Literature DB >> 20697597

Dielectrophoresis of DNA: Quantification by impedance measurements.

Anja Henning1, Frank F Bier, Ralph Hölzel.   

Abstract

Dielectrophoretic properties of DNA have been determined by measuring capacitance changes between planar microelectrodes. DNA sizes ranged from 100 bp to 48 kbp, DNA concentrations from below 0.1 to 70 mugml. Dielectrophoretic spectra exhibited maximum response around 3 kHz and 3 MHz. The strongest response was found for very long DNA (above 10 kbp) and for short 100 bp fragments, which corresponds to the persistence length of DNA. The method allows for an uncomplicated, automatic acquisition of the dielectrophoretic properties of submicroscopical objects without the need for labeling protocols or optical accessibility.

Entities:  

Year:  2010        PMID： 20697597      PMCID： PMC2917884          DOI： 10.1063/1.3430550

Source DB:  PubMed          Journal:  Biomicrofluidics        ISSN： 1932-1058            Impact factor:   2.800


  20 in total

1.  Fluid flow induced by nonuniform ac electric fields in electrolytes on microelectrodes. I. Experimental measurements

Authors: 
Journal:  Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics       Date:  2000-04

2.  EFFECTS OF HIGH-FREQUENCY ELECTRIC FIELDS ON THE LIVING CELL. I. BEHAVIOUR OF HUMAN ERYTHROCYTES IN HIGH-FREQUENCY ELECTRIC FIELDS AND ITS RELATION TO THEIR AGE.

Authors:  A A FUEREDI; I OHAD
Journal:  Biochim Biophys Acta       Date:  1964-01-27

3.  Fluid flow induced by nonuniform ac electric fields in electrolytes on microelectrodes. III. Observation of streamlines and numerical simulation.

Authors:  N G Green; A Ramos; A González; H Morgan; A Castellanos
Journal:  Phys Rev E Stat Nonlin Soft Matter Phys       Date:  2002-08-19

4.  Dielectric characterization of bacterial cells using dielectrophoresis.

Authors:  A Sanchis; A P Brown; M Sancho; G Martínez; J L Sebastián; S Muñoz; J M Miranda
Journal:  Bioelectromagnetics       Date:  2007-07       Impact factor: 2.010

5.  Synthesis and stretching of rolling circle amplification products in a flow-through system.

Authors:  Edda Reiss; Ralph Hölzel; Frank F Bier
Journal:  Small       Date:  2009-10       Impact factor: 13.281

6.  Traveling-wave electrokinetic micropumps: velocity, electrical current, and impedance measurements.

Authors:  P García-Sánchez; A Ramos; N G Green; H Morgan
Journal:  Langmuir       Date:  2008-08-02       Impact factor: 3.882

7.  Enhancing DNA hybridization kinetics through constriction-based dielectrophoresis.

Authors:  Nathan Swami; Chia-Fu Chou; Venkatraman Ramamurthy; Vasudha Chaurey
Journal:  Lab Chip       Date:  2009-09-08       Impact factor: 6.799

8.  Visualization of single molecules of RNA polymerase sliding along DNA.

Authors:  H Kabata; O Kurosawa; I Arai; M Washizu; S A Margarson; R E Glass; N Shimamoto
Journal:  Science       Date:  1993-12-03       Impact factor: 47.728

9.  Applications of a new optical technique for measuring the dielectrophoretic behaviour of micro-organisms.

Authors:  J A Price; J P Burt; R Pethig
Journal:  Biochim Biophys Acta       Date:  1988-02-17

10.  Label-free electrical quantification of the dielectrophoretic response of DNA.

Authors:  Anja Henning; Jörg Henkel; Frank F Bier; Ralph Hölzel
Journal:  PMC Biophys       Date:  2008-11-05
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  17 in total

1.  Floating-electrode enhanced constriction dielectrophoresis for biomolecular trapping in physiological media of high conductivity.

Authors:  Vasudha Chaurey; Carlos Polanco; Chia-Fu Chou; Nathan S Swami
Journal:  Biomicrofluidics       Date:  2012-03-15       Impact factor: 2.800

2.  Preface to special topic: dielectrophoresis.

Authors:  Ronald Pethig
Journal:  Biomicrofluidics       Date:  2010-06-29       Impact factor: 2.800

3.  Manipulating single annealed polyelectrolyte under alternating current electric fields: Collapse versus accumulation.

Authors:  Shengqin Wang; Yingxi Zhu
Journal:  Biomicrofluidics       Date:  2012-05-01       Impact factor: 2.800

4.  Enhancement of continuous-flow separation of viable/nonviable yeast cells using a nonuniform alternating current electric field with complex spatial distribution.

Authors:  Shigeru Tada; Arisa Nakanishi; Masanori Eguchi; Kengo Ochi; Megumi Baba; Akira Tsukamoto
Journal:  Biomicrofluidics       Date:  2016-05-20       Impact factor: 2.800

5.  Dielectrophoretic manipulation of ribosomal RNA.

Authors:  Gerard Giraud; Ronald Pethig; Holger Schulze; Grace Henihan; Jonathan G Terry; Anoop Menachery; Ilenia Ciani; Damion Corrigan; Colin J Campbell; Andrew R Mount; Peter Ghazal; Anthony J Walton; Jason Crain; Till T Bachmann
Journal:  Biomicrofluidics       Date:  2011-06-28       Impact factor: 2.800

6.  High-throughput separation of cells by dielectrophoresis enhanced with 3D gradient AC electric field.

Authors:  Shigeru Tada; Masako Hayashi; Masanori Eguchi; Akira Tsukamoto
Journal:  Biomicrofluidics       Date:  2017-12-13       Impact factor: 2.800

Review 7.  Protein dielectrophoresis and the link to dielectric properties.

Authors:  Fernanda Camacho-Alanis; Alexandra Ros
Journal:  Bioanalysis       Date:  2015       Impact factor: 2.681

8.  Measuring Nanoparticle Polarizability Using Fluorescence Microscopy.

Authors:  Wenhan Cao; Margaret Chern; Allison M Dennis; Keith A Brown
Journal:  Nano Lett       Date:  2019-07-22       Impact factor: 11.189

9.  Transitioning Streaming to Trapping in DC Insulator-based Dielectrophoresis for Biomolecules.

Authors:  Fernanda Camacho-Alanis; Lin Gan; Alexandra Ros
Journal:  Sens Actuators B Chem       Date:  2012-10       Impact factor: 7.460

Review 10.  Protein dielectrophoresis: advances, challenges, and applications.

Authors:  Asuka Nakano; Alexandra Ros
Journal:  Electrophoresis       Date:  2013-04       Impact factor: 3.535
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