leu8 in the gastrointestinal tract of the rat.

Authors: Y N Wang; I Lindberg
Journal: Cell Tissue Res Date: 1986 Impact factor: 5.249

4. Distribution and functional significance of Met-enkephalin-Arg6-Phe7- and Met-enkephalin-Arg6-Gly7-Leu8-like peptides in the blowfly Calliphora vomitoria. I. Immunocytochemical mapping of neuronal pathways in the brain.

Authors: H Duve; A Thorpe
Journal: Cell Tissue Res Date: 1989-10 Impact factor: 5.249

4 in total

Enkephalin biosynthetic pathway: a 5300-dalton adrenal polypeptide that terminates at its COOH end with the sequence [Met]enkephalin-Arg-Gly-Leu-COOH.

1. Automatic Monitoring of primary amines in preparative column effluents with fluorescamine.

2. Characterization of proteins and peptides by high-performance liquid chromatography and fluorescence monitoring of their tryptic digests.

3. Advances in the analysis of amino acid phenylthiohydantoins by high performance liquid chromatography.

4. Binding assay for opioid peptides with neuroblastoma x glioma hybrid cells: specificity of the receptor site.

5. Amino acid sequence studies on ten ribosomal proteins of Escherichia coli with an improved sequenator equipped with an automatic conversion device.

6. Amino acid analysis with fluorescamine at the picomole level.

7. Primary structural requirements for the enzymatic formation of the N-glycosidic bond in glycoproteins. Studies with alpha-lactalbumin.

8. Detection of two endorphin-like peptides in nucleus caudatus.

9. Putative enkephalin precursors in bovine adrenal medulla.

10. Enzymatic conversion of proteins to glycoproteins.

1. An 18,200-dalton adrenal protein that contains four [Met]enkephalin sequences.

2. Adrenal opioid proteins of 8600 and 12,600 daltons: intermediates in proenkephalin processing.

3. Distribution and characterization of the opioid octapeptide met5-enkephalin-arg6-gly7-leu8 in the gastrointestinal tract of the rat.

4. Distribution and functional significance of Met-enkephalin-Arg6-Phe7- and Met-enkephalin-Arg6-Gly7-Leu8-like peptides in the blowfly Calliphora vomitoria. I. Immunocytochemical mapping of neuronal pathways in the brain.