BACKGROUND: Sickle cell disease or drepanocytosis is caused by the polymerisation of abnormal haemoglobin S when oxygen tension decreases. This lead to the changes in the shape of red blood cells and anaemia. It has also been postulated that the red cells of patients with sickle cell disease contain a higher than normal concentration of calcium ions. These ions are bound to membrane proteins resulting in dehydration and loss of red blood cell deformability and cell-to-cell adherence. Anthocyanins extracted from some Congolese plants used in traditional medicine against sickle cell disease have recently been shown to have anti-sickling activity in vitro. Justicia secunda is a plant used in Congo by Jehovah's Witnesses, well known for their refusal of blood transfusions, against anaemia. MATERIALS AND METHODS: Emmel, Itano and osmotic fragility tests were used to test the effect of anthocyanin extracts from Justicia secunda leaves on haemoglobin S solubility and sickle cell membrane stability. RESULTS: Anthocyanins from Justicia secunda were found to possess anti-sickling activity. Treated SS red blood cells recovered a normal, classical biconcave form with a radius of 3.3±0.3 μm, similar to that of normal erythrocytes. The solubility of deoxyhaemoglobin S increased and the osmotic fragility of drepanocytes decreased upon treatment with anthocyanin extracts. CONCLUSION: These findings suggest that anthocyanin extracts play a role in both stabilising the red blood cell membrane and inhibiting polymerisation of haemoglobin S. This provides a possible molecular basis for earlier reports on the anti-sickling properties of anthocyanins from some Congolese plants and their use in the management of sickle cell disease by Congolese traditional healers.
BACKGROUND:Sickle cell disease or drepanocytosis is caused by the polymerisation of abnormal haemoglobin S when oxygen tension decreases. This lead to the changes in the shape of red blood cells and anaemia. It has also been postulated that the red cells of patients with sickle cell disease contain a higher than normal concentration of calcium ions. These ions are bound to membrane proteins resulting in dehydration and loss of red blood cell deformability and cell-to-cell adherence. Anthocyanins extracted from some Congolese plants used in traditional medicine against sickle cell disease have recently been shown to have anti-sickling activity in vitro. Justicia secunda is a plant used in Congo by Jehovah's Witnesses, well known for their refusal of blood transfusions, against anaemia. MATERIALS AND METHODS: Emmel, Itano and osmotic fragility tests were used to test the effect of anthocyanin extracts from Justicia secunda leaves on haemoglobin S solubility and sickle cell membrane stability. RESULTS:Anthocyanins from Justicia secunda were found to possess anti-sickling activity. Treated SS red blood cells recovered a normal, classical biconcave form with a radius of 3.3±0.3 μm, similar to that of normal erythrocytes. The solubility of deoxyhaemoglobin S increased and the osmotic fragility of drepanocytes decreased upon treatment with anthocyanin extracts. CONCLUSION: These findings suggest that anthocyanin extracts play a role in both stabilising the red blood cell membrane and inhibiting polymerisation of haemoglobin S. This provides a possible molecular basis for earlier reports on the anti-sickling properties of anthocyanins from some Congolese plants and their use in the management of sickle cell disease by Congolese traditional healers.
Authors: P T Mpiana; V Mudogo; D S T Tshibangu; E K Kitwa; A B Kanangila; J B S Lumbu; K N Ngbolua; E K Atibu; M K Kakule Journal: J Ethnopharmacol Date: 2008-09-23 Impact factor: 4.360
Authors: Luiz Marivando Barros; Antonia Eliene Duarte; Emily Pansera Waczuk; Katiane Roversi; Francisco Assis Bezerra da Cunha; Mirian Rolon; Cathia Coronel; Maria Celeste Vega Gomez; Irwin Rose Alencar de Menezes; José Galberto Martins da Costa; Aline Augusti Boligon; Waseem Hassan; Diogo Onofre Souza; João Batista Teixeira da Rocha; Jean Paul Kamdem Journal: EXCLI J Date: 2017-04-21 Impact factor: 4.068
Authors: Shahida A Khan; Ghazi Damanhouri; Ashraf Ali; Sarah A Khan; Aziz Khan; Ahmed Bakillah; Samy Marouf; Ghazi Al Harbi; Saeed H Halawani; Ahmad Makki Journal: Nutr Metab (Lond) Date: 2016-08-08 Impact factor: 4.169