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Literature DB >> 2053284

cis Acting RNA sequences control the gag-pol translation readthrough in murine leukemia virus.

A Honigman¹, D Wolf, S Yaish, H Falk, A Panet.

Abstract

The pol gene of the Moloney murine leukemia virus (M-MuLV) is expressed as a Gag-Pol fusion protein through an in-frame suppression of the UAG termination codon located between the two genes. The role of nucleotide context in suppression was investigated, in a rabbit reticulocyte lysate translation system, using site-directed mutagenesis. The results indicate that the translational readthrough is mediated by at least 50 bases long RNA sequence located 3' to the gag UAG termination codon. Within this sequence a short purine-rich sequence adjacent to the amber codon, highly conserved among different retroviruses, appears essential for M-MuLV suppression. Two alternative putative stem and loop like RNA structures can be drawn at the gag-pol junction, one abutting the gag UAG codon, and the second downstream to it. None of these structures appears to be important to the suppression process.

Entities: Chemical Disease Gene Species

Mesh：

Substances：

Year: 1991 PMID： 2053284 PMCID： PMC7131665 DOI： 10.1016/0042-6822(91)90144-z

Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616

31 in total

1. Translation of MuLV and MSV RNAs in nuclease-treated reticulocyte extracts: enhancement of the gag-pol polypeptide with yeast suppressor tRNA.

Authors: L Philipson; P Andersson; U Olshevsky; R Weinberg; D Baltimore; R Gesteland
Journal: Cell Date: 1978-01 Impact factor: 41.582

2. Expression of the gag-pol fusion protein of Moloney murine leukemia virus without gag protein does not induce virion formation or proteolytic processing.

Authors: K M Felsenstein; S P Goff
Journal: J Virol Date: 1988-06 Impact factor: 5.103

3. Characterization of ribosomal frameshifting in HIV-1 gag-pol expression.

Authors: T Jacks; M D Power; F R Masiarz; P A Luciw; P J Barr; H E Varmus
Journal: Nature Date: 1988-01-21 Impact factor: 49.962

4. Natural UAG suppressor glutamine tRNA is elevated in mouse cells infected with Moloney murine leukemia virus.

Authors: Y Kuchino; H Beier; N Akita; S Nishimura
Journal: Proc Natl Acad Sci U S A Date: 1987-05 Impact factor: 11.205

5. Nucleotide sequence of Moloney murine leukaemia virus.

Authors: T M Shinnick; R A Lerner; J G Sutcliffe
Journal: Nature Date: 1981 Oct 15-21 Impact factor: 49.962

6. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.

Authors: D A Melton; P A Krieg; M R Rebagliati; T Maniatis; K Zinn; M R Green
Journal: Nucleic Acids Res Date: 1984-09-25 Impact factor: 16.971

7. Context effects: translation of UAG codon by suppressor tRNA is affected by the sequence following UAG in the message.

Authors: L Bossi
Journal: J Mol Biol Date: 1983-02-15 Impact factor: 5.469

8. Deletion mutants of Moloney murine leukemia virus which lack glycosylated gag protein are replication competent.

Authors: P Schwartzberg; J Colicelli; S P Goff
Journal: J Virol Date: 1983-05 Impact factor: 5.103

9. RNA pseudoknots: translational frameshifting and readthrough on viral RNAs.

Authors: E B ten Dam; C W Pleij; L Bosch
Journal: Virus Genes Date: 1990-07 Impact factor: 2.332

10. Characterization of an efficient coronavirus ribosomal frameshifting signal: requirement for an RNA pseudoknot.

Authors: I Brierley; P Digard; S C Inglis
Journal: Cell Date: 1989-05-19 Impact factor: 41.582

25 in total

Review 1. Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs.

Authors: H Beier; M Grimm
Journal: Nucleic Acids Res Date: 2001-12-01 Impact factor: 16.971

2. Expression and processing of proteins encoded by the Saccharomyces retrotransposon Ty5.

Authors: P A Irwin; D F Voytas
Journal: J Virol Date: 2001-02 Impact factor: 5.103

3. The soybean retroelement SIRE1 uses stop codon suppression to express its envelope-like protein.

Authors: Ericka R Havecker; Daniel F Voytas
Journal: EMBO Rep Date: 2003-03 Impact factor: 8.807

4. Mutational analysis of the gag-pol junction of Moloney murine leukemia virus: requirements for expression of the gag-pol fusion protein.

Authors: K M Felsenstein; S P Goff
Journal: J Virol Date: 1992-11 Impact factor: 5.103

5. Bipartite signal for read-through suppression in murine leukemia virus mRNA: an eight-nucleotide purine-rich sequence immediately downstream of the gag termination codon followed by an RNA pseudoknot.

Authors: Y X Feng; H Yuan; A Rein; J G Levin
Journal: J Virol Date: 1992-08 Impact factor: 5.103

10. An RNA Element That Facilitates Programmed Ribosomal Readthrough in Turnip Crinkle Virus Adopts Multiple Conformations.

Authors: Micki M Kuhlmann; Maitreyi Chattopadhyay; Vera A Stupina; Feng Gao; Anne E Simon
Journal: J Virol Date: 2016-09-12 Impact factor: 5.103