Literature DB >> 2057350

High level heterologous expression in E. coli using the anaerobically-activated nirB promoter.

M D Oxer1, C M Bentley, J G Doyle, T C Peakman, I G Charles, A J Makoff.   

Abstract

The anaerobically-regulated nirB promoter was used to express heterologous genes in Escherichia coli. Under anaerobic conditions the promoter was able to express tetanus toxin fragment C at approximately 20% total cell protein (tcp) and the Bordetella pertussis antigen pertactin at greater than 30% tcp. These levels are comparable to those obtained for the same products using the tac promoter. The nirB promoter is very well regulated, giving almost two orders of magnitude increase in fragment C on complete removal of oxygen. The use of this anaerobically-induced promoter in the production of recombinant proteins in E. coli is discussed.

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Year:  1991        PMID： 2057350      PMCID： PMC328247          DOI： 10.1093/nar/19.11.2889

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  22 in total

1.  Molecular genetic analysis of an FNR-dependent anaerobically inducible Escherichia coli promoter.

Authors:  A I Bell; J A Cole; S J Busby
Journal:  Mol Microbiol       Date:  1990-10       Impact factor: 3.501

2.  Cytochrome c552 and nitrite reduction in Escherichia coli.

Authors:  J A Cole
Journal:  Biochim Biophys Acta       Date:  1968-10-01

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Authors:  M Meselson; R Yuan
Journal:  Nature       Date:  1968-03-23       Impact factor: 49.962

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda.

Authors:  E Remaut; P Stanssens; W Fiers
Journal:  Gene       Date:  1981-10       Impact factor: 3.688

6.  A family of cloning vectors containing the lacUV5 promoter.

Authors:  F Fuller
Journal:  Gene       Date:  1982 Jul-Aug       Impact factor: 3.688

7.  Plasmid vectors containing the tryptophan operon promoter suitable for efficient regulated expression of foreign genes.

Authors:  R A Hallewell; S Emtage
Journal:  Gene       Date:  1980-04       Impact factor: 3.688

8.  The construction and characterisation of plasmid vectors suitable for the expression of all DNA phases under the control of the E. coli tryptophan promoter.

Authors:  W Tacon; N Carey; S Emtage
Journal:  Mol Gen Genet       Date:  1980-02

9.  The tac promoter: a functional hybrid derived from the trp and lac promoters.

Authors:  H A de Boer; L J Comstock; M Vasser
Journal:  Proc Natl Acad Sci U S A       Date:  1983-01       Impact factor: 11.205

10.  Expression of recombinant proteins in Escherichia coli using an oxygen-responsive promoter.

Authors:  C Khosla; J E Curtis; P Bydalek; J R Swartz; J E Bailey
Journal:  Biotechnology (N Y)       Date:  1990-06
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  22 in total

1.  Optimization of plasmid maintenance in the attenuated live vector vaccine strain Salmonella typhi CVD 908-htrA.

Authors:  J E Galen; J Nair; J Y Wang; S S Wasserman; M K Tanner; M B Sztein; M M Levine
Journal:  Infect Immun       Date:  1999-12       Impact factor: 3.441

2.  Expression and immunogenicity of a mutant diphtheria toxin molecule, CRM(197), and its fragments in Salmonella typhi vaccine strain CVD 908-htrA.

Authors:  N Orr; J E Galen; M M Levine
Journal:  Infect Immun       Date:  1999-08       Impact factor: 3.441

Review 3.  Review: optimizing inducer and culture conditions for expression of foreign proteins under the control of the lac promoter.

Authors:  R S Donovan; C W Robinson; B R Glick
Journal:  J Ind Microbiol       Date:  1996-03

4.  Expression and immunogenicity of pertussis toxin S1 subunit-tetanus toxin fragment C fusions in Salmonella typhi vaccine strain CVD 908.

Authors:  E M Barry; O Gomez-Duarte; S Chatfield; R Rappuoli; M Pizza; G Losonsky; J Galen; M M Levine
Journal:  Infect Immun       Date:  1996-10       Impact factor: 3.441

Review 5.  Understanding the art of producing protein and nonprotein molecules in Escherichia coli.

Authors:  P Balbás
Journal:  Mol Biotechnol       Date:  2001-11       Impact factor: 2.695

6.  Salmonella enterica serovar Typhimurium vaccine strains expressing a nontoxic Shiga-like toxin 2 derivative induce partial protective immunity to the toxin expressed by enterohemorrhagic Escherichia coli.

Authors:  Robert L G Rojas; Priscila A D P Gomes; Leticia V Bentancor; Maria E Sbrogio-Almeida; Sérgio O P Costa; Liliana M Massis; Rita C C Ferreira; Marina S Palermo; Luís C S Ferreira
Journal:  Clin Vaccine Immunol       Date:  2010-02-10

7.  Construction and characterization of a Salmonella enterica serovar typhimurium clone expressing a salivary adhesin of Streptococcus mutans under control of the anaerobically inducible nirB promoter.

Authors:  Y Huang; G Hajishengallis; S M Michalek
Journal:  Infect Immun       Date:  2000-03       Impact factor: 3.441

8.  Vaccine efficacy of Salmonella strains expressing glycoprotein 63 with different promoters.

Authors:  S J McSorley; D Xu; F Y Liew
Journal:  Infect Immun       Date:  1997-01       Impact factor: 3.441

9.  Heterologous expression and maturation of an NADP-dependent [NiFe]-hydrogenase: a key enzyme in biofuel production.

Authors:  Junsong Sun; Robert C Hopkins; Francis E Jenney; Patrick M McTernan; Michael W W Adams
Journal:  PLoS One       Date:  2010-05-06       Impact factor: 3.240

10.  Anaerobically controlled expression system derived from the arcDABC operon of Pseudomonas aeruginosa: application to lipase production.

Authors:  H V Winteler; B Schneidinger; K E Jaeger; D Haas
Journal:  Appl Environ Microbiol       Date:  1996-09       Impact factor: 4.792
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