Anna Yudina1, Matthieu Lepetit-Coiffé, Chrit T W Moonen. 1. Laboratory for Molecular and Functional Imaging: from Physiology to Therapy, UMR5231 CNRS/University Victor Segalen Bordeaux, 146 rue Leo Saignat, Case 117, 33076, Bordeaux, France. anna@imf.u-bordeaux2.fr
Abstract
PURPOSE: Ultrasound-induced cavitation facilitates cellular uptake of drugs via increased membrane permeability. Here, the purpose was to evaluate the duration of enhanced membrane permeability following ultrasound treatment in cell culture. PROCEDURES: Optical chromophores with fluorescence intensity increasing 100-1,000-fold upon intercalation with nucleic acids served as smart agents for reporting cellular uptake. Opticell chambers with a monolayer of C6 cells were subjected to ultrasound in the presence of microbubbles followed by varying delays between 0 and 24 h before addition of Sytox Green optical contrast agent. Micro- and macroscopic fluorescence were used for qualitative and quantitative analysis. RESULTS: Up to 25% of viable cells showed uptake of contrast agent with a half time of 8 h, with cellular uptake persisting even at 24 h. Only cells exposed to ultrasound showed the effect. CONCLUSION: The temporal window of increased membrane permeability is much longer in these studies than previously suggested. This may have important repercussions for in vivo studies in which membrane permeability may be temporally separated from drug delivery.
PURPOSE: Ultrasound-induced cavitation facilitates cellular uptake of drugs via increased membrane permeability. Here, the purpose was to evaluate the duration of enhanced membrane permeability following ultrasound treatment in cell culture. PROCEDURES: Optical chromophores with fluorescence intensity increasing 100-1,000-fold upon intercalation with nucleic acids served as smart agents for reporting cellular uptake. Opticell chambers with a monolayer of C6 cells were subjected to ultrasound in the presence of microbubbles followed by varying delays between 0 and 24 h before addition of Sytox Green optical contrast agent. Micro- and macroscopic fluorescence were used for qualitative and quantitative analysis. RESULTS: Up to 25% of viable cells showed uptake of contrast agent with a half time of 8 h, with cellular uptake persisting even at 24 h. Only cells exposed to ultrasound showed the effect. CONCLUSION: The temporal window of increased membrane permeability is much longer in these studies than previously suggested. This may have important repercussions for in vivo studies in which membrane permeability may be temporally separated from drug delivery.
Authors: Robyn K Schlicher; Harish Radhakrishna; Timothy P Tolentino; Robert P Apkarian; Vladimir Zarnitsyn; Mark R Prausnitz Journal: Ultrasound Med Biol Date: 2006-06 Impact factor: 2.998
Authors: Michaelann Shortencarier Tartis; Jennifer McCallan; Aaron F H Lum; Rachel LaBell; Susanne M Stieger; Terry O Matsunaga; Katherine W Ferrara Journal: Ultrasound Med Biol Date: 2006-11 Impact factor: 2.998
Authors: Kristin M Dittmar; Jianwu Xie; Finie Hunter; Cameron Trimble; Monica Bur; Victor Frenkel; King C P Li Journal: Radiology Date: 2005-03-29 Impact factor: 11.105
Authors: Y Taniyama; K Tachibana; K Hiraoka; M Aoki; S Yamamoto; K Matsumoto; T Nakamura; T Ogihara; Y Kaneda; R Morishita Journal: Gene Ther Date: 2002-03 Impact factor: 5.250
Authors: John O Larkin; Garrett D Casey; Mark Tangney; James Cashman; Chris G Collins; Declan M Soden; Gerald C O'Sullivan Journal: Ultrasound Med Biol Date: 2007-11-07 Impact factor: 2.998
Authors: Bernadet D M Meijering; Lynda J M Juffermans; Annemieke van Wamel; Rob H Henning; Inge S Zuhorn; Marcia Emmer; Amanda M G Versteilen; Walter J Paulus; Wiek H van Gilst; Klazina Kooiman; Nico de Jong; René J P Musters; Leo E Deelman; Otto Kamp Journal: Circ Res Date: 2009-01-22 Impact factor: 17.367
Authors: Guillaume Lajoinie; Ine De Cock; Constantin C Coussios; Ine Lentacker; Séverine Le Gac; Eleanor Stride; Michel Versluis Journal: Biomicrofluidics Date: 2016-01-28 Impact factor: 2.800