Literature DB >> 20729841

An antibiotic selection marker for nematode transgenesis.

Rosina Giordano-Santini1, Stuart Milstein, Nenad Svrzikapa, Domena Tu, Robert Johnsen, David Baillie, Marc Vidal, Denis Dupuy.   

Abstract

We have developed a nematode transformation vector carrying the bacterial neomycin resistance gene (NeoR) and shown that it could confer resistance to G-418 on both wild-type Caenorhabditis elegans and C. briggsae. This selection system allows hands-off maintenance and enrichment of transgenic worms carrying non-integrated transgenes on selective plates. We also show that this marker can be used for Mos1-mediated single-copy insertion in wild-type genetic backgrounds (MosSCI-biotic).

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Year:  2010        PMID： 20729841     DOI： 10.1038/nmeth.1494

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  14 in total

1.  Creation of low-copy integrated transgenic lines in Caenorhabditis elegans.

Authors:  V Praitis; E Casey; D Collar; J Austin
Journal:  Genetics       Date:  2001-03       Impact factor: 4.562

2.  Caenorhabditis elegans dpy-5 is a cuticle procollagen processed by a proprotein convertase.

Authors:  C Thacker; J A Sheps; A M Rose
Journal:  Cell Mol Life Sci       Date:  2006-05       Impact factor: 9.261

3.  pha-1, a selectable marker for gene transfer in C. elegans.

Authors:  M Granato; H Schnabel; R Schnabel
Journal:  Nucleic Acids Res       Date:  1994-05-11       Impact factor: 16.971

4.  A new dominant hybrid selective marker for higher eukaryotic cells.

Authors:  F Colbère-Garapin; F Horodniceanu; P Kourilsky; A C Garapin
Journal:  J Mol Biol       Date:  1981-07-25       Impact factor: 5.469

5.  Transgene-mediated cosuppression in the C. elegans germ line.

Authors:  A F Dernburg; J Zalevsky; M P Colaiácovo; A M Villeneuve
Journal:  Genes Dev       Date:  2000-07-01       Impact factor: 11.361

6.  A first version of the Caenorhabditis elegans Promoterome.

Authors:  Denis Dupuy; Qian-Ru Li; Bart Deplancke; Mike Boxem; Tong Hao; Philippe Lamesch; Reynaldo Sequerra; Stephanie Bosak; Lynn Doucette-Stamm; Ian A Hope; David E Hill; Albertha J M Walhout; Marc Vidal
Journal:  Genome Res       Date:  2004-10       Impact factor: 9.043

7.  Molecular cloning of a dominant roller mutant and establishment of DNA-mediated transformation in the nematode Pristionchus pacificus.

Authors:  Benjamin Schlager; Xiaoyue Wang; Georg Braach; Ralf J Sommer
Journal:  Genesis       Date:  2009-05       Impact factor: 2.487

8.  Mos1 transposition as a tool to engineer the Caenorhabditis elegans genome by homologous recombination.

Authors:  Valérie J P Robert; Iskra Katic; Jean-Louis Bessereau
Journal:  Methods       Date:  2009-02-27       Impact factor: 3.608

9.  Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences.

Authors:  C C Mello; J M Kramer; D Stinchcomb; V Ambros
Journal:  EMBO J       Date:  1991-12       Impact factor: 11.598

10.  Single-copy insertion of transgenes in Caenorhabditis elegans.

Authors:  Christian Frøkjaer-Jensen; M Wayne Davis; Christopher E Hopkins; Blake J Newman; Jason M Thummel; Søren-Peter Olesen; Morten Grunnet; Erik M Jorgensen
Journal:  Nat Genet       Date:  2008-10-26       Impact factor: 38.330
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  38 in total

1.  Generating transgenic nematodes by bombardment and antibiotic selection.

Authors:  Jennifer I Semple; Laura Biondini; Ben Lehner
Journal:  Nat Methods       Date:  2012-01-30       Impact factor: 28.547

Review 2.  The early bird catches the worm: new technologies for the Caenorhabditis elegans toolkit.

Authors:  Xiao Xu; Stuart K Kim
Journal:  Nat Rev Genet       Date:  2011-10-04       Impact factor: 53.242

Review 3.  From genes to function: the C. elegans genetic toolbox.

Authors:  Thomas Boulin; Oliver Hobert
Journal:  Wiley Interdiscip Rev Dev Biol       Date:  2011-11-28       Impact factor: 5.814

4.  Prototypic chromatin insulator cHS4 protects retroviral transgene from silencing in Schistosoma mansoni.

Authors:  Sutas Suttiprapa; Gabriel Rinaldi; Paul J Brindley
Journal:  Transgenic Res       Date:  2011-09-15       Impact factor: 2.788

Review 5.  Forward and reverse mutagenesis in C. elegans.

Authors:  Lena M Kutscher; Shai Shaham
Journal:  WormBook       Date:  2014-01-17

6.  High-throughput optical quantification of mechanosensory habituation reveals neurons encoding memory in Caenorhabditis elegans.

Authors:  Takuma Sugi; Yasuko Ohtani; Yuta Kumiya; Ryuji Igarashi; Masahiro Shirakawa
Journal:  Proc Natl Acad Sci U S A       Date:  2014-11-17       Impact factor: 11.205

Review 7.  Genetic and epigenetic effects on centromere establishment.

Authors:  Yick Hin Ling; Zhongyang Lin; Karen Wing Yee Yuen
Journal:  Chromosoma       Date:  2019-11-28       Impact factor: 4.316

Review 8.  The Caenorhabditis elegans Transgenic Toolbox.

Authors:  Jeremy Nance; Christian Frøkjær-Jensen
Journal:  Genetics       Date:  2019-08       Impact factor: 4.562

9.  Purine Homeostasis Is Necessary for Developmental Timing, Germline Maintenance and Muscle Integrity in Caenorhabditis elegans.

Authors:  Roxane Marsac; Benoît Pinson; Christelle Saint-Marc; María Olmedo; Marta Artal-Sanz; Bertrand Daignan-Fornier; José-Eduardo Gomes
Journal:  Genetics       Date:  2019-01-30       Impact factor: 4.562

10.  Developmental Sensitivity in Schistosoma mansoni to Puromycin To Establish Drug Selection of Transgenic Schistosomes.

Authors:  Hong-Bin Yan; Michael J Smout; Chuan Ju; Anne E Folley; Danielle E Skinner; Victoria H Mann; Alex Loukas; Wei Hu; Paul J Brindley; Gabriel Rinaldi
Journal:  Antimicrob Agents Chemother       Date:  2018-07-27       Impact factor: 5.191
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