Literature DB >> 8652113

Review: optimizing inducer and culture conditions for expression of foreign proteins under the control of the lac promoter.

R S Donovan1, C W Robinson, B R Glick.   

Abstract

This review examines factors which influence the expression of foreign proteins in Escherichia coli under the transcriptional control of the lac and tac promoters, and discusses conditions for maximizing the production of a foreign protein using this system. Specifically, the influence of IPTG (isopropyl-beta-D-thiogalactoside) concentration, temperature, composition of the growth medium, the point in the growth curve at which cells are induced with either IPTG or lactose, and the duration of the induction phase are discussed.

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Year:  1996        PMID： 8652113     DOI： 10.1007/bf01569997

Source DB:  PubMed          Journal:  J Ind Microbiol        ISSN： 0169-4146


  75 in total

1.  Ribosomal protein limitations in Escherichia coli under conditions of high translational activity.

Authors:  L Laffend; M L Shuler
Journal:  Biotechnol Bioeng       Date:  1994-03-05       Impact factor: 4.530

2.  Expression of recombinant protein A from the lac promoter in Escherichia coli JM83 is not subject to catabolite repression when grown under specific conditions of continuous culture.

Authors:  A Warnes; J R Stephenson; A R Fooks; J Melling; M R Brown
Journal:  Biotechnol Bioeng       Date:  1991-11       Impact factor: 4.530

Review 3.  The role of the PEP: carbohydrate phosphotransferase system in the regulation of bacterial metabolism.

Authors:  P W Postma; C P Broekhuizen; R H Geerse
Journal:  FEMS Microbiol Rev       Date:  1989-06       Impact factor: 16.408

4.  Continuous production of cell-free recombinant proteins using Escherichia coli.

Authors:  P Yu; K Y San
Journal:  Biotechnol Prog       Date:  1993 Nov-Dec

5.  The effect of beta-galactosides on the protonmotive force and growth of Escherichia coli.

Authors:  S Ahmed; I R Booth
Journal:  J Gen Microbiol       Date:  1983-08

6.  Production of L-dihydroxyphenylalanine in Escherichia coli with the tyrosine phenol-lyase gene cloned from Erwinia herbicola.

Authors:  F Foor; N Morin; K A Bostian
Journal:  Appl Environ Microbiol       Date:  1993-09       Impact factor: 4.792

7.  Induction of T4 DNA ligase in a recombinant strain of Escherichia coli.

Authors:  G K Whitney; B R Glick; C W Robinson
Journal:  Biotechnol Bioeng       Date:  1989-03       Impact factor: 4.530

8.  The effect of folding catalysts on the in vivo folding process of different antibody fragments expressed in Escherichia coli.

Authors:  A Knappik; C Krebber; A Plückthun
Journal:  Biotechnology (N Y)       Date:  1993-01

9.  Genetically structured models forlac promoter-operator function in the Escherichia coli chromosome and in multicopy plasmids: Lac operator function.

Authors:  S B Lee; J E Bailey
Journal:  Biotechnol Bioeng       Date:  1984-11       Impact factor: 4.530

10.  Promoters of Escherichia coli: a hierarchy of in vivo strength indicates alternate structures.

Authors:  U Deuschle; W Kammerer; R Gentz; H Bujard
Journal:  EMBO J       Date:  1986-11       Impact factor: 11.598
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  75 in total

1.  Computational design of a Zn2+ receptor that controls bacterial gene expression.

Authors:  M A Dwyer; L L Looger; H W Hellinga
Journal:  Proc Natl Acad Sci U S A       Date:  2003-09-19       Impact factor: 11.205

2.  Combining the hok/sok, parDE, and pnd postsegregational killer loci to enhance plasmid stability.

Authors:  D C Pecota; C S Kim; K Wu; K Gerdes; T K Wood
Journal:  Appl Environ Microbiol       Date:  1997-05       Impact factor: 4.792

3.  High-performance recombinant protein production with Escherichia coli in continuously operated cascades of stirred-tank reactors.

Authors:  Andreas Schmideder; Dirk Weuster-Botz
Journal:  J Ind Microbiol Biotechnol       Date:  2017-03-01       Impact factor: 3.346

4.  Characterizing Protein Kinase Substrate Specificity Using the Proteomic Peptide Library (ProPeL) Approach.

Authors:  Joshua M Lubner; Jeremy L Balsbaugh; George M Church; Michael F Chou; Daniel Schwartz
Journal:  Curr Protoc Chem Biol       Date:  2018-06

5.  Production of hepatopoietin Cn in high-cell-density cultures of recombinant Escherichia coli and detection of its antioxygen activity.

Authors:  Yang Liu; Xiao-Li Li; Dong-Dong Zhang; Qiang Wang; Yong Liu; Dong-Mei Liu; Chu-Tse Wu; Chun-Ping Cui
Journal:  Mol Biotechnol       Date:  2011-02       Impact factor: 2.695

6.  New constitutive vectors: useful genetic engineering tools for biocatalysis.

Authors:  Youqiang Xu; Fei Tao; Cuiqing Ma; Ping Xu
Journal:  Appl Environ Microbiol       Date:  2013-02-15       Impact factor: 4.792

7.  Replication methods and tools in high-throughput cultivation processes - recognizing potential variations of growth and product formation by on-line monitoring.

Authors:  Robert Huber; Thomas G Palmen; Nadine Ryk; Anne-Kathrin Hillmer; Karina Luft; Frank Kensy; Jochen Büchs
Journal:  BMC Biotechnol       Date:  2010-03-16       Impact factor: 2.563

8.  Production of glycoprotein vaccines in Escherichia coli.

Authors:  Julian Ihssen; Michael Kowarik; Sandro Dilettoso; Cyril Tanner; Michael Wacker; Linda Thöny-Meyer
Journal:  Microb Cell Fact       Date:  2010-08-11       Impact factor: 5.328

9.  High cell density cultivation of a recombinant E. coli strain expressing a key enzyme in bioengineered heparin production.

Authors:  Odile Francesca Restaino; Ujjwal Bhaskar; Priscilla Paul; Lingyun Li; Mario De Rosa; Jonathan S Dordick; Robert J Linhardt
Journal:  Appl Microbiol Biotechnol       Date:  2013-01-15       Impact factor: 4.813

10.  Robo-Lector - a novel platform for automated high-throughput cultivations in microtiter plates with high information content.

Authors:  Robert Huber; Daniel Ritter; Till Hering; Anne-Kathrin Hillmer; Frank Kensy; Carsten Müller; Le Wang; Jochen Büchs
Journal:  Microb Cell Fact       Date:  2009-08-01       Impact factor: 5.328
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