Ehab Darwish1, Yasmin Magdi2. 1. TopLab Company for ART Laboratories Consultations and Training, Ground floor, 27 Zohair Sabry st., 1st district, Nasr City, Cairo, Egypt. 2. TopLab Company for ART Laboratories Consultations and Training, Ground floor, 27 Zohair Sabry st., 1st district, Nasr City, Cairo, Egypt. Yas.magdi@hotmail.com.
Abstract
PURPOSE: Blastocysts contain a large amount of fluid in the blastocoel, which may pose a risk for ice crystal formation during vitrification. This study aimed to evaluate the effectiveness of laser-induced artificial shrinkage of blastocoel before vitrification on clinical outcome. METHODS: Patients were divided into two groups: a control group with untreated, expanded blastocysts (n = 115) and a study group with blastocoel artificially eliminated by a laser pulse prior to vitrification (n = 309). Blastocyst survival, clinical pregnancy, and implantation rates were compared. RESULT(S): The survival rate was significantly higher in the study group compared with the control group (97.3 and 74.9 %, respectively; p > 0.01). The clinical pregnancy and implantation rates of the study group were significantly higher (p < 0.01) than that of the control group (clinical pregnancy, 67.2 vs. 41.1 %; implantation, 39.1 vs. 24.5 %. CONCLUSION(S): This study demonstrated that the removal of blastocoel fluid before vitrification by laser pulse of in vitro-produced human blastocysts significantly improves blastocyst survival, clinical pregnancy, and implantation rates.
PURPOSE:Blastocysts contain a large amount of fluid in the blastocoel, which may pose a risk for ice crystal formation during vitrification. This study aimed to evaluate the effectiveness of laser-induced artificial shrinkage of blastocoel before vitrification on clinical outcome. METHODS:Patients were divided into two groups: a control group with untreated, expanded blastocysts (n = 115) and a study group with blastocoel artificially eliminated by a laser pulse prior to vitrification (n = 309). Blastocyst survival, clinical pregnancy, and implantation rates were compared. RESULT(S): The survival rate was significantly higher in the study group compared with the control group (97.3 and 74.9 %, respectively; p > 0.01). The clinical pregnancy and implantation rates of the study group were significantly higher (p < 0.01) than that of the control group (clinical pregnancy, 67.2 vs. 41.1 %; implantation, 39.1 vs. 24.5 %. CONCLUSION(S): This study demonstrated that the removal of blastocoel fluid before vitrification by laser pulse of in vitro-produced humanblastocysts significantly improves blastocyst survival, clinical pregnancy, and implantation rates.
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