| Literature DB >> 20583594 |
B Ostash1, I Ostash, L Zhu, M K Kharel, A Luzhetskiĭ, A Bechthold, S Walker, J Rohr, V Fedorenko.
Abstract
LanK is TetR-like regulatory protein recently shown to regulate the export and glycosylation of landomycins in Streptomyces cyanogenus S136. Here, several properties of the lanK-mediated regulation were deciphered. LanK seems to function as oligomer as evident from experiments in vitro. In vivo, it is able to recognize various landomycins with altered aglycon structure and the minimal concentration of landomycin A sensed by LanK lies in low nanomolar range. Coexpression studies showed that the positive regulatory gene lanI upregulates lanK-dependent lan genes once the negative LanK-regulation is cancelled. Gene lanK can be useful for the construction of biosensor strains for sensitive and specific identification of producers of landomycin-like molecules with long glycosidic chains.Entities:
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Year: 2010 PMID： 20583594 PMCID： PMC2905788
Source DB: PubMed Journal: Genetika ISSN： 0016-6758