PURPOSE: a laser is commonly used to remove a blastomere from an embryo for genetic testing. The laser uses intense heat which could possibly disrupt embryo development. It is the goal of this study to test the effects of different laser pulse lengths (and consequently heat) on the embryo biopsy procedure and embryo development. METHODS: each embryo biopsy was performed randomly utilizing laser pulse lengths of 0.604mS (group I), 0.708mS (group II), and 1.010mS (group III). RESULTS: for groups I, II, and III, 83, 86, and 71 embryos were biopsied, respectively. There was no difference in day 5 embryo quality or lysed blastomeres between groups. Average number of blastomeres biopsied between group I (1.0 ± 0.0), II (1.0 ± 0.2), and III (1.1 ± 0.2) was significant (0.0001). CONCLUSION: our data demonstrates that laser pulse length does not influence the embryo biopsy procedure or embryo development.
RCT Entities:
PURPOSE: a laser is commonly used to remove a blastomere from an embryo for genetic testing. The laser uses intense heat which could possibly disrupt embryo development. It is the goal of this study to test the effects of different laser pulse lengths (and consequently heat) on the embryo biopsy procedure and embryo development. METHODS: each embryo biopsy was performed randomly utilizing laser pulse lengths of 0.604mS (group I), 0.708mS (group II), and 1.010mS (group III). RESULTS: for groups I, II, and III, 83, 86, and 71 embryos were biopsied, respectively. There was no difference in day 5 embryo quality or lysed blastomeres between groups. Average number of blastomeres biopsied between group I (1.0 ± 0.0), II (1.0 ± 0.2), and III (1.1 ± 0.2) was significant (0.0001). CONCLUSION: our data demonstrates that laser pulse length does not influence the embryo biopsy procedure or embryo development.
Authors: Antonio Capalbo; Valeria Romanelli; Danilo Cimadomo; Laura Girardi; Marta Stoppa; Lisa Dovere; Domenico Dell'Edera; Filippo Maria Ubaldi; Laura Rienzi Journal: J Assist Reprod Genet Date: 2016-07-16 Impact factor: 3.412
Authors: Maria Cristina Santoro Biazotti; Walter Pinto Junior; Maria Cecília Romano Maciel de Albuquerque; Litsuko Shimabukuro Fujihara; Cláudia Haru Suganuma; Renata Bednar Reigota; Carmen Sílvia Bertuzzo Journal: Einstein (Sao Paulo) Date: 2015 Jan-Mar
Authors: Inna V Ilina; Yulia V Khramova; Anna D Ivanova; Maxim A Filatov; Yuliya Yu Silaeva; Alexey V Deykin; Dmitry S Sitnikov Journal: J Assist Reprod Genet Date: 2020-11-17 Impact factor: 3.412
Authors: Danilo Cimadomo; Antonio Capalbo; Filippo Maria Ubaldi; Catello Scarica; Antonio Palagiano; Rita Canipari; Laura Rienzi Journal: Biomed Res Int Date: 2016-01-28 Impact factor: 3.411