Literature DB >> 19322852

Relative orientation of rigid nitroxides by PELDOR: beyond distance measurements in nucleic acids.

Olav Schiemann1, Pavol Cekan, Dominik Margraf, Thomas F Prisner, Snorri Th Sigurdsson.   

Abstract

Show me your angle: Incorporation of the rigid spin label C allows determination of both distance and orientation of two nitroxide spin labels in DNA by PELDOR experiments at common X-band frequencies. The orientational information is obtained by varying the position of the detection pulses over the nitroxide spectrum. Simulation of the set of time traces yields very precise distances and angles.

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Year:  2009        PMID: 19322852     DOI: 10.1002/anie.200805152

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN: 1433-7851            Impact factor:   15.336


  33 in total

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5.  Site-directed spin-labeling of nucleotides and the use of in-cell EPR to determine long-range distances in a biologically relevant environment.

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8.  Precise mapping of RNA tertiary structure via nanometer distance measurements with double electron-electron resonance spectroscopy.

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9.  Pulsed EPR distance measurements in soluble proteins by site-directed spin labeling (SDSL).

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Journal:  Curr Protoc Protein Sci       Date:  2013-11-05

10.  Probing the (H3-H4)2 histone tetramer structure using pulsed EPR spectroscopy combined with site-directed spin labelling.

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Journal:  Nucleic Acids Res       Date:  2009-11-13       Impact factor: 16.971

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