Literature DB >> 20557039

Precise mapping of RNA tertiary structure via nanometer distance measurements with double electron-electron resonance spectroscopy.

Nak-Kyoon Kim1, Michael K Bowman, Victoria J DeRose.   

Abstract

Divalent metal (Mg(2+)) ion-dependent folding of the hammerhead ribozyme from Schistosoma mansoni was monitored with double electron-electron resonance (DEER) pulsed electron paramagnetic resonance spectroscopy by measuring nanometer-scale distances between paramagnetic spin-labels attached to the RNA. DEER measurements detect global folding of the ribozyme with excellent agreement between predictions from experimental, modeled, and crystallographic measurements. These measurements demonstrate the use of DEER spectroscopy as a tool for structural analysis of complex RNAs.

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Year:  2010        PMID: 20557039      PMCID: PMC2908488          DOI: 10.1021/ja101317g

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN: 0002-7863            Impact factor:   15.419


  31 in total

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5.  Motions of the substrate recognition duplex in a group I intron assessed by site-directed spin labeling.

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6.  High-resolution distance mapping in rhodopsin reveals the pattern of helix movement due to activation.

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7.  Relative orientation of rigid nitroxides by PELDOR: beyond distance measurements in nucleic acids.

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8.  Metal ion specificities for folding and cleavage activity in the Schistosoma hammerhead ribozyme.

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  14 in total

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2.  Doubly Spin-Labeled RNA as an EPR Reporter for Studying Multicomponent Supramolecular Assemblies.

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Journal:  Biophys J       Date:  2015-12-15       Impact factor: 4.033

Review 3.  RNA dynamics: perspectives from spin labels.

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Journal:  Wiley Interdiscip Rev RNA       Date:  2011-08-22       Impact factor: 9.957

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5.  Site-directed spin-labeling of nucleotides and the use of in-cell EPR to determine long-range distances in a biologically relevant environment.

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7.  Investigating the position of the hairpin loop in New Delhi metallo-β-lactamase, NDM-1, during catalysis and inhibitor binding.

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8.  Global structure of a three-way junction in a phi29 packaging RNA dimer determined using site-directed spin labeling.

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9.  Pulsed EPR distance measurements in soluble proteins by site-directed spin labeling (SDSL).

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10.  Structural rearrangements in mRNA upon its binding to human 80S ribosomes revealed by EPR spectroscopy.

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Journal:  Nucleic Acids Res       Date:  2018-01-25       Impact factor: 16.971

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