PURPOSE: Transport across the plasma membrane is a critical step of drug delivery for weakly permeable compounds with intracellular mode of action. The purpose of this study is to demonstrate real-time monitoring of ultrasound (US)-mediated cell-impermeable model drug uptake with fibered confocal fluorescence microscopy (FCFM). PROCEDURES: An in vitro setup was designed to combine a mono-element US transducer, a cell chamber with a monolayer of tumor cells together with SonoVue microbubbles, and a FCFM system. The cell-impermeable intercalating dye, SYTOX Green, was used to monitor US-mediated uptake. RESULTS: The majority of the cell population showed fluorescence signal enhancement 10 s after US onset. The mean rate constant k of signal enhancement was calculated to be 0.23 ± 0.04 min(-1). CONCLUSIONS: Feasibility of real-time monitoring of US-mediated intracellular delivery by FCFM has been demonstrated. The method allowed quantitative assessment of model drug uptake, holding great promise for further local drug delivery studies.
PURPOSE: Transport across the plasma membrane is a critical step of drug delivery for weakly permeable compounds with intracellular mode of action. The purpose of this study is to demonstrate real-time monitoring of ultrasound (US)-mediated cell-impermeable model drug uptake with fibered confocal fluorescence microscopy (FCFM). PROCEDURES: An in vitro setup was designed to combine a mono-element US transducer, a cell chamber with a monolayer of tumor cells together with SonoVue microbubbles, and a FCFM system. The cell-impermeable intercalating dye, SYTOX Green, was used to monitor US-mediated uptake. RESULTS: The majority of the cell population showed fluorescence signal enhancement 10 s after US onset. The mean rate constant k of signal enhancement was calculated to be 0.23 ± 0.04 min(-1). CONCLUSIONS: Feasibility of real-time monitoring of US-mediated intracellular delivery by FCFM has been demonstrated. The method allowed quantitative assessment of model drug uptake, holding great promise for further local drug delivery studies.
Authors: Klazina Kooiman; Miranda Foppen-Harteveld; Antonius F W van der Steen; Nico de Jong Journal: J Control Release Date: 2011-04-14 Impact factor: 9.776
Authors: Guillaume Lajoinie; Ine De Cock; Constantin C Coussios; Ine Lentacker; Séverine Le Gac; Eleanor Stride; Michel Versluis Journal: Biomicrofluidics Date: 2016-01-28 Impact factor: 2.800
Authors: B H A Lammertink; R Deckers; M Derieppe; I De Cock; I Lentacker; G Storm; C T W Moonen; C Bos Journal: Mol Imaging Biol Date: 2017-10 Impact factor: 3.488
Authors: Marc Derieppe; Jean-Michel Escoffre; Baudouin Denis de Senneville; Quincy van Houtum; Angelique Barten-van Rijbroek; Kim van der Wurff-Jacobs; Ludwig Dubois; Clemens Bos; Chrit Moonen Journal: Contrast Media Mol Imaging Date: 2019-03-07 Impact factor: 3.161