Literature DB >> 23900254

The transience of transient overexpression.

Toby J Gibson1, Markus Seiler, Reiner A Veitia.   

Abstract

Much of what is known about mammalian cell regulation has been achieved with the aid of transiently transfected cells. However, overexpression can violate balanced gene dosage, affecting protein folding, complex assembly and downstream regulation. To avoid these problems, genome engineering technologies now enable the generation of stable cell lines expressing modified proteins at (almost) native levels.

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Mesh：

Year:  2013        PMID： 23900254     DOI： 10.1038/nmeth.2534

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  69 in total

Review 1.  Protein folding and misfolding.

Authors:  Christopher M Dobson
Journal:  Nature       Date:  2003-12-18       Impact factor: 49.962

2.  Structural characterisation and functional significance of transient protein-protein interactions.

Authors:  Irene M A Nooren; Janet M Thornton
Journal:  J Mol Biol       Date:  2003-01-31       Impact factor: 5.469

3.  Generalized lacZ expression with the ROSA26 Cre reporter strain.

Authors:  P Soriano
Journal:  Nat Genet       Date:  1999-01       Impact factor: 38.330

4.  The switches.ELM resource: a compendium of conditional regulatory interaction interfaces.

Authors:  Kim Van Roey; Holger Dinkel; Robert J Weatheritt; Toby J Gibson; Norman E Davey
Journal:  Sci Signal       Date:  2013-04-02       Impact factor: 8.192

5.  Structural basis for the cytoskeletal association of Bcr-Abl/c-Abl.

Authors:  Oliver Hantschel; Silke Wiesner; Thomas Güttler; Cameron D Mackereth; Lily L Remsing Rix; Zsuzsanna Mikes; Jana Dehne; Dirk Görlich; Michael Sattler; Giulio Superti-Furga
Journal:  Mol Cell       Date:  2005-08-19       Impact factor: 17.970

6.  Jalview Version 2--a multiple sequence alignment editor and analysis workbench.

Authors:  Andrew M Waterhouse; James B Procter; David M A Martin; Michèle Clamp; Geoffrey J Barton
Journal:  Bioinformatics       Date:  2009-01-16       Impact factor: 6.937

7.  Identification of constitutive and ras-inducible phosphorylation sites of KSR: implications for 14-3-3 binding, mitogen-activated protein kinase binding, and KSR overexpression.

Authors:  A M Cacace; N R Michaud; M Therrien; K Mathes; T Copeland; G M Rubin; D K Morrison
Journal:  Mol Cell Biol       Date:  1999-01       Impact factor: 4.272

8.  The quantitative proteome of a human cell line.

Authors:  Martin Beck; Alexander Schmidt; Johan Malmstroem; Manfred Claassen; Alessandro Ori; Anna Szymborska; Franz Herzog; Oliver Rinner; Jan Ellenberg; Ruedi Aebersold
Journal:  Mol Syst Biol       Date:  2011-11-08       Impact factor: 11.429

Review 9.  From RNAi screens to molecular function in embryonic stem cells.

Authors:  Li Ding; Ina Poser; Maciej Paszkowski-Rogacz; Frank Buchholz
Journal:  Stem Cell Rev Rep       Date:  2012-03       Impact factor: 5.739

10.  The International Gene Trap Consortium Website: a portal to all publicly available gene trap cell lines in mouse.

Authors:  Alex S Nord; Patricia J Chang; Bruce R Conklin; Antony V Cox; Courtney A Harper; Geoffrey G Hicks; Conrad C Huang; Susan J Johns; Michiko Kawamoto; Songyan Liu; Elaine C Meng; John H Morris; Janet Rossant; Patricia Ruiz; William C Skarnes; Philippe Soriano; William L Stanford; Doug Stryke; Harald von Melchner; Wolfgang Wurst; Ken-ichi Yamamura; Stephen G Young; Patricia C Babbitt; Thomas E Ferrin
Journal:  Nucleic Acids Res       Date:  2006-01-01       Impact factor: 16.971
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  85 in total

1.  Dual-tagged amyloid-β precursor protein reveals distinct transport pathways of its N- and C-terminal fragments.

Authors:  Christine Villegas; Virgil Muresan; Zoia Ladescu Muresan
Journal:  Hum Mol Genet       Date:  2013-11-07       Impact factor: 6.150

2.  An improved toolbox to unravel the plant cellular machinery by tandem affinity purification of Arabidopsis protein complexes.

Authors:  Jelle Van Leene; Dominique Eeckhout; Bernard Cannoot; Nancy De Winne; Geert Persiau; Eveline Van De Slijke; Leen Vercruysse; Maarten Dedecker; Aurine Verkest; Klaas Vandepoele; Lennart Martens; Erwin Witters; Kris Gevaert; Geert De Jaeger
Journal:  Nat Protoc       Date:  2014-12-18       Impact factor: 13.491

3.  Co-Immunoprecipitation of Membrane-Bound Receptors.

Authors:  Julian R Avila; Jin Suk Lee; Keiko U Torii
Journal:  Arabidopsis Book       Date:  2015-06-03

Review 4.  On the cutting edge: protease-based methods for sensing and controlling cell biology.

Authors:  H Kay Chung; Michael Z Lin
Journal:  Nat Methods       Date:  2020-07-13       Impact factor: 28.547

Review 5.  To be disordered or not to be disordered: is that still a question for proteins in the cell?

Authors:  Kris Pauwels; Pierre Lebrun; Peter Tompa
Journal:  Cell Mol Life Sci       Date:  2017-06-13       Impact factor: 9.261

6.  Elution profile analysis of SDS-induced subcomplexes by quantitative mass spectrometry.

Authors:  Yves Texier; Grischa Toedt; Matteo Gorza; Dorus A Mans; Jeroen van Reeuwijk; Nicola Horn; Jason Willer; Nicholas Katsanis; Ronald Roepman; Toby J Gibson; Marius Ueffing; Karsten Boldt
Journal:  Mol Cell Proteomics       Date:  2014-02-21       Impact factor: 5.911

7.  Development of a stable cell line with an intact PGC-1α/ERRα axis for screening environmental chemicals.

Authors:  Christina T Teng; Burton Beames; B Alex Merrick; Negin Martin; Charles Romeo; Anton M Jetten
Journal:  Biochem Biophys Res Commun       Date:  2014-01-20       Impact factor: 3.575

8.  Molecular Mechanisms of Class B GPCR Activation: Insights from Adrenomedullin Receptors.

Authors:  Michael L Garelja; Maggie Au; Margaret A Brimble; Joseph J Gingell; Erica R Hendrikse; Annie Lovell; Nicole Prodan; Patrick M Sexton; Andrew Siow; Christopher S Walker; Harriet A Watkins; Geoffrey M Williams; Denise Wootten; Sung H Yang; Paul W R Harris; Debbie L Hay
Journal:  ACS Pharmacol Transl Sci       Date:  2020-02-26

Review 9.  Emerging potential of gene silencing approaches targeting anti-chondrogenic factors for cell-based cartilage repair.

Authors:  Andrea Lolli; Letizia Penolazzi; Roberto Narcisi; Gerjo J V M van Osch; Roberta Piva
Journal:  Cell Mol Life Sci       Date:  2017-04-22       Impact factor: 9.261

Review 10.  Light Microscopy of Mitochondria at the Nanoscale.

Authors:  Stefan Jakobs; Till Stephan; Peter Ilgen; Christian Brüser
Journal:  Annu Rev Biophys       Date:  2020-02-24       Impact factor: 12.981
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