Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 A photoactivatable marker protein for pulse-chase imaging with superresolution.

Literature DB >> 20601949

A photoactivatable marker protein for pulse-chase imaging with superresolution.

Jochen Fuchs¹, Susan Böhme, Franz Oswald, Per Niklas Hedde, Maike Krause, Jörg Wiedenmann, G Ulrich Nienhaus.

Abstract

IrisFP is a photoactivatable fluorescent protein that combines irreversible photoconversion from a green- to a red-emitting form with reversible photoswitching between a fluorescent and a nonfluorescent state in both forms. Here we introduce a monomeric variant, mIrisFP, and demonstrate how its multiple photoactivation modes can be used for pulse-chase experiments combined with subdiffraction-resolution imaging in living cells by using dual-color photoactivation localization microscopy (PALM).

Mesh：

Substances：

Year: 2010 PMID： 20601949 DOI： 10.1038/nmeth.1477

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

24 in total

Review 1. Adhesion assembly, disassembly and turnover in migrating cells -- over and over and over again.

Authors: Donna J Webb; J Thomas Parsons; Alan F Horwitz
Journal: Nat Cell Biol Date: 2002-04 Impact factor: 28.824

2. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.

Authors: Nathan C Shaner; Robert E Campbell; Paul A Steinbach; Ben N G Giepmans; Amy E Palmer; Roger Y Tsien
Journal: Nat Biotechnol Date: 2004-11-21 Impact factor: 54.908

Review 3. Live-cell imaging with EosFP and other photoactivatable marker proteins of the GFP family.

Authors: Jörg Wiedenmann; G Ulrich Nienhaus
Journal: Expert Rev Proteomics Date: 2006-06 Impact factor: 3.940

Review 4. Advances in fluorescent protein technology.

Authors: Nathan C Shaner; George H Patterson; Michael W Davidson
Journal: J Cell Sci Date: 2007-12-15 Impact factor: 5.285

5. Optimized and far-red-emitting variants of fluorescent protein eqFP611.

Authors: Simone Kredel; Karin Nienhaus; Franz Oswald; Michael Wolff; Sergey Ivanchenko; Florian Cymer; Andreas Jeromin; Francois J Michel; Klaus-Dieter Spindler; Ralf Heilker; G Ulrich Nienhaus; Jörg Wiedenmann
Journal: Chem Biol Date: 2008-03

6. Microscopy and its focal switch.

Authors: Stefan W Hell
Journal: Nat Methods Date: 2009-01 Impact factor: 28.547

7. Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics.

Authors: Hari Shroff; Catherine G Galbraith; James A Galbraith; Eric Betzig
Journal: Nat Methods Date: 2008-04-13 Impact factor: 28.547

8. Structural characterization of IrisFP, an optical highlighter undergoing multiple photo-induced transformations.

Authors: Virgile Adam; Mickaël Lelimousin; Susan Boehme; Guillaume Desfonds; Karin Nienhaus; Martin J Field; Joerg Wiedenmann; Sean McSweeney; G Ulrich Nienhaus; Dominique Bourgeois
Journal: Proc Natl Acad Sci U S A Date: 2008-11-18 Impact factor: 11.205

9. Sulfasalazine: a potent and specific inhibitor of nuclear factor kappa B.

Authors: C Wahl; S Liptay; G Adler; R M Schmid
Journal: J Clin Invest Date: 1998-03-01 Impact factor: 14.808

10. NF-kappaB2 is a putative target gene of activated Notch-1 via RBP-Jkappa.

Authors: F Oswald; S Liptay; G Adler; R M Schmid
Journal: Mol Cell Biol Date: 1998-04 Impact factor: 4.272

37 in total

1. Dual color photoactivation localization microscopy of cardiomyopathy-associated desmin mutants.

Authors: Andreas Brodehl; Per Niklas Hedde; Mareike Dieding; Azra Fatima; Volker Walhorn; Susan Gayda; Tomo Šarić; Bärbel Klauke; Jan Gummert; Dario Anselmetti; Mike Heilemann; Gerd Ulrich Nienhaus; Hendrik Milting
Journal: J Biol Chem Date: 2012-03-08 Impact factor: 5.157

A photoactivatable marker protein for pulse-chase imaging with superresolution.

Review 1. Adhesion assembly, disassembly and turnover in migrating cells -- over and over and over again.

2. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.

Review 3. Live-cell imaging with EosFP and other photoactivatable marker proteins of the GFP family.

Review 4. Advances in fluorescent protein technology.

5. Optimized and far-red-emitting variants of fluorescent protein eqFP611.

6. Microscopy and its focal switch.

7. Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics.

8. Structural characterization of IrisFP, an optical highlighter undergoing multiple photo-induced transformations.

9. Sulfasalazine: a potent and specific inhibitor of nuclear factor kappa B.

10. NF-kappaB2 is a putative target gene of activated Notch-1 via RBP-Jkappa.

1. Dual color photoactivation localization microscopy of cardiomyopathy-associated desmin mutants.

Review 2. Photophysics of fluorescent probes for single-molecule biophysics and super-resolution imaging.

Review 3. Proteins on the move: insights gained from fluorescent protein technologies.

Review 4. Systems biology in immunology: a computational modeling perspective.

5. Rational design of true monomeric and bright photoactivatable fluorescent proteins.

Review 6. Super-resolution localization microscopy with photoactivatable fluorescent marker proteins.

Review 7. High-throughput single-molecule studies of protein-DNA interactions.

Review 8. Genetically encodable fluorescent biosensors for tracking signaling dynamics in living cells.

Review 9. Chromophore chemistry of fluorescent proteins controlled by light.

Review 10. Chemical Biology Framework to Illuminate Proteostasis.