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Literature DB >> 20953177

Dual RMCE for efficient re-engineering of mouse mutant alleles.

Marco Osterwalder¹, Antonella Galli, Barry Rosen, William C Skarnes, Rolf Zeller, Javier Lopez-Rios.

Abstract

We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.

Entities: Chemical Disease Gene Mutation Species

Mesh：

Substances：

Year: 2010 PMID： 20953177 PMCID： PMC3576631 DOI： 10.1038/nmeth.1521

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

14 in total

1. Stable and efficient cassette exchange under non-selectable conditions by combined use of two site-specific recombinases.

Authors: Matthias Lauth; Fabio Spreafico; Kathrin Dethleffsen; Michael Meyer
Journal: Nucleic Acids Res Date: 2002-11-01 Impact factor: 16.971

Review 2. Talking about a revolution: The impact of site-specific recombinases on genetic analyses in mice.

Authors: Catherine S Branda; Susan M Dymecki
Journal: Dev Cell Date: 2004-01 Impact factor: 12.270

3. Differential requirements for Smad4 in TGFbeta-dependent patterning of the early mouse embryo.

Authors: Gerald C Chu; N Ray Dunn; Dorian C Anderson; Leif Oxburgh; Elizabeth J Robertson
Journal: Development Date: 2004-06-23 Impact factor: 6.868

4. A mouse for all reasons.

Authors: Francis S Collins; Janet Rossant; Wolfgang Wurst
Journal: Cell Date: 2007-01-12 Impact factor: 41.582

Review 5. Current issues in mouse genome engineering.

Authors: Stefan Glaser; Konstantinos Anastassiadis; A Francis Stewart
Journal: Nat Genet Date: 2005-11 Impact factor: 38.330

6. Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells.

Authors: T Kanda; K F Sullivan; G M Wahl
Journal: Curr Biol Date: 1998-03-26 Impact factor: 10.834

7. Codon-improved Cre recombinase (iCre) expression in the mouse.

Authors: D R Shimshek; J Kim; M R Hübner; D J Spergel; F Buchholz; E Casanova; A F Stewart; P H Seeburg; R Sprengel
Journal: Genesis Date: 2002-01 Impact factor: 2.487

8. Distinct roles of Hand2 in initiating polarity and posterior Shh expression during the onset of mouse limb bud development.

Authors: Antonella Galli; Dimitri Robay; Marco Osterwalder; Xiaozhong Bao; Jean-Denis Bénazet; Muhammad Tariq; Renato Paro; Susan Mackem; Rolf Zeller
Journal: PLoS Genet Date: 2010-04-08 Impact factor: 5.917

9. Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector.

Authors: Andrea L Szymczak; Creg J Workman; Yao Wang; Kate M Vignali; Smaroula Dilioglou; Elio F Vanin; Dario A A Vignali
Journal: Nat Biotechnol Date: 2004-04-04 Impact factor: 54.908

10. A public gene trap resource for mouse functional genomics.

Authors: William C Skarnes; Harald von Melchner; Wolfgang Wurst; Geoff Hicks; Alex S Nord; Tony Cox; Stephen G Young; Patricia Ruiz; Phil Soriano; Marc Tessier-Lavigne; Bruce R Conklin; William L Stanford; Janet Rossant
Journal: Nat Genet Date: 2004-06 Impact factor: 38.330

38 in total

1. The author file: Rolf Zeller and Javier Lopez-Rios.

Authors: Monya Baker
Journal: Nat Methods Date: 2010-11 Impact factor: 28.547

2. The transience of transient overexpression.

Authors: Toby J Gibson; Markus Seiler; Reiner A Veitia
Journal: Nat Methods Date: 2013-08 Impact factor: 28.547

3. Resources for proteomics in mouse embryonic stem cells.

Authors: Frank Schnütgen; Franziska Ehrmann; Ina Poser; Nina C Hubner; Jens Hansen; Thomas Floss; Ingrid deVries; Wolfgang Wurst; Anthony Hyman; Matthias Mann; Harald von Melchner
Journal: Nat Methods Date: 2011-02 Impact factor: 28.547

Review 4. Mouse genetic and phenotypic resources for human genetics.

Authors: Paul N Schofield; Robert Hoehndorf; Georgios V Gkoutos
Journal: Hum Mutat Date: 2012-05 Impact factor: 4.878

5. Functions of the COPII gene paralogs SEC23A and SEC23B are interchangeable in vivo.

Authors: Rami Khoriaty; Geoffrey G Hesketh; Amélie Bernard; Angela C Weyand; Dattatreya Mellacheruvu; Guojing Zhu; Mark J Hoenerhoff; Beth McGee; Lesley Everett; Elizabeth J Adams; Bin Zhang; Thomas L Saunders; Alexey I Nesvizhskii; Daniel J Klionsky; Jordan A Shavit; Anne-Claude Gingras; David Ginsburg
Journal: Proc Natl Acad Sci U S A Date: 2018-07-31 Impact factor: 11.205